BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbpv0071
(599 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g16880.3 68418.m01977 VHS domain-containing protein / GAT dom... 72 3e-13
At5g16880.2 68418.m01979 VHS domain-containing protein / GAT dom... 72 3e-13
At5g16880.1 68418.m01978 VHS domain-containing protein / GAT dom... 72 3e-13
At1g21380.1 68414.m02675 VHS domain-containing protein / GAT dom... 65 3e-11
At2g38410.1 68415.m04718 VHS domain-containing protein / GAT dom... 65 4e-11
At1g76970.1 68414.m08962 VHS domain-containing protein / GAT dom... 60 8e-10
At3g08790.1 68416.m01021 VHS domain-containing protein / GAT dom... 48 4e-06
At5g01760.1 68418.m00095 VHS domain-containing protein / GAT dom... 48 5e-06
At4g32760.1 68417.m04661 VHS domain-containing protein / GAT dom... 45 4e-05
At5g63640.1 68418.m07990 VHS domain-containing protein / GAT dom... 43 1e-04
At1g06210.2 68414.m00653 VHS domain-containing protein / GAT dom... 36 0.020
At1g06210.1 68414.m00654 VHS domain-containing protein / GAT dom... 36 0.020
At3g24710.1 68416.m03102 hypothetical protein 29 3.1
At3g62270.1 68416.m06996 anion exchange family protein contains ... 28 5.4
At5g39490.1 68418.m04782 F-box family protein contains Pfam:PF00... 27 9.5
At3g59290.1 68416.m06609 epsin N-terminal homology (ENTH) domain... 27 9.5
At1g11960.1 68414.m01382 early-responsive to dehydration protein... 27 9.5
>At5g16880.3 68418.m01977 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate [Homo
sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
domain, PF03127: GAT domain
Length = 297
Score = 72.1 bits (169), Expect = 3e-13
Identities = 38/96 (39%), Positives = 55/96 (57%), Gaps = 2/96 (2%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVV--QDKVLSLIQCWADAFQNQAEL 429
TCVKNC K F + + + E+VKLI +DP TVV ++K L LI+ W ++ L
Sbjct: 110 TCVKNCEKAFSEVAAER-VLDEMVKLI---DDPQTVVNNRNKALMLIEAWGESTSELRYL 165
Query: 430 QGVGQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVP 537
+ Y L+ +G+ FP D +++ PIFTP RS P
Sbjct: 166 PVFEETYKSLKARGIRFPGRDNESLAPIFTPARSTP 201
Score = 43.2 bits (97), Expect = 1e-04
Identities = 18/47 (38%), Positives = 28/47 (59%)
Frame = +2
Query: 59 PVGQKIEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRL 199
P + +E AT L +W +N+EICD+IN T + I+ I+KR+
Sbjct: 47 PTDKIVEDATTENLEEPDWDMNLEICDMINQETINSVELIRGIKKRI 93
>At5g16880.2 68418.m01979 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate [Homo
sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
domain, PF03127: GAT domain
Length = 407
Score = 72.1 bits (169), Expect = 3e-13
Identities = 38/96 (39%), Positives = 55/96 (57%), Gaps = 2/96 (2%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVV--QDKVLSLIQCWADAFQNQAEL 429
TCVKNC K F + + + E+VKLI +DP TVV ++K L LI+ W ++ L
Sbjct: 110 TCVKNCEKAFSEVAAER-VLDEMVKLI---DDPQTVVNNRNKALMLIEAWGESTSELRYL 165
Query: 430 QGVGQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVP 537
+ Y L+ +G+ FP D +++ PIFTP RS P
Sbjct: 166 PVFEETYKSLKARGIRFPGRDNESLAPIFTPARSTP 201
Score = 43.2 bits (97), Expect = 1e-04
Identities = 18/47 (38%), Positives = 28/47 (59%)
Frame = +2
Query: 59 PVGQKIEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRL 199
P + +E AT L +W +N+EICD+IN T + I+ I+KR+
Sbjct: 47 PTDKIVEDATTENLEEPDWDMNLEICDMINQETINSVELIRGIKKRI 93
>At5g16880.1 68418.m01978 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate [Homo
sapiens] GI:2731383; contains Pfam profiles PF00790: VHS
domain, PF03127: GAT domain
Length = 407
Score = 72.1 bits (169), Expect = 3e-13
Identities = 38/96 (39%), Positives = 55/96 (57%), Gaps = 2/96 (2%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVV--QDKVLSLIQCWADAFQNQAEL 429
TCVKNC K F + + + E+VKLI +DP TVV ++K L LI+ W ++ L
Sbjct: 110 TCVKNCEKAFSEVAAER-VLDEMVKLI---DDPQTVVNNRNKALMLIEAWGESTSELRYL 165
Query: 430 QGVGQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVP 537
+ Y L+ +G+ FP D +++ PIFTP RS P
Sbjct: 166 PVFEETYKSLKARGIRFPGRDNESLAPIFTPARSTP 201
Score = 43.2 bits (97), Expect = 1e-04
Identities = 18/47 (38%), Positives = 28/47 (59%)
Frame = +2
Query: 59 PVGQKIEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRL 199
P + +E AT L +W +N+EICD+IN T + I+ I+KR+
Sbjct: 47 PTDKIVEDATTENLEEPDWDMNLEICDMINQETINSVELIRGIKKRI 93
>At1g21380.1 68414.m02675 VHS domain-containing protein / GAT
domain-containing protein weak similarity to Hrs [Rattus
norvegicus] GI:8547026; contains Pfam profiles PF00790:
VHS domain, PF03127: GAT domain
Length = 506
Score = 65.3 bits (152), Expect = 3e-11
Identities = 32/95 (33%), Positives = 54/95 (56%), Gaps = 1/95 (1%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQNQ-AELQ 432
T KNCG+ + L+ +++ + ++VK++ K P V++K+LSL+ W +AF
Sbjct: 67 TLSKNCGESVYQLIVDRDILPDMVKIV--KKKPDLTVREKILSLLDTWQEAFGGSGGRFP 124
Query: 433 GVGQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVP 537
YNELR+ G+EFP ++ P FTP ++ P
Sbjct: 125 QYYNAYNELRSAGIEFP-PRTESSVPFFTPPQTQP 158
Score = 52.8 bits (121), Expect = 2e-07
Identities = 23/59 (38%), Positives = 36/59 (61%)
Frame = +2
Query: 77 EQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
E+AT+ L +WA+N+E+CDIIN K+A+K ++KRL + K + +Y L L
Sbjct: 10 ERATNDMLIGPDWAINIELCDIINMEPSQAKEAVKVLKKRLGSKNSKVQILALYALETL 68
>At2g38410.1 68415.m04718 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 671
Score = 64.9 bits (151), Expect = 4e-11
Identities = 39/99 (39%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQN-QAELQ 432
T VKNCG H V K + E+VK++ K D V+DK+L ++ W AF + +
Sbjct: 70 TLVKNCGDYLHHQVAEKNILGEMVKIVKKKAD--MQVRDKILVMVDSWQQAFGGPEGKYP 127
Query: 433 GVGQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVPDGRR 549
Y+ELR GVEFP DA PI TP S P R+
Sbjct: 128 QYYWAYDELRRSGVEFPRRSPDA-SPIITPPVSHPPLRQ 165
Score = 47.6 bits (108), Expect = 6e-06
Identities = 25/61 (40%), Positives = 36/61 (59%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
+++AT L +W NMEICD +NS KD +KA++KRL K+ V + LT+L
Sbjct: 12 VDKATSDLLLGPDWTTNMEICDSVNSLHWQAKDVVKAVKKRL---QHKSSRVQLLALTLL 68
Query: 254 E 256
E
Sbjct: 69 E 69
>At1g76970.1 68414.m08962 VHS domain-containing protein / GAT
domain-containing protein weak similarity to
HGF-regulated tyrosine kinase substrate [Mus musculus]
GI:1089781; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 446
Score = 60.5 bits (140), Expect = 8e-10
Identities = 30/96 (31%), Positives = 55/96 (57%), Gaps = 1/96 (1%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQNQ-AELQ 432
T KNCG+ + L+ ++ ++++VK++ K P V++K+L+L+ W +AF +
Sbjct: 67 TLSKNCGENVYQLIIDRGLLNDMVKIV--KKKPELNVREKILTLLDTWQEAFGGRGGRYP 124
Query: 433 GVGQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVPD 540
YN+LR+ G+EFP ++ FTP ++ PD
Sbjct: 125 QYYNAYNDLRSAGIEFPPRTESSLS-FFTPPQTQPD 159
Score = 52.0 bits (119), Expect = 3e-07
Identities = 22/59 (37%), Positives = 36/59 (61%)
Frame = +2
Query: 77 EQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
E+AT+ L +WA+N+E+CD+IN K+A+K ++KRL + K + +Y L L
Sbjct: 10 ERATNDMLIGPDWAINIELCDLINMDPSQAKEAVKVLKKRLGSKNSKVQILALYALETL 68
