SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVm1268
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ...    29   3.9  
At5g42370.1 68418.m05159 expressed protein                             29   3.9  
At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic...    29   3.9  
At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox...    28   5.2  
At5g24290.2 68418.m02858 integral membrane family protein contai...    28   6.8  
At4g21520.1 68417.m03110 transducin family protein / WD-40 repea...    28   6.8  
At3g20780.1 68416.m02628 topoisomerase 6 subunit B (TOP6B) nearl...    28   6.8  
At3g14010.1 68416.m01769 hydroxyproline-rich glycoprotein family...    28   6.8  
At1g21020.1 68414.m02630 hypothetical protein similar to At3g265...    28   6.8  
At5g22390.1 68418.m02612 expressed protein                             27   9.0  

>At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to
           autophagy 7 [Arabidopsis thaliana] GI:19912147; contains
           Pfam profile PF00899: ThiF family
          Length = 697

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = -2

Query: 234 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 100
           P  ++G    CG  +V NH++LL +S+ L+      ++S  +R +
Sbjct: 42  PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86


>At5g42370.1 68418.m05159 expressed protein
          Length = 447

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -3

Query: 173 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 63
           P Y +  +   Q+ +K +P PL  + R L W TPS  R
Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322


>At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical
           to low affinity calcium antiporter CAX2 (GI:1488267)
           [Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA)
           Family member PMID:11500563
          Length = 441

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
 Frame = +3

Query: 36  SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 167
           S++   SL TS  +  PK P+++   S K  +FC  L++ +P+ P+
Sbjct: 41  SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86


>At1g72520.1 68414.m08386 lipoxygenase, putative similar to
           lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140
           [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis
           thaliana]
          Length = 926

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 14/33 (42%), Positives = 19/33 (57%)
 Frame = -1

Query: 229 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 131
           +S +  P  R    ++PYLPS    G RTLR+K
Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247


>At5g24290.2 68418.m02858 integral membrane family protein contains
           Pfam domain PF01988: Integral membrane protein
          Length = 534

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 17/54 (31%), Positives = 23/54 (42%), Gaps = 1/54 (1%)
 Frame = -1

Query: 517 QFLGRIILKY*LAKTNI-THEHRPDPAPAHPLPVQTRHAPVLRANPYSEVTDPI 359
           +FL  ++  Y   K N+   E R   A   P PV T    +   N  S+  DPI
Sbjct: 26  EFLVDLLESYRFGKDNVPAREFRSKAAATAPAPVNTTEIELEEDNDGSQAQDPI 79


>At4g21520.1 68417.m03110 transducin family protein / WD-40 repeat
           family protein contains 5 WD-40 repeats (PF00400);
           similar to guanine nucleotide-binding protein beta 5
           (GI:1001939) [Mesocricetus auratus]
          Length = 425

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 11/27 (40%), Positives = 19/27 (70%)
 Frame = +3

Query: 210 GGVRILSADLENSGEDVRGDVAPVRTH 290
           GG+++ S + ++S   +R DV+P RTH
Sbjct: 12  GGLKVESGEQKSSWPTMRFDVSPYRTH 38


>At3g20780.1 68416.m02628 topoisomerase 6 subunit B (TOP6B) nearly
           identical to topoisomerase 6 subunit B [Arabidopsis
           thaliana] GI:12331188
          Length = 670

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = -1

Query: 523 YIQFLGRIILKY*LAKTNITHEHRPDPAPAHPLPVQTRHAP 401
           Y QFL R I +       I    R D  P  P+P++T+H P
Sbjct: 284 YAQFLFRFISETPEKNVTIKFTRRTDVMP--PIPIETKHHP 322


>At3g14010.1 68416.m01769 hydroxyproline-rich glycoprotein family
           protein similar to Mrs16p (GI:2737884) [Saccharomyces
           cerevisiae]; weak similarity to ataxin-2 related protein
           (GI:1679686) [Homo sapiens]
          Length = 595

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = -3

Query: 467 NARTSTRPGTGASASRPNPTRPGPQSQS 384
           +++T +    G SASR + +RPGP + S
Sbjct: 419 SSKTKSESSFGQSASRSSESRPGPSTSS 446


>At1g21020.1 68414.m02630 hypothetical protein similar to At3g26530,
           At1g08760, At1g08740, At2g29240, At1g35650
          Length = 751

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 16/34 (47%), Positives = 21/34 (61%)
 Frame = -3

Query: 413 PTRPGPQSQSLFRSYGSNLPTSLTYIILSTRGSS 312
           PTRPG +S SL R  G+N PT+ T +  +   SS
Sbjct: 499 PTRPGLRS-SLKRKRGNNGPTAATILTPAVDNSS 531


>At5g22390.1 68418.m02612 expressed protein
          Length = 202

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = +3

Query: 108 ERSGKSFLFCLSVRVPWNPIEG 173
           + S KSFL  LS   PWNP +G
Sbjct: 17  DNSPKSFLDTLSSSSPWNPSKG 38


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,425,940
Number of Sequences: 28952
Number of extensions: 374635
Number of successful extensions: 1270
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1267
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -