BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm1339 (650 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g37600.1 68418.m04529 glutamine synthetase, putative similar ... 107 6e-24 At1g66200.1 68414.m07514 glutamine synthetase, putative similar ... 105 3e-23 At5g16570.1 68418.m01939 glutamine synthetase, putative similar ... 104 4e-23 At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to... 103 1e-22 At1g48470.1 68414.m05418 glutamine synthetase, putative similar ... 103 1e-22 At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to... 102 2e-22 At3g52010.1 68416.m05705 serine carboxypeptidase S10 family prot... 29 3.5 At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to e... 29 3.5 At1g66520.1 68414.m07558 formyltransferase, putative similar to ... 29 3.5 At2g35600.1 68415.m06030 expressed protein 28 4.7 At5g49270.1 68418.m06098 phytochelatin synthetase-related contai... 28 6.2 At4g32880.1 68417.m04679 homeobox-leucine zipper transcription f... 28 6.2 At1g79120.1 68414.m09226 hypothetical protein 28 6.2 >At5g37600.1 68418.m04529 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (Glutamate-- ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899 Length = 356 Score = 107 bits (257), Expect = 6e-24 Identities = 49/83 (59%), Positives = 58/83 (69%) Frame = +1 Query: 250 STPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 429 S DKI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+ L Sbjct: 13 SDSTDKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGEDSEVIL 72 Query: 430 VPRAIYKDPFRRGNHILVMCDTY 498 P+AI+KDPFRRGN+ILVMCD Y Sbjct: 73 YPQAIFKDPFRRGNNILVMCDAY 95 Score = 41.9 bits (94), Expect = 4e-04 Identities = 19/47 (40%), Positives = 27/47 (57%), Gaps = 3/47 (6%) Frame = +3 Query: 510 EPTESNNRISCQEAYDK--CKDDEPWFGIEQEYILLDSDLR-PFGWP 641 EP +N R + + + + PW+GIEQEY LL D++ P GWP Sbjct: 100 EPIPTNKRHAAAKVFSNPDVAAEVPWYGIEQEYTLLQKDVKWPVGWP 146 >At1g66200.1 68414.m07514 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (Glutamate-- ammonia ligase, GS1) [Lotus japonicus] SWISS-PROT:Q42899 Length = 356 Score = 105 bits (252), Expect = 3e-23 Identities = 48/83 (57%), Positives = 59/83 (71%) Frame = +1 Query: 250 STPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 429 S ++KI+A YIW+ GSG +R K RTL P LP WN+DGSST QA G +S+ L Sbjct: 13 SDNSEKIIAEYIWVGGSGMDMRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGQDSEVIL 72 Query: 430 VPRAIYKDPFRRGNHILVMCDTY 498 P+AI+KDPFRRGN+ILVMCD Y Sbjct: 73 YPQAIFKDPFRRGNNILVMCDAY 95 Score = 41.1 bits (92), Expect = 6e-04 Identities = 20/47 (42%), Positives = 26/47 (55%), Gaps = 3/47 (6%) Frame = +3 Query: 510 EPTESNNRISCQEAYDKCK--DDEPWFGIEQEYILLDSDLR-PFGWP 641 EP +N R + E + + PW+GIEQEY LL D+ P GWP Sbjct: 100 EPIPTNKRHAAAEIFANPDVIAEVPWYGIEQEYTLLQKDVNWPLGWP 146 >At5g16570.1 68418.m01939 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (glutamate-- ammonia ligase) [Alfalfa] SWISS-PROT:P04078 Length = 356 Score = 104 bits (250), Expect = 4e-23 Identities = 48/83 (57%), Positives = 58/83 (69%) Frame = +1 Query: 250 STPADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYL 429 S