Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm1328
(735 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 98 6e-21
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 94 8e-20
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 94 8e-20
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 94 8e-20
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 93 2e-19
At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplas... 27 9.8
At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplas... 27 9.8
At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplas... 27 9.8
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 97.9 bits (233), Expect = 6e-21
Identities = 45/82 (54%), Positives = 62/82 (75%), Gaps = 2/82 (2%)
Frame = +2
Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
V++ GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++QQSP+IA GVH +
Sbjct: 51 VMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGH 110
Query: 434 --RNDEEVGAGDQGLMFGYATD 493
+ EEVGAGDQG MFGYATD
Sbjct: 111 LTKKPEEVGAGDQGHMFGYATD 132
Score = 88.2 bits (209), Expect = 5e-18
Identities = 43/70 (61%), Positives = 50/70 (71%), Gaps = 4/70 (5%)
Frame = +3
Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCF----CVAKSHP 281
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT M K++
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 282 KLTWIIKKLC 311
I++K C
Sbjct: 64 DYEQIVRKTC 73
Score = 57.2 bits (132), Expect = 1e-08
Identities = 26/46 (56%), Positives = 32/46 (69%)
Frame = +1
Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGA 642
++PLT VLA KL K+ E+R+NG W RPD KTQVT EY+ GA
Sbjct: 137 LMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGA 182
Score = 34.3 bits (75), Expect = 0.085
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +3
Query: 645 VPQRVHTVVVSLQHSEKITLETLRDEIRE 731
VP RVHTV++S QH E +T + + +++E
Sbjct: 184 VPVRVHTVLISTQHDETVTNDEIAADLKE 212
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 94.3 bits (224), Expect = 8e-20
Identities = 42/82 (51%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
Frame = +2
Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
V++ GEIT+KA +DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH +
Sbjct: 51 VMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110
Query: 434 --RNDEEVGAGDQGLMFGYATD 493
+ E++GAGDQG MFGYATD
Sbjct: 111 FTKRPEDIGAGDQGHMFGYATD 132
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/65 (64%), Positives = 48/65 (73%)
Frame = +3
Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M + K T
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63
Query: 294 IIKKL 308
+K+
Sbjct: 64 DYEKI 68
Score = 52.0 bits (119), Expect = 4e-07
Identities = 23/46 (50%), Positives = 31/46 (67%)
Frame = +1
Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGA 642
++PL+ VLA K+ ++ E+R+NG W RPD KTQVT EY GA
Sbjct: 137 LMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGA 182
Score = 34.3 bits (75), Expect = 0.085
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +3
Query: 645 VPQRVHTVVVSLQHSEKITLETLRDEIRE 731
VP RVHTV++S QH E +T + + +++E
Sbjct: 184 VPVRVHTVLISTQHDETVTNDEIARDLKE 212
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 94.3 bits (224), Expect = 8e-20
Identities = 44/82 (53%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
Frame = +2
Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
V++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH +
Sbjct: 51 VMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110
Query: 434 --RNDEEVGAGDQGLMFGYATD 493
+ EE+GAGDQG MFGYATD
Sbjct: 111 FTKCPEEIGAGDQGHMFGYATD 132
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/65 (64%), Positives = 48/65 (73%)
Frame = +3
Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M + K T
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 294 IIKKL 308
+K+
Sbjct: 64 DYEKI 68
Score = 52.0 bits (119), Expect = 4e-07
Identities = 24/46 (52%), Positives = 31/46 (67%)
Frame = +1
Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGA 642
++PL+ VLA KL ++ E+R+NG W RPD KTQVT EY GA
Sbjct: 137 LMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGA 182
Score = 33.9 bits (74), Expect = 0.11
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +3
Query: 645 VPQRVHTVVVSLQHSEKITLETLRDEIRE 731
VP RVHTV++S QH E +T + + +++E
Sbjct: 184 VPIRVHTVLISTQHDETVTNDEIARDLKE 212
