Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm1100
(770 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 154 1e-38
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 154 1e-38
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 154 1e-38
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 49 8e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 34 0.026
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.10
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 0.97
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 28 1.7
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 27 2.2
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.9
SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 27 3.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 154 bits (374), Expect = 1e-38
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -2
Query: 760 TEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 581
TEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+
Sbjct: 285 TEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVII 344
Query: 580 LNHPGQISNGYTPVLDCHTAHIAC 509
LNHPGQIS GY+PVLDCHTAHIAC
Sbjct: 345 LNHPGQISAGYSPVLDCHTAHIAC 368
Score = 117 bits (281), Expect = 2e-27
Identities = 56/89 (62%), Positives = 67/89 (75%), Gaps = 1/89 (1%)
Frame = -1
Query: 527 HCPH-CLQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 351
H H +FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 350 FAVRDMRQTVAVGVIKAVNFKEAGGGKVT 264
FAVRDMRQTVAVGVIKAV G KVT
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 154 bits (374), Expect = 1e-38
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -2
Query: 760 TEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 581
TEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+
Sbjct: 285 TEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVII 344
Query: 580 LNHPGQISNGYTPVLDCHTAHIAC 509
LNHPGQIS GY+PVLDCHTAHIAC
Sbjct: 345 LNHPGQISAGYSPVLDCHTAHIAC 368
Score = 117 bits (281), Expect = 2e-27
Identities = 56/89 (62%), Positives = 67/89 (75%), Gaps = 1/89 (1%)
Frame = -1
Query: 527 HCPH-CLQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 351
H H +FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 350 FAVRDMRQTVAVGVIKAVNFKEAGGGKVT 264
FAVRDMRQTVAVGVIKAV G KVT
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 154 bits (374), Expect = 1e-38
Identities = 68/84 (80%), Positives = 75/84 (89%)
Frame = -2
Query: 760 TEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIV 581
TEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+
Sbjct: 285 TEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVII 344
Query: 580 LNHPGQISNGYTPVLDCHTAHIAC 509
LNHPGQIS GY+PVLDCHTAHIAC
Sbjct: 345 LNHPGQISAGYSPVLDCHTAHIAC 368
Score = 117 bits (281), Expect = 2e-27
Identities = 56/89 (62%), Positives = 67/89 (75%), Gaps = 1/89 (1%)
Frame = -1
Query: 527 HCPH-CLQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGR 351
H H +FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421
Query: 350 FAVRDMRQTVAVGVIKAVNFKEAGGGKVT 264
FAVRDMRQTVAVGVIKAV G KVT
Sbjct: 422 FAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 48.8 bits (111), Expect = 8e-07
Identities = 25/74 (33%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Frame = -1
Query: 512 LQFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 333
+ FA++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD
Sbjct: 590 VSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQ 648
Query: 332 RQTVAVG-VIKAVN 294
TVAVG V+K ++
Sbjct: 649 GTTVAVGKVVKILD 662
Score = 41.1 bits (92), Expect = 2e-04
Identities = 22/70 (31%), Positives = 36/70 (51%)
Frame = -2
Query: 730 EALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNG 551
E + ++ GD V V+ +++ GYV +KN P F AQ+ +L P ++ G
Sbjct: 519 EEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTG 576
Query: 550 YTPVLDCHTA 521
Y+ V+ HTA
Sbjct: 577 YSCVMHIHTA 586
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 33.9 bits (74), Expect = 0.026
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = -2
Query: 760 TEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 644
T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 296 TTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.10
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -1
Query: 395 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 303
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 0.97
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +1
Query: 418 MAASPDLMDFGLTSVDLPVRRSTFSLISANCRQCGQCGNPRLVCNRLRFDQDG 576
++ +PDL D L+SVD P++ +T C + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNT----------CSESGNQYLLCD---FNRDG 66
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 27.9 bits (59), Expect = 1.7
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +3
Query: 408 QVDNGCISRLDGFWVDFSRFTSTTVNFFFDFCKLQAMWAVWQSKTGV 548
+V C + ++FS T+ T N F A ++VW+ ++G+
Sbjct: 198 EVTENCAAAASSLVLEFSMLTALTGNNKFKASAENAFFSVWKRRSGI 244
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 27.5 bits (58), Expect = 2.2
Identities = 11/43 (25%), Positives = 20/43 (46%)
Frame = +1
Query: 430 PDLMDFGLTSVDLPVRRSTFSLISANCRQCGQCGNPRLVCNRL 558
P ++DF + ++ V+ + + CG PR VC+ L
Sbjct: 279 PSMIDFRIGFIEKAVKHPNADSLYVSTIHCGDAEGPRTVCSGL 321
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 659 QFLDGHVLYVETYIVSRYSFLESFV 733
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC23H4.09 |cdb4||curved DNA-binding protein
Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 381
Score = 26.6 bits (56), Expect = 3.9
Identities = 14/48 (29%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
Frame = +3
Query: 633 ESPAT*PRRNSLTDTFFTLKP---TLSPGTASWRASWCISTDLTSVGC 767
E P T P + + LK T+ PG +W+ + + TS GC
Sbjct: 134 EEPVTGPAADVIAAASAALKAAQRTIKPGNTNWQVTDIVDKIATSYGC 181
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,225,883
Number of Sequences: 5004
Number of extensions: 65786
Number of successful extensions: 180
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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