Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm1271
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g59510.1 68418.m07458 expressed protein 32 0.32
At4g32630.1 68417.m04645 hypothetical protein 30 1.3
At5g59760.1 68418.m07490 hypothetical protein 30 1.7
At5g13520.1 68418.m01561 peptidase M1 family protein similar to ... 29 2.2
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 28 5.2
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 28 5.2
At1g02460.1 68414.m00195 glycoside hydrolase family 28 protein /... 28 5.2
At5g19260.1 68418.m02293 expressed protein various predicted pro... 28 6.8
At3g42100.1 68416.m04322 AT hook motif-containing protein-relate... 28 6.8
At5g45190.1 68418.m05547 cyclin family protein similar to cyclin... 27 9.0
At4g11380.1 68417.m01835 beta-adaptin, putative strong similarit... 27 9.0
>At5g59510.1 68418.m07458 expressed protein
Length = 144
Score = 32.3 bits (70), Expect = 0.32
Identities = 28/80 (35%), Positives = 41/80 (51%), Gaps = 1/80 (1%)
Frame = -3
Query: 464 TQPSTSSIKELFNTSSNLNSTRYHTHKKLFTSLV*KFPTSKSTKWSHSLTISTSMP-STL 288
T+ S+SS K +F S + T Y + + +F PTS + + S + ST P T
Sbjct: 33 TKTSSSSSKPVFTRSFSTKPTSYSSSEPIFRRSFSAKPTSSKSPFL-SRSGSTKCPVDTS 91
Query: 287 STSVKKNSRALSRLQGPSTT 228
STS SR+LS+ +G S T
Sbjct: 92 STSKCSISRSLSQ-KGASVT 110
>At4g32630.1 68417.m04645 hypothetical protein
Length = 531
Score = 30.3 bits (65), Expect = 1.3
Identities = 28/89 (31%), Positives = 41/89 (46%), Gaps = 7/89 (7%)
Frame = -3
Query: 470 PGTQPSTSSIKELFNTSSNLNSTRYHTHKKLFTSL-------V*KFPTSKSTKWSHSLTI 312
P P T++ EL+NTS +++ST +T L T V F TS +T +HS +
Sbjct: 316 PSVVPGTNNY-ELWNTS-DISSTENYTAVNLGTQTMPGIPDSVTSFATSPTTAHAHSGSS 373
Query: 311 STSMPSTLSTSVKKNSRALSRLQGPSTTS 225
+P K + L+ QGPS S
Sbjct: 374 GPVVPVAPDNLNTKETATLANNQGPSDFS 402
>At5g59760.1 68418.m07490 hypothetical protein
Length = 221
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = -3
Query: 347 SKSTKWSHSLTISTSMPSTLSTSVKKNSRALSRLQGPS 234
+KS +SHS +S+S S LST + + + L+ L+ P+
Sbjct: 74 TKSNSFSHSQDVSSSSSSELSTFLNIHPQPLTNLEDPT 111
>At5g13520.1 68418.m01561 peptidase M1 family protein similar to
SP|P09960 Leukotriene A-4 hydrolase (EC 3.3.2.6) (LTA-4
hydrolase) {Homo sapiens}; contains Pfam profile
PF01433: Peptidase family M1
Length = 616
Score = 29.5 bits (63), Expect = 2.2
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +1
Query: 397 YLVLFKFDDVLNNSFIELVEGWVPGRSLVEVKSTRNKLYWLKGLG 531
Y+ FKF + N+F+E ++ +PG ++ N W +G+G
Sbjct: 423 YIATFKFKSIDTNTFLEFLKANIPG-----IEKEINLQLWTEGVG 462
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 18/74 (24%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Frame = +1
Query: 229 VVDGPCNRERALEFFFTEVDSVEGIEVEMVKECDHFVDFDVGNFQTNEVKSFLCVWYL-V 405
VV ++++ + FF + + + +V + DH + V F T V S +CV L
Sbjct: 283 VVSAAVHKQKTMFFFLIQTEYGDVFKVTLDHNGDHVSELKVKYFDTIPVASSICVLKLGF 342
Query: 406 LFKFDDVLNNSFIE 447
LF + N+ +
Sbjct: 343 LFSASEFGNHGLYQ 356
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 28.3 bits (60), Expect = 5.2
Identities = 18/74 (24%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Frame = +1
Query: 229 VVDGPCNRERALEFFFTEVDSVEGIEVEMVKECDHFVDFDVGNFQTNEVKSFLCVWYL-V 405
