Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm1323
(630 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 52 9e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 47 3e-06
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 42 6e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.55
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 0.97
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 26 5.2
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 5.2
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 25 9.0
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 9.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 51.6 bits (118), Expect = 9e-08
Identities = 25/65 (38%), Positives = 39/65 (60%)
Frame = +1
Query: 265 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 444
E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++ D DG+I Y
Sbjct: 85 EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DTDGDGVINYEE 142
Query: 445 FLKKV 459
F + +
Sbjct: 143 FSRVI 147
Score = 33.1 bits (72), Expect = 0.034
Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 3/84 (3%)
Frame = +2
Query: 17 MSDLSKNDVEMASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI-XXXXXXXXX 187
M+ + D ++A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 188 XXXXXXXXXFLPIYSQAKKDKDRE 259
FL + ++ KD D E
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE 84
Score = 29.1 bits (62), Expect = 0.55
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +1
Query: 268 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 447
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 448 L 450
L
Sbjct: 71 L 71
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 46.8 bits (106), Expect = 3e-06
Identities = 24/71 (33%), Positives = 43/71 (60%)
Frame = +1
Query: 253 PGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMI 432
PG E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+
Sbjct: 73 PGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMV 129
Query: 433 PYAAFLKKVMA 465
Y F++ ++A
Sbjct: 130 NYHDFVQMILA 140
Score = 42.3 bits (95), Expect = 6e-05
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +2
Query: 59 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 160
AFS++D G G+I ++GDLLRA NPTLA I
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI 44
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 42.3 bits (95), Expect = 6e-05
Identities = 20/67 (29%), Positives = 41/67 (61%)
Frame = +1
Query: 265 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 444
E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP + G Y
Sbjct: 78 EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TNSGSFDYYD 135
Query: 445 FLKKVMA 465
F++++MA
Sbjct: 136 FVQRIMA 142
Score = 30.7 bits (66), Expect = 0.18
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +1
Query: 265 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 444
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.1 bits (62), Expect = 0.55
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +1
Query: 265 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 399
+D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 37 QDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 26.6 bits (56), Expect = 3.0
Identities = 17/66 (25%), Positives = 29/66 (43%)
Frame = +1
Query: 265 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 444
E+ +L+D +E G + L L E +DD E+ + ++ D DG I
Sbjct: 110 EEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQDGEINEQE 167
Query: 445 FLKKVM 462
F+ +M
Sbjct: 168 FIAIMM 173
Score = 25.8 bits (54), Expect = 5.2
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +2
Query: 26 LSKNDVEMASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 142
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 0.97
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = -3
Query: 613 APPEELSPPRALPAPVPQSRASVF*GPSHRTFVAGRI 503
APP PP A P P+P S A P R+ + +I
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRSALLQQI 1756
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.8 bits (54), Expect = 5.2
Identities = 20/71 (28%), Positives = 32/71 (45%)
Frame = +3
Query: 57 SPSQSTTLKAKVRSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKSSFPSTAK 236
SP Q T+ + MP +A + ++P + SRN+ + RR+ + S S PS
Sbjct: 536 SPVQPTSYNRTLPPMPEVVAYKGDE--ESP--RVSRNTSLARRKPLPDTESHSPSPSATS 591
Query: 237 QRKTKTGSV*R 269
K S+ R
Sbjct: 592 SIKKNPSSIFR 602
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 5.2
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 352 LGEKLDDSEVAEVTKDCMDPED 417
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 25.0 bits (52), Expect = 9.0
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +3
Query: 42 LKWRLSPSQSTTLKAKVRSMPSTL 113
+ WRL+P+ S ++ K + P+TL
Sbjct: 2229 INWRLAPAYSPSIDEKQSNEPNTL 2252
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 9.0
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +2
Query: 17 MSDLSKNDV-EMASFAFSIYDFEGKGKID 100
+++L DV SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,219,837
Number of Sequences: 5004
Number of extensions: 40789
Number of successful extensions: 139
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -