BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm1224 (630 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 138 6e-34 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 138 6e-34 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 138 6e-34 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 54 2e-08 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.079 SPAPB1E7.03 |rpc82||DNA-directed RNA polymerase III complex subu... 26 5.2 SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 6.8 SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 25 9.0 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 138 bits (334), Expect = 6e-34 Identities = 61/82 (74%), Positives = 72/82 (87%) Frame = -2 Query: 509 ILDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFP 330 +LDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ Sbjct: 358 VLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYA 417 Query: 329 PLGRFAVRDMRQTVAVGVIKAV 264 PLGRFAVRDMRQTVAVGVIKAV Sbjct: 418 PLGRFAVRDMRQTVAVGVIKAV 439 Score = 65.3 bits (152), Expect = 7e-12 Identities = 28/38 (73%), Positives = 32/38 (84%) Frame = -1 Query: 630 ELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 517 ++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS G Sbjct: 317 DIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAG 354 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 138 bits (334), Expect = 6e-34 Identities = 61/82 (74%), Positives = 72/82 (87%) Frame = -2 Query: 509 ILDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFP 330 +LDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ Sbjct: 358 VLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYA 417 Query: 329 PLGRFAVRDMRQTVAVGVIKAV 264 PLGRFAVRDMRQTVAVGVIKAV Sbjct: 418 PLGRFAVRDMRQTVAVGVIKAV 439 Score = 65.3 bits (152), Expect = 7e-12 Identities = 28/38 (73%), Positives = 32/38 (84%) Frame = -1 Query: 630 ELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 517 ++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS G Sbjct: 317 DIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAG 354 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 138 bits (334), Expect = 6e-34 Identities = 61/82 (74%), Positives = 72/82 (87%) Frame = -2 Query: 509 ILDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFP 330 +LDCHTAHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ Sbjct: 358 VLDCHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYA 417 Query: 329 PLGRFAVRDMRQTVAVGVIKAV 264 PLGRFAVRDMRQTVAVGVIKAV Sbjct: 418 PLGRFAVRDMRQTVAVGVIKAV 439 Score = 65.3 bits (152), Expect = 7e-12 Identities = 28/38 (73%), Positives = 32/38 (84%) Frame = -1 Query: 630 ELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 517 ++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS G Sbjct: 317 DIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAG 354 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 53.6 bits (123), Expect = 2e-08 Identities = 35/119 (29%), Positives = 59/119 (49%), Gaps = 1/119 (0%) Frame = -2 Query: 614 MLLVTPKTTHLRVLQILQLKSLCLTILVKSQTVHTILDCHTAHIACKFAEIKEKVDRRTG 435 +L T H I Q+ L L ++ + ++ HTA FA++ K+D+ T Sbjct: 546 VLTSTKNPVHATTRFIAQIAILELPSILTTG-YSCVMHIHTAVEEVSFAKLLHKLDK-TN 603 Query: 434 KSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 261 + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++ Sbjct: 604 RKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 31.9 bits (69), Expect = 0.079 Identities = 15/31 (48%), Positives = 20/31 (64%) Frame = -2 Query: 362 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 270 PLC+ +E P LGRF +R TVA G++K Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589 >SPAPB1E7.03 |rpc82||DNA-directed RNA polymerase III complex subunit Rpc82 |Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.8 bits (54), Expect = 5.2 Identities = 13/41 (31%), Positives = 21/41 (51%) Frame = -2 Query: 542 TILVKSQTVHTILDCHTAHIACKFAEIKEKVDRRTGKSTEV 420 +ILV + H ++ + K A + V+RR GKST + Sbjct: 255 SILVPDLSAHVRINSGKLSVLSKNARLTHWVERRIGKSTSL 295 >SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = +1 Query: 385 MAASPDLMDFGLTSVDLPVRRSTFS 459 ++ +PDL D L+SVD P++ +T S Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51 >SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/32 (40%), Positives = 14/32 (43%) Frame = -3 Query: 139 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 44 G S E S+ IFY FYNL KV Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,584,636 Number of Sequences: 5004 Number of extensions: 50508 Number of successful extensions: 142 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 140 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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