BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbpv0138 (756 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 58 2e-09 SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-... 55 1e-08 SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizos... 48 2e-06 SPAC19G12.15c |tpp1||trehalose-6-phosphate phosphatase Tpp1|Schi... 36 0.005 SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 33 0.058 SPAP27G11.05c |vps41||vacuolar protein sorting-associated protei... 32 0.077 SPAC1952.10c |||conserved fungal protein |Schizosaccharomyces po... 26 6.7 SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|S... 26 6.7 SPBC839.02 |||arrestin Aly1 related|Schizosaccharomyces pombe|ch... 25 8.8 SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1... 25 8.8 SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 8.8 >SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 944 Score = 57.6 bits (133), Expect = 2e-09 Identities = 30/81 (37%), Positives = 47/81 (58%), Gaps = 1/81 (1%) Frame = +2 Query: 260 DH-WKAYIKINEEFAEKTVHALHLLNKQKKDKNGSPPIVWVHDYHLMLAANWIRQRAEED 436 DH W Y+K+N+ FA+ V N ++ D ++W++DYHL+L +R+R Sbjct: 300 DHSWANYVKVNKAFADTIVD-----NYEQDD------MIWINDYHLLLVPEMVRERLP-- 346 Query: 437 EIKCKLAFFLHIPFPPWDIFR 499 + K+ FFLHIPFP ++FR Sbjct: 347 --RAKIGFFLHIPFPSSEVFR 365 Score = 34.3 bits (75), Expect = 0.019 Identities = 12/36 (33%), Positives = 23/36 (63%) Frame = +1 Query: 517 EVLQGILGCDMVGFHIEDYCLNFIDCCQRNFGLPCR 624 E+L+G+LG +++GF I ++ +F+ C R + R Sbjct: 372 EILKGMLGANILGFQIPEFAYHFLQTCSRLVNIDIR 407 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/23 (43%), Positives = 12/23 (52%), Gaps = 1/23 (4%) Frame = +3 Query: 177 FDSYYNGCCNGTFWPLFH-SMPD 242 F +YN C WP FH +PD Sbjct: 269 FVGHYNHYCKNILWPTFHYQIPD 291 >SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 55.2 bits (127), Expect = 1e-08 Identities = 31/87 (35%), Positives = 45/87 (51%) Frame = +2 Query: 248 HFLADHWKAYIKINEEFAEKTVHALHLLNKQKKDKNGSPPIVWVHDYHLMLAANWIRQRA 427 +F ++W+AY N FAE V N Q D ++WV DYHLM+ +R+ Sbjct: 124 NFDEENWEAYRAANYAFAEAIVK-----NLQDGD------LIWVQDYHLMVLPQMLRELI 172 Query: 428 EEDEIKCKLAFFLHIPFPPWDIFRLFP 508 + K+ FFLH PFP +I+R+ P Sbjct: 173 GDKFKDIKIGFFLHTPFPSSEIYRVLP 199 Score = 46.4 bits (105), Expect = 4e-06 Identities = 17/38 (44%), Positives = 25/38 (65%) Frame = +1 Query: 502 VPGSDEVLQGILGCDMVGFHIEDYCLNFIDCCQRNFGL 615 +P +E+L+G+L CD+VGFH DY +F+ C R L Sbjct: 198 LPVRNEILEGVLNCDLVGFHTYDYARHFLSACSRILNL 235 Score = 37.9 bits (84), Expect = 0.002 Identities = 22/71 (30%), Positives = 32/71 (45%), Gaps = 1/71 (1%) Frame = +3 Query: 45 WVGWPGIHLEDPNEKIPESDPNDKTPTAGLRSE-KIVPIRAEPKLFDSYYNGCCNGTFWP 221 W+GW G ++IPE + L+ E +P+ + + D +YNG N WP Sbjct: 64 WLGWCG-------QEIPEDEK--PMIIQRLQDECSAIPVFLDDETADRHYNGFSNSILWP 114 Query: 222 LFHSMPDRATF 254 LFH P F Sbjct: 115 LFHYHPGEINF 125 >SPAC22F8.05 |||alpha,alpha-trehalose-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 891 Score = 47.6 bits (108), Expect = 2e-06 Identities = 25/81 (30%), Positives = 40/81 (49%) Frame = +2 Query: 263 HWKAYIKINEEFAEKTVHALHLLNKQKKDKNGSPPIVWVHDYHLMLAANWIRQRAEEDEI 442 +W Y+ +N FA+ + K K G +WV+DYHL+L N +R+R Sbjct: 264 NWDDYVAVNRAFADALI---------KNYKTGDT--IWVNDYHLLLVPNMVRERIP---- 308 Query: 443 KCKLAFFLHIPFPPWDIFRLF 505 + F+H+ FP ++FR F Sbjct: 309 SAIIGLFIHVSFPSSEVFRCF 329 Score = 35.9 bits (79), Expect = 0.006 Identities = 13/29 (44%), Positives = 21/29 (72%) Frame = +1 Query: 517 EVLQGILGCDMVGFHIEDYCLNFIDCCQR 603 E+LQG+LG +++GF E+Y +F+ C R Sbjct: 334 ELLQGMLGSNLIGFQTEEYKRHFLQSCSR 362 Score = 29.5 bits (63), Expect = 0.54 Identities = 16/62 (25%), Positives = 27/62 (43%) Frame = +3 Query: 45 WVGWPGIHLEDPNEKIPESDPNDKTPTAGLRSEKIVPIRAEPKLFDSYYNGCCNGTFWPL 