Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0999
(341 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07220.1 68414.m00768 expressed protein 33 0.066
At5g59510.1 68418.m07458 expressed protein 31 0.15
At4g23690.1 68417.m03410 disease resistance-responsive family pr... 30 0.46
At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical... 28 1.4
At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 27 2.5
At5g51590.1 68418.m06396 DNA-binding protein-related contains Pf... 27 3.3
At2g29690.1 68415.m03609 anthranilate synthase, alpha subunit, c... 27 4.3
At1g50410.1 68414.m05650 SNF2 domain-containing protein / helica... 27 4.3
At4g00270.1 68417.m00034 DNA-binding storekeeper protein-related... 26 5.7
At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 26 5.7
At1g78310.1 68414.m09126 VQ motif-containing protein contains PF... 26 5.7
At4g21800.2 68417.m03154 ATP-binding family protein contains Pfa... 26 7.6
At4g21800.1 68417.m03153 ATP-binding family protein contains Pfa... 26 7.6
At2g24980.1 68415.m02987 proline-rich extensin-like family prote... 26 7.6
At2g16405.1 68415.m01878 transducin family protein / WD-40 repea... 26 7.6
At5g06780.1 68418.m00766 emsy N terminus domain-containing prote... 25 10.0
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 25 10.0
At2g04220.1 68415.m00407 hypothetical protein 25 10.0
At1g03475.1 68414.m00329 coproporphyrinogen III oxidase, putativ... 25 10.0
>At1g07220.1 68414.m00768 expressed protein
Length = 507
Score = 32.7 bits (71), Expect = 0.066
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = -1
Query: 248 LRIQFADFPSL-HYSID*RLFTLETCCGYGYEPARHLHVHPSPEFSRSAESIRTPPLLRC 72
L + F F +L Y +D + +T G+ EP H+ P FS + + + +L+C
Sbjct: 26 LALSFFSFTALLFYKVDDFIAQTKTLAGHNLEPTPW-HIFPRKSFSAATKHSQAYRILQC 84
Query: 71 SSRSEPY 51
S S PY
Sbjct: 85 SYFSCPY 91
>At5g59510.1 68418.m07458 expressed protein
Length = 144
Score = 31.5 bits (68), Expect = 0.15
Identities = 25/65 (38%), Positives = 34/65 (52%)
Frame = -2
Query: 334 TSSRPGTGRIRFPSKPDTPRSSEPILIRSYGSNLPTSLPYIILSTRGSSPWRPAADMGTN 155
+SS+P R F +KP + SSEPI RS+ + PTS LS GS+ P T+
Sbjct: 38 SSSKPVFTR-SFSTKPTSYSSSEPIFRRSFSAK-PTSSKSPFLSRSGSTKC-PVDTSSTS 94
Query: 154 RRDIS 140
+ IS
Sbjct: 95 KCSIS 99
>At4g23690.1 68417.m03410 disease resistance-responsive family
protein / dirigent family protein similar to disease
resistance response protein 206-d [Pisum sativum]
gi|508844|gb|AAB18669; similar to dirigent protein
[Forsythia x intermedia] gi|6694693|gb|AAF25357
Length = 187
Score = 29.9 bits (64), Expect = 0.46
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +2
Query: 17 AFEFHGILQKGDRVRNAKSTAIAAVSG 97
+F FH IL GD V NA S AI + G
Sbjct: 44 SFYFHDILYDGDNVANATSAAIVSPPG 70
>At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical to
DNA Helicase [Arabidopsis thaliana] GI:11121449
Length = 1188
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -1
Query: 146 HLHVHPSPEFSRSAESIRTPPLLRCSSRSE 57
H H +P+ S S S+RTPP+ R +SR E
Sbjct: 229 HYHSTSTPQPSVSNFSLRTPPVDRSASRLE 258
>At1g72520.1 68414.m08386 lipoxygenase, putative similar to
lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140
[Lycopersicon esculentum], GB:CAB56692 [Arabidopsis
thaliana]
Length = 926
Score = 27.5 bits (58), Expect = 2.5
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -1
Query: 104 ESIRTPPLLRCSSRSEPYLPSVGFHGTRTLRQK 6
+S + P R ++PYLPS G RTLR+K
Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247
>At5g51590.1 68418.m06396 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 419
Score = 27.1 bits (57), Expect = 3.3
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +2
Query: 218 GKGSRQIGSVTSDKDWL*GPGRVGFGREADAAGAG 322
G+GSR+ + +D +WL P F +G G
Sbjct: 134 GQGSREPNNNNNDNNWLKNPQMFEFNNNTPTSGGG 168
>At2g29690.1 68415.m03609 anthranilate synthase, alpha subunit,
component I-2 (ASA2) identical to SP|P32069
Length = 621
Score = 26.6 bits (56), Expect = 4.3
Identities = 27/89 (30%), Positives = 41/89 (46%), Gaps = 2/89 (2%)
Frame = -1
Query: 332 IVQTRHR-PHPLPVQTRHAPV-LRANPYPKLRIQFADFPSLHYSID*RLFTLETCCGYGY 159
I T HR PHP + P+ L++ P L + A LH+S RL +++ C Y
Sbjct: 19 IAVTHHRTPHPPHFPSLRFPLSLKSPPATSLNL-VAGSKLLHFSR--RLPSIK--CSY-- 71
Query: 158 EPARHLHVHPSPEFSRSAESIRTPPLLRC 72
P+ L +F +++E PL RC
Sbjct: 72 TPSLDLSEEQFTKFKKASEKGNLVPLFRC 100
>At1g50410.1 68414.m05650 SNF2 domain-containing protein / helicase
domain-containing protein / RING finger
domain-containing protein similar to transcription
factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930;
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 981
Score = 26.6 bits (56), Expect = 4.3
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = -2
Query: 211 ILSTRGSSPWRPAADMGTNRRDISTYIPHLNFQGPQRVSGHRR 83
I TR P G++RRD+ST PH +GH +
Sbjct: 15 IQETRTRPQHPPRIAEGSHRRDLSTLRPHFLSGSSSGANGHTK 57
>At4g00270.1 68417.m00034 DNA-binding storekeeper protein-related
contains similarity to storekeeper protein [Solanum
tuberosum] gi|14268476|emb|CAC39398; contains PF04504:
Protein of unknown function, DUF573
Length = 302
Score = 26.2 bits (55), Expect = 5.7
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
Frame = +3
Query: 147 SRRFVPIS--AAGLQGEEPLVDRIM*GREVGKLDP*LRIRIGSEDRGVSGLDG 299
S R +P S AA + EE + ++ ++ KLDP L +RI +E+ +S L G
Sbjct: 27 SPRNIPSSKRAASVAEEETMKKKMKMKKKKKKLDPPLIVRIWNEEDELSILKG 79
>At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 676
Score = 26.2 bits (55), Expect = 5.7
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -1
Query: 65 RSEPYLPSVGFHGTRTLRQKR 3
R+ PY+P + F G RTL+ +R
Sbjct: 288 RTFPYVPDMKFSGNRTLKLER 308
>At1g78310.1 68414.m09126 VQ motif-containing protein contains
PF05678: VQ motif
Length = 311
Score = 26.2 bits (55), Expect = 5.7
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 155 PARHLHVHPSPEFSRSAESIRTPPLLRCSSR 63
P + +H HP P+ S IR PPL+ +R
Sbjct: 110 PQQPIH-HPKPQQSSRLHRIRPPPLVHVINR 139
>At4g21800.2 68417.m03154 ATP-binding family protein contains Pfam
domain, PF03029: Conserved hypothetical ATP binding
protein
Length = 379
Score = 25.8 bits (54), Expect = 7.6
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -2
Query: 274 SSEPILIRSYGSNLPTSLPYIILSTRGSSP 185
+S I+ ++ S PT + Y++ + R SSP
Sbjct: 156 ASGAIITEAFASTFPTVVTYVVDTPRSSSP 185
>At4g21800.1 68417.m03153 ATP-binding family protein contains Pfam
domain, PF03029: Conserved hypothetical ATP binding
protein
Length = 379
Score = 25.8 bits (54), Expect = 7.6
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = -2
Query: 274 SSEPILIRSYGSNLPTSLPYIILSTRGSSP 185
+S I+ ++ S PT + Y++ + R SSP
Sbjct: 156 ASGAIITEAFASTFPTVVTYVVDTPRSSSP 185
>At2g24980.1 68415.m02987 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 559
Score = 25.8 bits (54), Expect = 7.6
Identities = 21/93 (22%), Positives = 34/93 (36%), Gaps = 3/93 (3%)
Frame = -1
Query: 320 RHRPHPLPVQTRHAPVLRANPYPKLRIQFADFPSLHYSID*RLFTLETCCGYGYEPARHL 141
++ PHP P P P PK+ + P ++ S ++ Y P ++
Sbjct: 44 KYAPHPKPYVKSSPPPQYYTPSPKVNYKSPPPPYVYSSPPPPYYSPSPKVDYKSPPPPYV 103
Query: 140 HVHPSPEF---SRSAESIRTPPLLRCSSRSEPY 51
+ P P + S + PP SS PY
Sbjct: 104 YSSPPPPYYSPSPKVDYKSPPPPYVYSSPPPPY 136
>At2g16405.1 68415.m01878 transducin family protein / WD-40 repeat
family protein contains 7 WD-40 repeats (PF00400);
similar to WD-repeat protein 13 (SP:Q9H1Z4) [Homo
sapiens]
Length = 482
Score = 25.8 bits (54), Expect = 7.6
Identities = 17/45 (37%), Positives = 21/45 (46%)
Frame = -1
Query: 176 CCGYGYEPARHLHVHPSPEFSRSAESIRTPPLLRCSSRSEPYLPS 42
C G GY R+ P R E++RTP LL S +LPS
Sbjct: 68 CNGKGYVAYRNFISKP-----RKWENLRTPSLLSSPGNSGRWLPS 107
>At5g06780.1 68418.m00766 emsy N terminus domain-containing protein
/ ENT domain-containing protein contains Pfam profile
PF03735: ENT domain
Length = 320
Score = 25.4 bits (53), Expect = 10.0
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 2/27 (7%)
Frame = -1
Query: 164 GYEPARH--LHVHPSPEFSRSAESIRT 90
G + RH L VHPSP FS S + +T
Sbjct: 80 GNQVVRHQSLDVHPSPTFSASRKKQKT 106
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 25.4 bits (53), Expect = 10.0
Identities = 12/41 (29%), Positives = 19/41 (46%)
Frame = +2
Query: 23 EFHGILQKGDRVRNAKSTAIAAVSGYSLRTLKIQVRDVRGD 145
E +G L GD V NA+ + + + K+ D+ GD
Sbjct: 124 ETYGSLNNGDHVENAEESLAGRIMSKRSSSSKLFFYDLHGD 164
>At2g04220.1 68415.m00407 hypothetical protein
Length = 307
Score = 25.4 bits (53), Expect = 10.0
Identities = 19/54 (35%), Positives = 22/54 (40%), Gaps = 3/54 (5%)
Frame = -2
Query: 328 SRPGTGRIRFPSKPDTPRSSEPILIR---SYGSNLPTSLPYIILSTRGSSPWRP 176
S PGTG F KP T S+ G TS ST+ S+PW P
Sbjct: 242 SMPGTGHGLFIFKPGTTEDSDMEGSGHGGGGGGESDTSTGSRYYSTKSSNPWPP 295
>At1g03475.1 68414.m00329 coproporphyrinogen III oxidase, putative /
coproporphyrinogenase, putative / coprogen oxidase,
putative similar to coproporphyrinogen III oxidase,
chloroplast [precursor] from Glycine max [SP|P35055],
Nicotiana tabacum [SP|Q42946], Hordeum vulgare
[SP|Q42840], ESTs gb|AA586260 and dbj|D48620; contains
Pfam domain coproporphyrinogen III oxidase, aerobic
[PF01218]
Length = 362
Score = 25.4 bits (53), Expect = 10.0
Identities = 14/38 (36%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
Frame = -1
Query: 152 ARHLHVHPSPEFSRSAESIRTPP--LLRCSSRSEPYLP 45
+ LH P+P R + IR P LRCS E +P
Sbjct: 19 SHRLHYSPNPSTLRFSRPIRNKPNLALRCSVSIEKEVP 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,022,795
Number of Sequences: 28952
Number of extensions: 166338
Number of successful extensions: 545
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 545
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 409426656
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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