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 94.3 bits (224), Expect = 8e-20
Identities = 44/82 (53%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
Frame = +2
Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
V++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH +
Sbjct: 51 VMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGH 110
Query: 434 --RNDEEVGAGDQGLMFGYATD 493
+ EE+GAGDQG MFGYATD
Sbjct: 111 FTKCPEEIGAGDQGHMFGYATD 132
Score = 89.4 bits (212), Expect = 2e-18
Identities = 42/65 (64%), Positives = 48/65 (73%)
Frame = +3
Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMCFCVAKSHPKLTW 293
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT M + K T
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 294 IIKKL 308
+K+
Sbjct: 64 DYEKI 68
Score = 52.0 bits (119), Expect = 4e-07
Identities = 24/46 (52%), Positives = 31/46 (67%)
Frame = +1
Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGA 642
++PL+ VLA KL ++ E+R+NG W RPD KTQVT EY GA
Sbjct: 137 LMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGA 182
Score = 33.9 bits (74), Expect = 0.11
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +3
Query: 645 VPQRVHTVVVSLQHSEKITLETLRDEIRE 731
VP RVHTV++S QH E +T + + +++E
Sbjct: 184 VPIRVHTVLISTQHDETVTNDEIARDLKE 212
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 92.7 bits (220), Expect = 2e-19
Identities = 42/82 (51%), Positives = 60/82 (73%), Gaps = 2/82 (2%)
Frame = +2
Query: 254 VLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN 433
V++ GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GVH +
Sbjct: 51 VMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGH 110
Query: 434 --RNDEEVGAGDQGLMFGYATD 493
+ E++GAGDQG MFGYATD
Sbjct: 111 LTKKPEDIGAGDQGHMFGYATD 132
Score = 87.0 bits (206), Expect = 1e-17
Identities = 39/47 (82%), Positives = 42/47 (89%)
Frame = +3
Query: 114 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGM 254
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT M
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNM 50
Score = 54.0 bits (124), Expect = 1e-07
Identities = 26/46 (56%), Positives = 31/46 (67%)
Frame = +1
Query: 505 MLPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGA 642
++PLT VLA KL K+ E+R+N W RPD KTQVT EY GGA
Sbjct: 137 LMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGA 182
Score = 33.5 bits (73), Expect = 0.15
Identities = 12/29 (41%), Positives = 21/29 (72%)
Frame = +3
Query: 645 VPQRVHTVVVSLQHSEKITLETLRDEIRE 731
+P RVHTV++S QH E +T + + +++E
Sbjct: 184 IPIRVHTVLISTQHDETVTNDEIAADLKE 212
>At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 334
Score = 27.5 bits (58), Expect = 9.8
Identities = 20/78 (25%), Positives = 36/78 (46%), Gaps = 4/78 (5%)
Frame = +3
Query: 42 PETSKMNGYAKTNGHSYDMEDGSVFLFTSESVGEGHPDKMCD-QISDAILDAHLNQDPD- 215
PE + T+G+ Y +E+G VF S G+G + + D +I D + + +
Sbjct: 244 PEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAEL 303
Query: 216 -AKVACET-ITKTGMCFC 263
A+ C +T G+ +C
Sbjct: 304 LAEKRCVAHLTGEGIAYC 321
>At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 442
Score = 27.5 bits (58), Expect = 9.8
Identities = 20/78 (25%), Positives = 36/78 (46%), Gaps = 4/78 (5%)
Frame = +3
Query: 42 PETSKMNGYAKTNGHSYDMEDGSVFLFTSESVGEGHPDKMCD-QISDAILDAHLNQDPD- 215
PE + T+G+ Y +E+G VF S G+G + + D +I D + + +
Sbjct: 352 PEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAEL 411
Query: 216 -AKVACET-ITKTGMCFC 263
A+ C +T G+ +C
Sbjct: 412 LAEKRCVAHLTGEGIAYC 429
>At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 443
Score = 27.5 bits (58), Expect = 9.8
Identities = 20/78 (25%), Positives = 36/78 (46%), Gaps = 4/78 (5%)
Frame = +3
Query: 42 PETSKMNGYAKTNGHSYDMEDGSVFLFTSESVGEGHPDKMCD-QISDAILDAHLNQDPD- 215
PE + T+G+ Y +E+G VF S G+G + + D +I D + + +
Sbjct: 353 PEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAEL 412
Query: 216 -AKVACET-ITKTGMCFC 263
A+ C +T G+ +C
Sbjct: 413 LAEKRCVAHLTGEGIAYC 430
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,639,675
Number of Sequences: 28952
Number of extensions: 401708
Number of successful extensions: 1119
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1059
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1114
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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