224 WVG G+ P E + E N + ++ + +V ++ F+ +YN C WP Sbjct: 193 WVGLLGM----PTESLSEKTKNAISGALLVKHQSLVVYTSDSN-FEGHYNHYCRKILWPS 247 Query: 225 FH 230 H Sbjct: 248 LH 249 >SPAC19G12.15c |tpp1||trehalose-6-phosphate phosphatase Tpp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 36.3 bits (80), Expect = 0.005 Identities = 26/81 (32%), Positives = 36/81 (44%) Frame = +2 Query: 266 WKAYIKINEEFAEKTVHALHLLNKQKKDKNGSPPIVWVHDYHLMLAANWIRQRAEEDEIK 445 W+ Y+K+N FA+K K G + V DY L L IR + ++ I Sbjct: 190 WRDYVKMNHAFADKICEIY---------KPGD--FIIVQDYSLFLVPQLIRNKIDDAVI- 237 Query: 446 CKLAFFLHIPFPPWDIFRLFP 508 F+ H PFP +I R FP Sbjct: 238 ---GFYHHHPFPSSEIARCFP 255 Score = 35.1 bits (77), Expect = 0.011 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = +1 Query: 505 PGSDEVLQGILGCDMVGFHIEDYCLNFIDCCQR 603 P +L+ +LG D +GF Y +FI CC R Sbjct: 255 PRRRAILRSVLGADFIGFEDYSYARHFISCCSR 287 >SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 849 Score = 32.7 bits (71), Expect = 0.058 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = +2 Query: 368 IVWVHDYHLMLAANWIRQRAEEDEIKCKLAFFLHIPFPPWDIFR 499 ++ +HDY L L IR++ + I FFLH PF ++FR Sbjct: 220 LILIHDYSLFLLPRLIRKQLSDAPI----VFFLHAPFCTSEVFR 259 Score = 27.9 bits (59), Expect = 1.7 Identities = 9/33 (27%), Positives = 18/33 (54%) Frame = +1 Query: 517 EVLQGILGCDMVGFHIEDYCLNFIDCCQRNFGL 615 E+L+G+L +++ + Y +F+ C GL Sbjct: 266 EILKGVLASNVIAMQTDSYTRHFLSTCSNVLGL 298 >SPAP27G11.05c |vps41||vacuolar protein sorting-associated protein Vps41|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 32.3 bits (70), Expect = 0.077 Identities = 17/46 (36%), Positives = 27/46 (58%) Frame = -1 Query: 705 LVWHSESVAVLTQMVRISPVSTRRFFLSTRQPKVSLAAVNEIQTVI 568 L W SES V+ +I VS +R ++ PK+SL A+ EI +++ Sbjct: 219 LFWQSESRLVIGWSDQIMIVSIQRSNVANELPKISLQALLEIDSIV 264 >SPAC1952.10c |||conserved fungal protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 25.8 bits (54), Expect = 6.7 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -3 Query: 397 HQMVVVNPHDRWTSIFVFLLFVQQMESVDCLLSKLLVY 284 H+ +VVN H + F++ + + V C+L LVY Sbjct: 141 HERIVVNSHMLLFYLKPFVIALPVLIGVTCILLSFLVY 178 >SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 693 Score = 25.8 bits (54), Expect = 6.7 Identities = 14/37 (37%), Positives = 18/37 (48%) Frame = -1 Query: 231 NETTARMSRYSIHCSSYRKV*VPREWGLFSPNAGQRL 121 N + MSRY H S+Y K V L N+GQ + Sbjct: 320 NNESYEMSRYEKHLSAYEKANVKVASSLAFLNSGQAI 356 >SPBC839.02 |||arrestin Aly1 related|Schizosaccharomyces pombe|chr 2|||Manual Length = 530 Score = 25.4 bits (53), Expect = 8.8 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +3 Query: 168 PKLFDSYYNGCCNGTFWPLFHS 233 PKLFD+Y W FHS Sbjct: 134 PKLFDTYEENSIMNHCWVFFHS 155 >SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 25.4 bits (53), Expect = 8.8 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -3 Query: 559 GIPPYHNPKFPAKLHQTRE 503 G PPY P P+KL TR+ Sbjct: 581 GPPPYVRPSAPSKLPDTRQ 599 >SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 8.8 Identities = 14/50 (28%), Positives = 24/50 (48%) Frame = -1 Query: 663 VRISPVSTRRFFLSTRQPKVSLAAVNEIQTVILYVESHHITTQNSLQNFI 514 V +SP+S S + PK+S +AV + I H ++ + L F+ Sbjct: 316 VPMSPISISPAKPSPQSPKLSQSAVGHPSSSIPAAAMHKVSYSDDLMRFV 365 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,550,510 Number of Sequences: 5004 Number of extensions: 81353 Number of successful extensions: 240 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 218 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 235 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 361294920 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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