D+I+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+ + Sbjct: 13 SDSTDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEVII 72 Query: 430 VPRAIYKDPFRRGNHILVMCDTY 498 P+AI+KDPFRRGN+ILVMCD Y Sbjct: 73 YPQAIFKDPFRRGNNILVMCDAY 95 Score = 41.9 bits (94), Expect = 4e-04 Identities = 19/47 (40%), Positives = 28/47 (59%), Gaps = 3/47 (6%) Frame = +3 Query: 510 EPTESNNRISCQEAYD--KCKDDEPWFGIEQEYILLDSDLR-PFGWP 641 EP +N R + + ++ +E W+GIEQEY LL D++ P GWP Sbjct: 100 EPIPTNKRHAAAKIFEDPSVVAEETWYGIEQEYTLLQKDIKWPVGWP 146 >At5g35630.1 68418.m04253 glutamine synthetase (GS2) identical to glutamine synthetase, chloroplast precursor (glutamate-- ammonia ligase, GS2) [Arabidopsis thaliana] SWISS-PROT:Q43127 Length = 430 Score = 103 bits (247), Expect = 1e-22 Identities = 45/80 (56%), Positives = 60/80 (75%) Frame = +1 Query: 259 ADKILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPR 438 +D+I+A YIWI GSG LR K RT+ + P +LP WN+DGSST QA G +S+ L P+ Sbjct: 74 SDRIIAEYIWIGGSGIDLRSKSRTIEKPVEDPSELPKWNYDGSSTGQAPGEDSEVILYPQ 133 Query: 439 AIYKDPFRRGNHILVMCDTY 498 AI++DPFR GN+ILV+CDT+ Sbjct: 134 AIFRDPFRGGNNILVICDTW 153 Score = 46.0 bits (104), Expect = 2e-05 Identities = 21/47 (44%), Positives = 27/47 (57%), Gaps = 3/47 (6%) Frame = +3 Query: 510 EPTESNNRISCQEAYD--KCKDDEPWFGIEQEYILLDSDLR-PFGWP 641 EP +N R E + K + PWFGIEQEY LL +++ P GWP Sbjct: 158 EPIPTNKRAKAAEIFSNKKVSGEVPWFGIEQEYTLLQQNVKWPLGWP 204 >At1g48470.1 68414.m05418 glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (glutamate-- ammonia ligase, GS1) [Arabidopsis thaliana] SWISS-PROT:Q9LVI8 Length = 353 Score = 103 bits (247), Expect = 1e-22 Identities = 47/79 (59%), Positives = 58/79 (73%) Frame = +1 Query: 265 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 444 KI+A YIWI GSG +R K RTL P LP WN+DGSST+QA G +S+ L P+AI Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVSNPTKLPKWNYDGSSTDQAAGDDSEVILYPQAI 77 Query: 445 YKDPFRRGNHILVMCDTYK 501 +KDPFR+GN+ILVMCD Y+ Sbjct: 78 FKDPFRKGNNILVMCDAYR 96 Score = 50.8 bits (116), Expect = 8e-07 Identities = 23/47 (48%), Positives = 30/47 (63%), Gaps = 3/47 (6%) Frame = +3 Query: 510 EPTESNNRISCQEAYD--KCKDDEPWFGIEQEYILLDSDLR-PFGWP 641 +P +NNR + +D K +EPWFGIEQEY LL D++ P GWP Sbjct: 100 DPIPTNNRHKAVKIFDHPNVKAEEPWFGIEQEYTLLKKDVKWPLGWP 146 >At3g17820.1 68416.m02272 glutamine synthetase (GS1) identical to glutamine synthetase, cytosolic isozyme (glutamate-- ammonia ligase, GS1) [Arabidopsis thaliana] SWISS-PROT:Q9LVI8 Length = 354 Score = 102 bits (245), Expect = 2e-22 Identities = 47/78 (60%), Positives = 56/78 (71%) Frame = +1 Query: 265 KILATYIWIDGSGEHLRCKDRTLNFIPKAPKDLPIWNFDGSSTNQADGHNSDTYLVPRAI 444 KI+A YIWI GSG +R K RTL P LP WN+DGSST QA G +S+ L P+AI Sbjct: 18 KIIAEYIWIGGSGMDIRSKARTLPGPVTDPSKLPKWNYDGSSTGQAAGEDSEVILYPQAI 77 Query: 445 YKDPFRRGNHILVMCDTY 498 +KDPFR+GN+ILVMCD Y Sbjct: 78 FKDPFRKGNNILVMCDAY 95 Score = 41.9 bits (94), Expect = 4e-04 Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 1/44 (2%) Frame = +3 Query: 513 PTESNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLR-PFGWP 641 PT + + ++ +EPW+GIEQEY L+ D+ P GWP Sbjct: 103 PTNKRHNAAKIFSHPDVAKEEPWYGIEQEYTLMQKDVNWPIGWP 146 >At3g52010.1 68416.m05705 serine carboxypeptidase S10 family protein similar to SP|P52711 Serine carboxypeptidase II-3 precursor (EC 3.4.16.6) Hordeum vulgare; contains Pfam profile PF0450 serine carboxypeptidase Length = 487 Score = 28.7 bits (61), Expect = 3.5 Identities = 10/30 (33%), Positives = 20/30 (66%) Frame = -3 Query: 99 KCSLDASVEYCAKTYLRKVLSERKMFRSLH 10 KC+ V+ C+K YL+ L+ +K+ +++H Sbjct: 326 KCTTVLEVDPCSKDYLKAYLNRKKVQKAIH 355 >At3g15370.1 68416.m01949 expansin, putative (EXP12) similar to expansin GI:11191999 from [Lycopersicon esculentum]; alpha-expansin gene family, PMID:11641069 Length = 252 Score = 28.7 bits (61), Expect = 3.5 Identities = 16/49 (32%), Positives = 23/49 (46%) Frame = -3 Query: 465 STEWILVNSTRYEVGIRIVPISLVG*AAIEVPYRQIFGSFRYEIQGAIF 319 S WI ++T Y G+ P SL G + PY FG+ + G +F Sbjct: 24 SNGWIRAHATYY--GVNDSPASLGGACGYDNPYHAGFGAHTAALSGELF 70 >At1g66520.1 68414.m07558 formyltransferase, putative similar to methionyl-tRNA formyltransferase [strain N315- Staphylococcus aureus] SWISS-PROT:Q99UQ2 Length = 355 Score = 28.7 bits (61), Expect = 3.5 Identities = 12/29 (41%), Positives = 16/29 (55%), Gaps = 1/29 (3%) Frame = +3 Query: 558 KCKDDEPWFGIEQEYILLDSDLRPF-GWP 641 K DE W +QE +L + +R F GWP Sbjct: 238 KIAPDEAWLSFDQEAFVLHNKVRAFAGWP 266 >At2g35600.1 68415.m06030 expressed protein Length = 331 Score = 28.3 bits (60), Expect = 4.7 Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 3/66 (4%) Frame = +2 Query: 110 SCLTCFKMADTAHTKIEDNPKILSGPVLTNSPNAVLSKTLLSRYNDLPRRLT---RFSPP 280 +C+ C KMAD ED + G N+ AV K+L ++ D+ + + + S P Sbjct: 3 TCINCTKMADRGEEDEEDEAR---GSTTPNTKEAV--KSLTTQIKDMASKFSGSHKQSKP 57 Query: 281 TFGSTA 298 T GS++ Sbjct: 58 TPGSSS 63 >At5g49270.1 68418.m06098 phytochelatin synthetase-related contains Pfam PF04833: Phytochelatin synthetase-like conserved region Length = 663 Score = 27.9 bits (59), Expect = 6.2 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = -3 Query: 504 VFIRITHNEDVISSTEWILVNST 436 VF+ HNE +IS+T+ ++VN T Sbjct: 91 VFVGFQHNEILISATDALIVNGT 113 >At4g32880.1 68417.m04679 homeobox-leucine zipper transcription factor (HB-8) identical to HD-zip transcription factor (athb-8) (GI:7270235) [Arabidopsis thaliana] Length = 833 Score = 27.9 bits (59), Expect = 6.2 Identities = 14/34 (41%), Positives = 17/34 (50%) Frame = +3 Query: 282 HLDRRLWRTPEVQRSHLEFHT*SSQRSAYMELRW 383 H D W PEV RS E T +QR+ LR+ Sbjct: 332 HFDLEPWSVPEVLRSLYESSTLLAQRTTMAALRY 365 >At1g79120.1 68414.m09226 hypothetical protein Length = 413 Score = 27.9 bits (59), Expect = 6.2 Identities = 15/49 (30%), Positives = 25/49 (51%) Frame = +1 Query: 502 YNRSQQRATIASVVKKLTTNARMMSPGLG*NRNIFYWIQICAPSGGPGW 648 Y RS + I + K T + ++ PG +R + ++Q+ SGG GW Sbjct: 7 YRRSSIFSVILRIHKLRETESFVLFPGESSSRLQWTFVQVRHKSGGGGW 55 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,279,610 Number of Sequences: 28952 Number of extensions: 329567 Number of successful extensions: 795 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 775 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 795 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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