BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm1207 (750 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g60230.1 68414.m06783 radical SAM domain-containing protein ... 30 1.9 At5g18190.1 68418.m02135 protein kinase family protein contains ... 29 2.5 At1g76965.1 68414.m08961 glycine-rich protein 29 2.5 At2g02800.2 68415.m00225 protein kinase (APK2b) identical to pro... 29 3.3 At2g02800.1 68415.m00224 protein kinase (APK2b) identical to pro... 29 3.3 At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 4.4 At5g42370.1 68418.m05159 expressed protein 29 4.4 At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 4.4 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 5.8 >At1g60230.1 68414.m06783 radical SAM domain-containing protein contains Pfam profile PF04055: radical SAM domain protein Length = 458 Score = 29.9 bits (64), Expect = 1.9 Identities = 15/35 (42%), Positives = 21/35 (60%) Frame = -3 Query: 118 FPDLSAASSGHFGLPRRTLVFKDEGTIIETVPLPC 14 F D+ +AS G R+ L D+G +IETV +PC Sbjct: 161 FKDIRSASDG----TRKILFTLDDGLVIETVVIPC 191 >At5g18190.1 68418.m02135 protein kinase family protein contains Pfam domains, PF00069: Protein kinase domain Length = 691 Score = 29.5 bits (63), Expect = 2.5 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -3 Query: 283 VRTGATSPRTPSPEFSRSAESIRTPPQ 203 +R+GA R PSP+ + A++I PPQ Sbjct: 4 LRSGARRLRQPSPQVTGQADNIELPPQ 30 >At1g76965.1 68414.m08961 glycine-rich protein Length = 158 Score = 29.5 bits (63), Expect = 2.5 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -1 Query: 444 PGTGRIRFPSKPDTPRSSEPILIPKLRIQFAD 349 PG + FP KP+ P P +P+L + F D Sbjct: 93 PGAAIVVFPKKPEEPVKVVPTPMPQLNLFFGD 124 >At2g02800.2 68415.m00225 protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 Length = 426 Score = 29.1 bits (62), Expect = 3.3 Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 1/60 (1%) Frame = -1 Query: 510 LRPHYIKILTR*NEHNARTSTRPGTGRIRFPSKPDTPRSSEPILIPKLRIQFA-DFPYLH 334 LRP ++L + ++ ST+PGTG ++ D+PR S ++ K +++ D P LH Sbjct: 352 LRPKMSEVLAKLDQLE---STKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSYDRPLLH 408 >At2g02800.1 68415.m00224 protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 Length = 426 Score = 29.1 bits (62), Expect = 3.3 Identities = 19/60 (31%), Positives = 33/60 (55%), Gaps = 1/60 (1%) Frame = -1 Query: 510 LRPHYIKILTR*NEHNARTSTRPGTGRIRFPSKPDTPRSSEPILIPKLRIQFA-DFPYLH 334 LRP ++L + ++ ST+PGTG ++ D+PR S ++ K +++ D P LH Sbjct: 352 LRPKMSEVLAKLDQLE---STKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSYDRPLLH 408 >At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to autophagy 7 [Arabidopsis thaliana] GI:19912147; contains Pfam profile PF00899: ThiF family Length = 697 Score = 28.7 bits (61), Expect = 4.4 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -1 Query: 231 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 97 P ++G CG +V NH++LL +S+ L+ ++S +R + Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86 >At5g42370.1 68418.m05159 expressed protein Length = 447 Score = 28.7 bits (61), Expect = 4.4 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 170 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 60 P Y + + Q+ +K +P PL + R L W TPS R Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322 >At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical to low affinity calcium antiporter CAX2 (GI:1488267) [Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 441 Score = 28.7 bits (61), Expect = 4.4 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%) Frame = +3 Query: 33 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 164 S++ SL TS + PK P+++ S K +FC L++ +P+ P+ Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 28.3 bits (60), Expect = 5.8 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -3 Query: 226 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 128 +S + P R ++PYLPS G RTLR+K Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,336,956 Number of Sequences: 28952 Number of extensions: 383278 Number of successful extensions: 1173 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1172 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1663169840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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