BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm1013 (326 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value DQ325113-1|ABD14127.1| 185|Apis mellifera complementary sex det... 23 1.2 DQ325112-1|ABD14126.1| 185|Apis mellifera complementary sex det... 23 1.2 DQ325111-1|ABD14125.1| 185|Apis mellifera complementary sex det... 23 1.2 DQ325110-1|ABD14124.1| 185|Apis mellifera complementary sex det... 23 1.2 AY569698-1|AAS86651.1| 407|Apis mellifera complementary sex det... 23 1.2 DQ325130-1|ABD14144.1| 174|Apis mellifera complementary sex det... 22 1.6 DQ325129-1|ABD14143.1| 174|Apis mellifera complementary sex det... 22 1.6 DQ325128-1|ABD14142.1| 174|Apis mellifera complementary sex det... 22 1.6 DQ325127-1|ABD14141.1| 174|Apis mellifera complementary sex det... 22 1.6 DQ325125-1|ABD14139.1| 174|Apis mellifera complementary sex det... 22 1.6 DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex det... 22 1.6 AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein. 22 1.6 DQ325101-1|ABD14115.1| 182|Apis mellifera complementary sex det... 22 2.2 DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. 22 2.2 DQ325126-1|ABD14140.1| 174|Apis mellifera complementary sex det... 21 2.9 AB022907-1|BAA86908.1| 615|Apis mellifera glucose oxidase protein. 21 2.9 EF117814-1|ABO38437.1| 570|Apis mellifera cryptochrome 2 protein. 21 3.8 DQ325107-1|ABD14121.1| 176|Apis mellifera complementary sex det... 21 3.8 DQ325087-1|ABD14101.1| 179|Apis mellifera complementary sex det... 21 3.8 DQ325086-1|ABD14100.1| 179|Apis mellifera complementary sex det... 21 3.8 DQ325085-1|ABD14099.1| 179|Apis mellifera complementary sex det... 21 3.8 DQ325084-1|ABD14098.1| 179|Apis mellifera complementary sex det... 21 3.8 AY569710-1|AAS86663.1| 408|Apis mellifera complementary sex det... 21 3.8 AY569709-1|AAS86662.1| 408|Apis mellifera complementary sex det... 21 3.8 AY569708-1|AAS86661.1| 408|Apis mellifera complementary sex det... 21 3.8 AY569706-1|AAS86659.1| 397|Apis mellifera complementary sex det... 21 3.8 AF084556-1|AAC71015.1| 652|Apis mellifera pipsqueak protein. 21 3.8 DQ435336-1|ABD92651.1| 135|Apis mellifera OBP19 protein. 21 5.0 DQ325100-1|ABD14114.1| 183|Apis mellifera complementary sex det... 21 5.0 DQ325099-1|ABD14113.1| 183|Apis mellifera complementary sex det... 21 5.0 DQ325098-1|ABD14112.1| 183|Apis mellifera complementary sex det... 21 5.0 DQ325097-1|ABD14111.1| 183|Apis mellifera complementary sex det... 21 5.0 DQ325096-1|ABD14110.1| 183|Apis mellifera complementary sex det... 21 5.0 DQ325095-1|ABD14109.1| 183|Apis mellifera complementary sex det... 21 5.0 DQ325081-1|ABD14095.1| 186|Apis mellifera complementary sex det... 21 5.0 AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein. 21 5.0 AY569707-1|AAS86660.1| 408|Apis mellifera complementary sex det... 20 6.6 AM076717-1|CAJ28210.1| 501|Apis mellifera serotonin receptor pr... 20 6.6 L10710-1|AAA27730.1| 382|Apis mellifera hyaluronidase protein. 