>At3g08790.1 68416.m01021 VHS domain-containing protein / GAT
domain-containing protein weak similarity to
HGF-regulated tyrosine kinase substrate [Mus musculus]
GI:1089781; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 607
Score = 48.4 bits (110), Expect = 4e-06
Identities = 22/61 (36%), Positives = 37/61 (60%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
+++AT L +WA+N+EICD++N ++ + I+KRLT+ K V + LT+L
Sbjct: 6 VDRATSDMLIGPDWAMNLEICDMLNHEPGQTREVVSGIKKRLTSRTSK---VQLLALTLL 62
Query: 254 E 256
E
Sbjct: 63 E 63
Score = 47.6 bits (108), Expect = 6e-06
Identities = 25/77 (32%), Positives = 39/77 (50%), Gaps = 1/77 (1%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQN-QAELQ 432
T + NCG+ H+ V K+ + ++VK+ K P V++K+L LI W ++F Q
Sbjct: 64 TIITNCGELIHMQVAEKDILHKMVKMA--KRKPNIQVKEKILILIDTWQESFSGPQGRHP 121
Query: 433 GVGQVYNELRTKGVEFP 483
Y EL G+ FP
Sbjct: 122 QYYAAYQELLRAGIVFP 138
>At5g01760.1 68418.m00095 VHS domain-containing protein / GAT
domain-containing protein weak similarity to Hrs [Rattus
norvegicus] GI:8547026; contains Pfam profiles PF00790:
VHS domain, PF03127: GAT domain
Length = 542
Score = 48.0 bits (109), Expect = 5e-06
Identities = 27/81 (33%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
Frame = +1
Query: 262 VKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQNQA-ELQGV 438
+KNCG H + K + ++VKL+ K D V++K+L L+ W +AF A +
Sbjct: 86 LKNCGDFVHSHIAEKHLLEDMVKLVRKKGD--FEVRNKLLILLDTWNEAFSGVACKHPHY 143
Query: 439 GQVYNELRTKGVEFPMTDLDA 501
Y EL+ GV+FP +A
Sbjct: 144 NWAYQELKRCGVKFPQRSKEA 164
Score = 44.8 bits (101), Expect = 4e-05
Identities = 24/61 (39%), Positives = 39/61 (63%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
+++AT L + +W + + ICD +NS+ KDAIKA+++RL K+ V + TLT+L
Sbjct: 26 VDKATSELLRTPDWTIIIAICDSLNSNRWQCKDAIKAVKRRL---QHKSSRVQLLTLTLL 82
Query: 254 E 256
E
Sbjct: 83 E 83
>At4g32760.1 68417.m04661 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 838
Score = 44.8 bits (101), Expect = 4e-05
Identities = 22/52 (42%), Positives = 31/52 (59%)
Frame = +1
Query: 256 TCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAF 411
T VKNCG H+ V K I E+V+++ K P V++K+L LI W +AF
Sbjct: 80 TIVKNCGDMVHMHVAEKGVIHEMVRIV--KKKPDFHVKEKILVLIDTWQEAF 129
Score = 36.3 bits (80), Expect = 0.015
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +2
Query: 62 VGQKIEQATDGALPSENWALNMEICDIINS 151
V +E+AT L +WA+N+EICD++NS
Sbjct: 2 VNAMVERATSEMLIGPDWAMNLEICDMLNS 31
>At5g63640.1 68418.m07990 VHS domain-containing protein / GAT
domain-containing protein weak similarity to hepatocyte
growth factor-regulated tyrosine kinase substrate HRS
isoform 2 [Homo sapiens] GI:9022389; contains Pfam
profiles PF00790: VHS domain, PF03127: GAT domain
Length = 447
Score = 43.2 bits (97), Expect = 1e-04
Identities = 23/61 (37%), Positives = 32/61 (52%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
+ AT L +WA N+EIC++ KD IKAI+KRL KN +Y + +L
Sbjct: 6 VSSATSEKLADVDWAKNIEICELAARDERQAKDVIKAIKKRL---GSKNPNTQLYAVQLL 62
Query: 254 E 256
E
Sbjct: 63 E 63
Score = 27.1 bits (57), Expect = 9.5
Identities = 22/93 (23%), Positives = 40/93 (43%), Gaps = 1/93 (1%)
Frame = +1
Query: 262 VKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQN-QAELQGV 438
+ N G+ H V + + LVK++ K+D P V++++ L+ + +
Sbjct: 66 MNNIGENIHKQVIDTGVLPTLVKIVKKKSDLP--VRERIFLLLDATQTSLGGASGKFPQY 123
Query: 439 GQVYNELRTKGVEFPMTDLDAMGPIFTPQRSVP 537
Y EL GV+F T P+ ++VP
Sbjct: 124 YTAYYELVNAGVKF--TQRPNATPVVVTAQAVP 154