VV ++++ + FF + + + +V + DH + V F T V S +CV L
Sbjct: 283 VVSAAVHKQKTMFFFLIQTEYGDVFKVTLDHNGDHVSELKVKYFDTIPVASSICVLKLGF 342
Query: 406 LFKFDDVLNNSFIE 447
LF + N+ +
Sbjct: 343 LFSASEFGNHGLYQ 356
>At1g02460.1 68414.m00195 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase PG1 GI:5669846, PG2 GI:5669848 from
(Glycine max); contains PF00295: Glycosyl hydrolases
family 28 (polygalacturonases)
Length = 491
Score = 28.3 bits (60), Expect = 5.2
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1
Query: 229 VVDGPCNRERALEFFFTEVDSVEGIEVE 312
+V GPC+ AL FF + VEG++++
Sbjct: 215 IVTGPCDSPIALRFFMSSNLRVEGLQIK 242
>At5g19260.1 68418.m02293 expressed protein various predicted
proteins
Length = 288
Score = 27.9 bits (59), Expect = 6.8
Identities = 20/78 (25%), Positives = 33/78 (42%)
Frame = -3
Query: 464 TQPSTSSIKELFNTSSNLNSTRYHTHKKLFTSLV*KFPTSKSTKWSHSLTISTSMPSTLS 285
+QP ++K SS+ TR K S P S S+ WS ++S+ S+
Sbjct: 32 SQPFGLALKSHLLDSSHAEDTRNRNDDKAAAS-----PVSDSSGWSSLQSLSSGSSSSTK 86
Query: 284 TSVKKNSRALSRLQGPST 231
T+ + +Q PS+
Sbjct: 87 TTTSSEKESSYYVQRPSS 104
>At3g42100.1 68416.m04322 AT hook motif-containing protein-related
very low similarity to SP|Q9UUA2 DNA repair and
recombination protein pif1, mitochondrial precursor
{Schizosaccharomyces pombe}; weak hit to Pfam profile
PF02178: AT hook motif
Length = 1752
Score = 27.9 bits (59), Expect = 6.8
Identities = 22/74 (29%), Positives = 35/74 (47%)
Frame = -3
Query: 464 TQPSTSSIKELFNTSSNLNSTRYHTHKKLFTSLV*KFPTSKSTKWSHSLTISTSMPSTLS 285
T P TS+ +F +NL Y + T S S K +HS I +++ S L+
Sbjct: 45 TVPLTSAFSRVFKDITNLG---YSSVGSFLTPS--NNQQSYSAKKTHSTNIGSTVVSGLA 99
Query: 284 TSVKKNSRALSRLQ 243
+ V+ NSR + + Q
Sbjct: 100 SMVQANSRHMKQSQ 113
>At5g45190.1 68418.m05547 cyclin family protein similar to cyclin T1
[Equus caballus] GI:5052355; contains Pfam profile
PF00134: Cyclin, N-terminal domain
Length = 579
Score = 27.5 bits (58), Expect = 9.0
Identities = 22/60 (36%), Positives = 29/60 (48%)
Frame = -1
Query: 220 HKPFTVTIDIKSDVATNAVVKMFLGPKYDENGFPFSLEDNWMNFYELDWFVQKVNPGQSQ 41
H+ F K+D T A V MFL K +E P L+D YE+ + K +PG SQ
Sbjct: 89 HRFFFRQSHAKNDRRTIATVCMFLAGKVEET--PRPLKDVIFVSYEI---INKKDPGASQ 143
>At4g11380.1 68417.m01835 beta-adaptin, putative strong similarity
to SP|Q10567 Adapter-related protein complex 1 beta 1
subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
[Drosophila melanogaster] GI:434902; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 894
Score = 27.5 bits (58), Expect = 9.0
Identities = 19/63 (30%), Positives = 29/63 (46%)
Frame = -3
Query: 632 GNYWQTNADLFEEDFLQFYQRSYEVNARRVLGAAPKPFNQYNLFLVLLTSTKLRPGTQPS 453
G Q +A L +D FY +E N++ VL FN+ L S ++ P P+
Sbjct: 676 GQGLQISAQLSRKDGQVFYSMLFENNSQSVLDGFMIQFNKNTFGLAAAGSLQI-PPLHPA 734
Query: 452 TSS 444
TS+
Sbjct: 735 TSA 737
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,102,408
Number of Sequences: 28952
Number of extensions: 245517
Number of successful extensions: 782
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 782
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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