20 8.8 AY569716-1|AAS86669.1| 406|Apis mellifera complementary sex det... 20 8.8 >DQ325113-1|ABD14127.1| 185|Apis mellifera complementary sex determiner protein. Length = 185 Score = 22.6 bits (46), Expect = 1.2 Identities = 12/38 (31%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P PLG + I + R H+ PS F R Sbjct: 126 PVPIYCGNFPPRPLGPWISIQEQIPRFRHIGPSTPFPR 163 >DQ325112-1|ABD14126.1| 185|Apis mellifera complementary sex determiner protein. Length = 185 Score = 22.6 bits (46), Expect = 1.2 Identities = 12/38 (31%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P PLG + I + R H+ PS F R Sbjct: 126 PVPIYCGNFPPRPLGPWISIQEQIPRFRHIGPSTPFPR 163 >DQ325111-1|ABD14125.1| 185|Apis mellifera complementary sex determiner protein. Length = 185 Score = 22.6 bits (46), Expect = 1.2 Identities = 12/38 (31%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P PLG + I + R H+ PS F R Sbjct: 126 PVPIYCGNFPPRPLGPWISIQEQIPRFRHIGPSTPFPR 163 >DQ325110-1|ABD14124.1| 185|Apis mellifera complementary sex determiner protein. Length = 185 Score = 22.6 bits (46), Expect = 1.2 Identities = 12/38 (31%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P PLG + I + R H+ PS F R Sbjct: 126 PVPIYCGNFPPRPLGPWISIQEQIPRFRHIGPSTPFPR 163 >AY569698-1|AAS86651.1| 407|Apis mellifera complementary sex determiner protein. Length = 407 Score = 22.6 bits (46), Expect = 1.2 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 349 PVPVYCGNFPPRPMGPWISIQEQIPRFRHIGPSTSFPR 386 >DQ325130-1|ABD14144.1| 174|Apis mellifera complementary sex determiner protein. Length = 174 Score = 22.2 bits (45), Expect = 1.6 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 115 PVPVYYGNFPPRPMGSWISIQEQIPRFRHIGPSTPFPR 152 >DQ325129-1|ABD14143.1| 174|Apis mellifera complementary sex determiner protein. Length = 174 Score = 22.2 bits (45), Expect = 1.6 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 115 PVPVYYGNFPPRPMGSWISIQEQIPRFRHIGPSTPFPR 152 >DQ325128-1|ABD14142.1| 174|Apis mellifera complementary sex determiner protein. Length = 174 Score = 22.2 bits (45), Expect = 1.6 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 115 PVPVYYGNFPPRPMGSWISIQEQIPRFRHIGPSTPFPR 152 >DQ325127-1|ABD14141.1| 174|Apis mellifera complementary sex determiner protein. Length = 174 Score = 22.2 bits (45), Expect = 1.6 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 115 PVPVYYGNFPPRPMGSWISIQEQIPRFRHIGPSTPFPR 152 >DQ325125-1|ABD14139.1| 174|Apis mellifera complementary sex determiner protein. Length = 174 Score = 22.2 bits (45), Expect = 1.6 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 115 PVPVYYGNFPPRPMGSWISIQEQIPRFRHIGPSTPFPR 152 >DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex determiner protein. Length = 182 Score = 22.2 bits (45), Expect = 1.6 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 123 PVPVYCGNFPPRPMGPWISIQEQIPRFRHIGPSTPFPR 160 >AB207270-1|BAE72137.1| 429|Apis mellifera broad-complex protein. Length = 429 Score = 22.2 bits (45), Expect = 1.6 Identities = 9/21 (42%), Positives = 11/21 (52%) Frame = -2 Query: 88 PDLSAASSGHFGLPRRTLVFK 26 PDL+ S G GLP L + Sbjct: 336 PDLAGTSQGSAGLPSAILAMR 356 >DQ325101-1|ABD14115.1| 182|Apis mellifera complementary sex determiner protein. Length = 182 Score = 21.8 bits (44), Expect = 2.2 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 124 PVPIYCGNFPPRPMGPWISIQEQIPRFRHIGPSTPFPR 161 >DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. Length = 630 Score = 21.8 bits (44), Expect = 2.2 Identities = 9/28 (32%), Positives = 13/28 (46%) Frame = -2 Query: 157 SEPYLPSIGFHGTRTLRQKRKLFPDLSA 74 SE P+ HG R RQ+ + D + Sbjct: 472 SENNYPTTSIHGDRLQRQREEALADFKS 499 >DQ325126-1|ABD14140.1| 174|Apis mellifera complementary sex determiner protein. Length = 174 Score = 21.4 bits (43), Expect = 2.9 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 115 PVPVYYGNFPPRPMGSWISIQEQIPRFRHIGPSTLFPR 152 >AB022907-1|BAA86908.1| 615|Apis