Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVm1458
         (803 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   144   1e-35
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   144   1e-35
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   144   1e-35
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    52   1e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    35   0.012
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.11 
SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding...    27   3.1  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    27   4.1  
SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    26   7.2  
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    26   7.2  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   9.5  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  144 bits (350), Expect = 1e-35
 Identities = 65/84 (77%), Positives = 73/84 (86%)
 Frame = -3

Query: 759 IVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKG 580
           IV FAPA +TTEVKSVEMHHE+L   +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G
Sbjct: 275 IVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMG 334

Query: 579 AADFTAQVIVLNHPGQISNGYTPV 508
            A FTAQVI+LNHPGQIS GY+PV
Sbjct: 335 CASFTAQVIILNHPGQISAGYSPV 358



 Score =  137 bits (331), Expect = 2e-33
 Identities = 61/81 (75%), Positives = 71/81 (87%)
 Frame = -2

Query: 508 LDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPP 329
           LDCHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ P
Sbjct: 359 LDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418

Query: 328 LGRFAVRDMRQTVAVGVIKAV 266
           LGRFAVRDMRQTVAVGVIKAV
Sbjct: 419 LGRFAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  144 bits (350), Expect = 1e-35
 Identities = 65/84 (77%), Positives = 73/84 (86%)
 Frame = -3

Query: 759 IVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKG 580
           IV FAPA +TTEVKSVEMHHE+L   +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G
Sbjct: 275 IVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMG 334

Query: 579 AADFTAQVIVLNHPGQISNGYTPV 508
            A FTAQVI+LNHPGQIS GY+PV
Sbjct: 335 CASFTAQVIILNHPGQISAGYSPV 358



 Score =  137 bits (331), Expect = 2e-33
 Identities = 61/81 (75%), Positives = 71/81 (87%)
 Frame = -2

Query: 508 LDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPP 329
           LDCHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ P
Sbjct: 359 LDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418

Query: 328 LGRFAVRDMRQTVAVGVIKAV 266
           LGRFAVRDMRQTVAVGVIKAV
Sbjct: 419 LGRFAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  144 bits (350), Expect = 1e-35
 Identities = 65/84 (77%), Positives = 73/84 (86%)
 Frame = -3

Query: 759 IVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKG 580
           IV FAPA +TTEVKSVEMHHE+L   +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G
Sbjct: 275 IVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMG 334

Query: 579 AADFTAQVIVLNHPGQISNGYTPV 508
            A FTAQVI+LNHPGQIS GY+PV
Sbjct: 335 CASFTAQVIILNHPGQISAGYSPV 358



 Score =  137 bits (331), Expect = 2e-33
 Identities = 61/81 (75%), Positives = 71/81 (87%)
 Frame = -2

Query: 508 LDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPP 329
           LDCHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ P
Sbjct: 359 LDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAP 418

Query: 328 LGRFAVRDMRQTVAVGVIKAV 266
           LGRFAVRDMRQTVAVGVIKAV
Sbjct: 419 LGRFAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 52.0 bits (119), Expect = 1e-07
 Identities = 28/80 (35%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
 Frame = -2

Query: 499 HTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 320
           HTA     FA++  K+D+ T + ++  P     G   I  L    P+C+E F+++  +GR
Sbjct: 584 HTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGR 642

Query: 319 FAVRDMRQTVAVG-VIKAVN 263
           F +RD   TVAVG V+K ++
Sbjct: 643 FTLRDQGTTVAVGKVVKILD 662



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 25/84 (29%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
 Frame = -3

Query: 756 VVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKG 580
           V+  P N T EV ++ +   E +  ++ GD V   V+     +++ GYV   +KN P   
Sbjct: 499 VLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHA 556

Query: 579 AADFTAQVIVLNHPGQISNGYTPV 508
              F AQ+ +L  P  ++ GY+ V
Sbjct: 557 TTRFIAQIAILELPSILTTGYSCV 580


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 35.1 bits (77), Expect = 0.012
 Identities = 14/43 (32%), Positives = 27/43 (62%)
 Frame = -3

Query: 741 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 613
           +++ T V  +EM  + L  AV GDN G  ++++  ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.11
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = -2

Query: 364 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 272
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding
           protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 279

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = +1

Query: 679 YSFLESFVVHLHRLDFSSDVGGGKDNNGPGFNT 777
           + FLE+  VHLH    ++ V G    N PG+ T
Sbjct: 158 FHFLEAIAVHLHFTQKANAVNGA---NAPGYGT 187


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
           Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +1

Query: 628 QFLDGHVLYVETYIVSRYSFLESFV 702
           +F DGH+L  ETY+      LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551


>SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 325

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 19/51 (37%), Positives = 24/51 (47%)
 Frame = +3

Query: 375 TKLTMAASPDLMDFGLTSVDLPVRRSTFSLISANLQAMWAVWQSKLVCNRL 527
           T L+ AA P L D   TSV   +  +  SL SA   A    W+  +VC  L
Sbjct: 256 TSLSEAALPPLTDVQRTSVLSLLTSTLHSLESARQIADSNTWKPIIVCLEL 306


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 2/32 (6%)
 Frame = +2

Query: 686 SWRASWCISTDLTSVV--MLAGAKTTMGLVST 775
           SW  SW +ST L  +V  M +   TT G+V++
Sbjct: 97  SWNPSWMVSTILVGLVSFMTSDEITTGGIVTS 128


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = +3

Query: 387 MAASPDLMDFGLTSVDLPVRRSTFS 461
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,267,640
Number of Sequences: 5004
Number of extensions: 67115
Number of successful extensions: 189
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)