>At1g06210.2 68414.m00653 VHS domain-containing protein / GAT
domain-containing protein weak similarity to SP|Q9UJY5
ADP-ribosylation factor binding protein GGA1 {Homo
sapiens}; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 279
Score = 35.9 bits (79), Expect = 0.020
Identities = 18/61 (29%), Positives = 34/61 (55%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
+++AT L NW +NM IC IN+ + ++AI++++ +GK+ +L +L
Sbjct: 42 VDEATLETLEEPNWGMNMRICAQINNDEFNGTEIVRAIKRKI---SGKSPVSQRLSLELL 98
Query: 254 E 256
E
Sbjct: 99 E 99
>At1g06210.1 68414.m00654 VHS domain-containing protein / GAT
domain-containing protein weak similarity to SP|Q9UJY5
ADP-ribosylation factor binding protein GGA1 {Homo
sapiens}; contains Pfam profiles PF00790: VHS domain,
PF03127: GAT domain
Length = 383
Score = 35.9 bits (79), Expect = 0.020
Identities = 18/61 (29%), Positives = 34/61 (55%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVL 253
+++AT L NW +NM IC IN+ + ++AI++++ +GK+ +L +L
Sbjct: 42 VDEATLETLEEPNWGMNMRICAQINNDEFNGTEIVRAIKRKI---SGKSPVSQRLSLELL 98
Query: 254 E 256
E
Sbjct: 99 E 99
>At3g24710.1 68416.m03102 hypothetical protein
Length = 126
Score = 28.7 bits (61), Expect = 3.1
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = +2
Query: 140 IINSSTDGPKDAIKAIRKRLTTSAGKNYTVVMYTLTVLEHA*KTVESRSMFSSAIRNLY 316
+ SST+ P D + A+ + ++ G + V L LE A + S M+ S I+ Y
Sbjct: 17 VAGSSTEMPSDVMSALGEIVSEETGGSKEVASRRLKALEKAQSQLPSCVMYESKIKIKY 75
>At3g62270.1 68416.m06996 anion exchange family protein contains
similarity to anion exchanger 3, cardiac splice form -
Rattus norvegicus, PIR:A42497
Length = 703
Score = 27.9 bits (59), Expect = 5.4
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +2
Query: 65 GQKIEQATDGALPSENWALNMEICDIINSSTDG 163
G+++E++TDG L + + IC II+S G
Sbjct: 55 GEQLERSTDGVLTAVQTLASTAICGIIHSIIGG 87
>At5g39490.1 68418.m04782 F-box family protein contains Pfam:PF00646
F-box domain ; similar to SKP1 interacting partner 2
(SKIP2) TIGR_Ath1:At5g67250
Length = 566
Score = 27.1 bits (57), Expect = 9.5
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = -1
Query: 593 RVLRQDGSXEGNQLVRRPSGTLRCGVKI 510
RVL EGN+LV RP+GT VKI
Sbjct: 428 RVLIGTKILEGNRLVSRPNGTTMFVVKI 455
>At3g59290.1 68416.m06609 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain;
similar to Af10-protein - Avena fatua, EMBL:U80041
Length = 1024
Score = 27.1 bits (57), Expect = 9.5
Identities = 23/65 (35%), Positives = 35/65 (53%), Gaps = 3/65 (4%)
Frame = +2
Query: 74 IEQATDGALPSENWALNMEI-CDIINSSTDGPKDAIK--AIRKRLTTSAGKNYTVVMYTL 244
IEQ A +E+W + + DI ++S + + I + KRL+ S GKN+ V L
Sbjct: 26 IEQKVLDATSNESWGPHGSLLADIAHASRNYHEYQITMGVLWKRLSDS-GKNWRHVYKAL 84
Query: 245 TVLEH 259
TVLE+
Sbjct: 85 TVLEY 89
>At1g11960.1 68414.m01382 early-responsive to dehydration
protein-related / ERD protein-related low similarity to
ERD4 protein [Arabidopsis thaliana] GI:15375406
Length = 375
Score = 27.1 bits (57), Expect = 9.5
Identities = 18/57 (31%), Positives = 27/57 (47%)
Frame = -1
Query: 392 WMRLNTLSCTTVGGSFLGPINFTNSDINSLLQTRTWNGFPQFFTHVPGQSACTSPLC 222
W L ++ T+ G N T+SDI+ L + NG +F+TH+ A T C
Sbjct: 117 WSILVPVNWTSHGLQLAKLRNVTSSDIDKLSISNIENGSDRFWTHLVMAYAFTFWTC 173
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,276,782
Number of Sequences: 28952
Number of extensions: 355950
Number of successful extensions: 926
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 918
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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