mellifera glucose oxidase protein. Length = 615 Score = 21.4 bits (43), Expect = 2.9 Identities = 9/18 (50%), Positives = 11/18 (61%) Frame = -1 Query: 302 LSTGGSSPWRPAADMGRT 249 LSTGGS W ++G T Sbjct: 136 LSTGGSCYWPRGKNLGGT 153 >EF117814-1|ABO38437.1| 570|Apis mellifera cryptochrome 2 protein. Length = 570 Score = 21.0 bits (42), Expect = 3.8 Identities = 8/20 (40%), Positives = 11/20 (55%) Frame = -1 Query: 134 RIPWNSNAQAEKKTLPGPLG 75 +IPW+ N +A K G G Sbjct: 334 QIPWDKNVEALAKWANGQTG 353 >DQ325107-1|ABD14121.1| 176|Apis mellifera complementary sex determiner protein. Length = 176 Score = 21.0 bits (42), Expect = 3.8 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 118 PVPVYYGNFLPRPMGPWISIQEQIPRFRHIGPSTPFPR 155 >DQ325087-1|ABD14101.1| 179|Apis mellifera complementary sex determiner protein. Length = 179 Score = 21.0 bits (42), Expect = 3.8 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 121 PVPVYYGNFLPRPMGPWISIQEQIPRFRHIGPSTPFPR 158 >DQ325086-1|ABD14100.1| 179|Apis mellifera complementary sex determiner protein. Length = 179 Score = 21.0 bits (42), Expect = 3.8 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 121 PVPVYYGNFLPRPMGPWISIQEQIPRFRHIGPSTPFPR 158 >DQ325085-1|ABD14099.1| 179|Apis mellifera complementary sex determiner protein. Length = 179 Score = 21.0 bits (42), Expect = 3.8 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 121 PVPVYYGNFLPRPMGPWISIQEQIPRFRHIGPSTPFPR 158 >DQ325084-1|ABD14098.1| 179|Apis mellifera complementary sex determiner protein. Length = 179 Score = 21.0 bits (42), Expect = 3.8 Identities = 11/38 (28%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ PS F R Sbjct: 121 PVPVYYGNFLPRPMGPWISIQEQIPRFRHIGPSTPFPR 158 >AY569710-1|AAS86663.1| 408|Apis mellifera complementary sex determiner protein. Length = 408 Score = 21.0 bits (42), Expect = 3.8 Identities = 10/38 (26%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 PFP P+G + I + R ++ P F R Sbjct: 349 PFPVYYGNFPPRPMGPFVSIQEQIPRFRYIGPPTSFPR 386 >AY569709-1|AAS86662.1| 408|Apis mellifera complementary sex determiner protein. Length = 408 Score = 21.0 bits (42), Expect = 3.8 Identities = 10/38 (26%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 PFP P+G + I + R ++ P F R Sbjct: 349 PFPVYYGNFPPRPMGPFVSIQEQIPRFRYIGPPTSFPR 386 >AY569708-1|AAS86661.1| 408|Apis mellifera complementary sex determiner protein. Length = 408 Score = 21.0 bits (42), Expect = 3.8 Identities = 10/38 (26%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 PFP P+G + I + R ++ P F R Sbjct: 349 PFPVYYGNFPPRPMGPFVSIQEQIPRFRYIGPPTSFPR 386 >AY569706-1|AAS86659.1| 397|Apis mellifera complementary sex determiner protein. Length = 397 Score = 21.0 bits (42), Expect = 3.8 Identities = 10/38 (26%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 PFP P+G + I + R ++ P F R Sbjct: 338 PFPVYYGNFPPRPMGPFVSIQEQIPRFRYIGPPTSFPR 375 >AF084556-1|AAC71015.1| 652|Apis mellifera pipsqueak protein. Length = 652 Score = 21.0 bits (42), Expect = 3.8 Identities = 8/16 (50%), Positives = 9/16 (56%) Frame = +1 Query: 43 DGVTQSGLKTPPRGPG 90 DG L +PPR PG Sbjct: 128 DGPPSVSLSSPPREPG 143 >DQ435336-1|ABD92651.1| 135|Apis mellifera OBP19 protein. Length = 135 Score = 20.6 bits (41), Expect = 5.0 Identities = 8/17 (47%), Positives = 13/17 (76%) Frame = +1 Query: 184 VSGYSLRTLKIQVRDVR 234 V+ ++ LKIQ+RDV+ Sbjct: 14 VNAMTIEELKIQLRDVQ 30 >DQ325100-1|ABD14114.1| 183|Apis mellifera complementary sex determiner protein. Length = 183 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 124 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 161 >DQ325099-1|ABD14113.1| 183|Apis mellifera complementary sex determiner protein. Length = 183 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 124 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 161 >DQ325098-1|ABD14112.1| 183|Apis mellifera complementary sex determiner protein. Length = 183 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 124 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 161 >DQ325097-1|ABD14111.1| 183|Apis mellifera complementary sex determiner protein. Length = 183 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 124 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 161 >DQ325096-1|ABD14110.1| 183|Apis mellifera complementary sex determiner protein. Length = 183 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 124 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 161 >DQ325095-1|ABD14109.1| 183|Apis mellifera complementary sex determiner protein. Length = 183 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 124 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 161 >DQ325081-1|ABD14095.1| 186|Apis mellifera complementary sex determiner protein. Length = 186 Score = 20.6 bits (41), Expect = 5.0 Identities = 10/38 (26%), Positives = 16/38 (42%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P + P+G + + + R H+ PS F R Sbjct: 127 PVPIYCGNFPSRPMGPWVPMQEQIPRFRHIGPSTPFPR 164 >AB167961-1|BAD51404.1| 554|Apis mellifera E74 protein. Length = 554 Score = 20.6 bits (41), Expect = 5.0 Identities = 7/9 (77%), Positives = 8/9 (88%) Frame = +1 Query: 70 TPPRGPGRV 96 +PPRGPG V Sbjct: 397 SPPRGPGGV 405 >AY569707-1|AAS86660.1| 408|Apis mellifera complementary sex determiner protein. Length = 408 Score = 20.2 bits (40), Expect = 6.6 Identities = 10/38 (26%), Positives = 15/38 (39%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 PFP P+G + I + R ++ P F R Sbjct: 349 PFPVYYGNFPPRPMGPFVSIQEQIPRLRYIGPPTSFPR 386 >AM076717-1|CAJ28210.1| 501|Apis mellifera serotonin receptor protein. Length = 501 Score = 20.2 bits (40), Expect = 6.6 Identities = 9/33 (27%), Positives = 14/33 (42%) Frame = -2 Query: 223 SPEFSRSAESIRTPPQMRCSSRSEPYLPSIGFH 125 SP S + +R CS + P+ + FH Sbjct: 331 SPMTSTKSTIVRNHLNSTCSVTNSPHQKKLRFH 363 >L10710-1|AAA27730.1| 382|Apis mellifera hyaluronidase protein. Length = 382 Score = 19.8 bits (39), Expect = 8.8 Identities = 7/18 (38%), Positives = 10/18 (55%) Frame = +3 Query: 39 VRRGNPKWPEDAAERSGK 92 VRR +P W + E+ K Sbjct: 169 VRREHPFWDDQRVEQEAK 186 >AY569716-1|AAS86669.1| 406|Apis mellifera complementary sex determiner protein. Length = 406 Score = 19.8 bits (39), Expect = 8.8 Identities = 10/38 (26%), Positives = 14/38 (36%) Frame = -2 Query: 319 PFPTLSYRLEALPLGDLLRIWVEPARHLHVHPSPEFSR 206 P P P+G + I + R H+ P F R Sbjct: 347 PVPIYCGNFPPRPMGPWISIQEQIPRFRHIGPPTPFPR 384 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 102,662 Number of Sequences: 438 Number of extensions: 2268 Number of successful extensions: 40 Number of sequences better than 10.0: 40 Number of HSP's better than 10.0 without gapping: 40 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 40 length of database: 146,343 effective HSP length: 50 effective length of database: 124,443 effective search space used: 7217694 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 39 (20.8 bits)
- SilkBase 1999-2023 -