BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm1076 (781 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 35 0.011 SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 33 0.061 SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosac... 32 0.11 SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces po... 31 0.19 SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomy... 31 0.19 SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch... 30 0.43 SPBC1539.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 28 1.7 SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 27 2.3 SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 27 3.0 SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 27 3.0 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 4.0 SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex sub... 26 5.3 SPBC16A3.18 |cip1||RNA-binding protein Cip1|Schizosaccharomyces ... 26 7.0 SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 26 7.0 SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 25 9.2 SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 25 9.2 >SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 35.1 bits (77), Expect = 0.011 Identities = 18/79 (22%), Positives = 37/79 (46%), Gaps = 1/79 (1%) Frame = +3 Query: 6 WVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQVKSGTIFDDFLITDDPAAAKERG-EVIK 182 W +I NP+Y D + + VG +LW ++ F + + A+ G E Sbjct: 363 WYPRKIPNPDYFDDDHPSHFGPLYGVGFELWTMQPNIRFSNIYVGHSIEDAERLGNETFL 422 Query: 183 KRQEGEKKMKSEQDEAERE 239 + + E+++ S+Q+ E++ Sbjct: 423 PKLKAERELLSKQESMEKQ 441 >SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 32.7 bits (71), Expect = 0.061 Identities = 14/32 (43%), Positives = 23/32 (71%) Frame = +3 Query: 159 KERGEVIKKRQEGEKKMKSEQDEAEREKEKAE 254 +++ E +K++E EKK+K +Q EA+REK E Sbjct: 659 RQKREEKQKQKEREKKLKKQQQEADREKMARE 690 >SPCC1620.10 |cwf26||complexed with Cdc5 protein Cwf26 |Schizosaccharomyces pombe|chr 3|||Manual Length = 305 Score = 31.9 bits (69), Expect = 0.11 Identities = 14/53 (26%), Positives = 29/53 (54%) Frame = +3 Query: 153 AAKERGEVIKKRQEGEKKMKSEQDEAEREKEKAESLXXXXXXXXXXXXLETQR 311 A R +++ R+E ++K+K +++EA R+KE+ + + LE Q+ Sbjct: 160 ATGRRIDLVLARKEAKRKLKEKEEEARRQKEQQQGVVQVRQQKEYLKELERQK 212 >SPAC29A4.04c |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 31.1 bits (67), Expect = 0.19 Identities = 15/38 (39%), Positives = 24/38 (63%) Frame = +3 Query: 141 DDPAAAKERGEVIKKRQEGEKKMKSEQDEAEREKEKAE 254 D+ AAK+ + KK + EKK K E+ E + +K+K+E Sbjct: 437 DEDEAAKKEEKRRKKEAKKEKKEKKEKKEKKEKKKKSE 474 >SPBC25B2.05 |mis3||rRNA processing protein Mis3|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 31.1 bits (67), Expect = 0.19 Identities = 12/29 (41%), Positives = 21/29 (72%) Frame = +3 Query: 162 ERGEVIKKRQEGEKKMKSEQDEAEREKEK 248 E GE K++E E+K ++E+ E ++EK+K Sbjct: 276 ESGEYFLKKEEKERKKRAEKKEQQKEKKK 304 >SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 681 Score = 29.9 bits (64), Expect = 0.43 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -1 Query: 406 LHARTLRTPRYLMSWHSLINHNSLQLI 326 LH +L T RY MSW + H +L+ I Sbjct: 161 LHVGSLLTTRYAMSWEQSVYHEALEWI 187 >SPBC1539.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 386 Score = 27.9 bits (59), Expect = 1.7 Identities = 10/26 (38%), Positives = 18/26 (69%) Frame = +3 Query: 162 ERGEVIKKRQEGEKKMKSEQDEAERE 239 E GE++K+ + EKK+K++ + E E Sbjct: 209 ENGEILKRLVKKEKKLKNDNERLENE 234 >SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 266 Score = 27.5 bits (58), Expect = 2.3 Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = +3 Query: 105 KSGTIF-DDFLITDDPAAAKERGEVIKKRQEGEKKMKSEQDEAEREK 242 KS T+F D+ IT PA+ K+R KK+ G + ++ E ++ Sbjct: 20 KSETVFLDEAAITKPPASKKKRKNRKKKKNNGPSEQFVGNNDLEEQR 66 >SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizosaccharomyces pombe|chr 1|||Manual Length = 1604 Score = 27.1 bits (57), Expect = 3.0 Identities = 12/35 (34%), Positives = 24/35 (68%) Frame = +3 Query: 153 AAKERGEVIKKRQEGEKKMKSEQDEAEREKEKAES 257 AAK R + KK++E E++++ Q+ A+++ E E+ Sbjct: 399 AAKIRADEEKKKKEREEQVRLLQESADKDAEMNEA 433 Score = 26.6 bits (56), Expect = 4.0 Identities = 21/86 (24%), Positives = 39/86 (45%), Gaps = 2/86 (2%) Frame = +3 Query: 3 PWVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQVKSGTIFDDFLITDDPAAAKERGEVIK 182 P V+ I +Y +N K ++C WQ ++ D + T A +E K Sbjct: 636 PRVYKIIQQNQYNRSTNARKTSQLCGREARRWQFRTIKNNKD-MQTKAKRAMRETMVFWK 694 Query: 183 K--RQEGEKKMKSEQDEAEREKEKAE 254 + R E + + K+E++ +R K++ E Sbjct: 695 RNERVERDLRKKAEREALDRAKKEEE 720 >SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 27.1 bits (57), Expect = 3.0 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 3/75 (4%) Frame = +3 Query: 33 EYTPDSNLYKRDEICAVGLDLWQVKSGTIFDDF---LITDDPAAAKERGEVIKKRQEGEK 203 E T N+ ++ E+ +G + +S +F D +I D A +E +RQ G+ Sbjct: 189 EITIVPNVQQQPEL-PLGKNSGNRQSSKLFADLSKPIIETDAAEIEEVSAEKARRQFGKN 247 Query: 204 KMKSEQDEAEREKEK 248 ++ QD E EK+K Sbjct: 248 GIRLSQDAQEEEKQK 262 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 26.6 bits (56), Expect = 4.0 Identities = 15/30 (50%), Positives = 20/30 (66%), Gaps = 3/30 (10%) Frame = +3 Query: 180 KKRQEGEKKMKSEQDEAER---EKEKAESL 260 KK QE EK+ + + +AER EKE+ ESL Sbjct: 42 KKEQEAEKRKAALERKAERERLEKEEMESL 71 >SPBC29A10.03c |rlf2|SPBC365.19c|chromatin remodeling complex subunit Rlf2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 544 Score = 26.2 bits (55), Expect = 5.3 Identities = 10/30 (33%), Positives = 21/30 (70%) Frame = +3 Query: 159 KERGEVIKKRQEGEKKMKSEQDEAEREKEK 248 K+R + + RQ+ +++K E++ +RE+EK Sbjct: 82 KQRAQEERIRQKEAERLKREKERQQREQEK 111 Score = 25.8 bits (54), Expect = 7.0 Identities = 12/33 (36%), Positives = 22/33 (66%) Frame = +3 Query: 162 ERGEVIKKRQEGEKKMKSEQDEAEREKEKAESL 260 E+ E + R + +++ K E+D+ REKE+A+ L Sbjct: 128 EKLEKERIRLQEQQRRKEERDQKLREKEEAQRL 160 >SPBC16A3.18 |cip1||RNA-binding protein Cip1|Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 25.8 bits (54), Expect = 7.0 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +3 Query: 147 PAAAKERGEVIKKRQEGEKKMKSE 218 PAA +E+ E KKRQ E++ K + Sbjct: 282 PAAEREKVEKAKKRQAEERRRKQQ 305 >SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 25.8 bits (54), Expect = 7.0 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +3 Query: 366 DIKYLGVRSVRAWRILFLKPLVAQSSTIP 452 D+K GV RA R+L L +V +T+P Sbjct: 347 DVKSKGVDGARAERVLELINIVTNKNTVP 375 >SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 674 Score = 25.4 bits (53), Expect = 9.2 Identities = 14/41 (34%), Positives = 26/41 (63%), Gaps = 7/41 (17%) Frame = +3 Query: 156 AKERGEVIKKRQEGE-------KKMKSEQDEAEREKEKAES 257 A E +V+K+RQE E K++K ++DE +++++ES Sbjct: 604 AIENIQVVKRRQEKEKSFRQKAKQLKQQEDEDNLKRKRSES 644 >SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizosaccharomyces pombe|chr 1|||Manual Length = 1403 Score = 25.4 bits (53), Expect = 9.2 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = +3 Query: 129 FLITDDPAAAKERGEVIKKRQEGEKKMKSEQDEAEREKEKAES 257 F++ D + K V + ++G K + +EAER+K AES Sbjct: 1233 FMLMDVMDSRKNGWAVKNEVEKGPKTIAEIHEEAERKKALAES 1275 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,754,714 Number of Sequences: 5004 Number of extensions: 54534 Number of successful extensions: 221 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 198 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 219 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 377352472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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