BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0976 (660 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 139 4e-34 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 139 4e-34 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 139 4e-34 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 44 2e-05 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.084 SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|ch... 28 1.4 SPAC29B12.13 |||carbon-sulfur lyase |Schizosaccharomyces pombe|c... 27 2.4 SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 25 7.3 SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 7.3 SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 25 7.3 SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 25 9.7 SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 9.7 SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 9.7 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 139 bits (336), Expect = 4e-34 Identities = 63/88 (71%), Positives = 73/88 (82%) Frame = +3 Query: 3 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 182 RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378 Query: 183 RRTGKSTEVNPKSIKSGDAAIVNLYLPS 266 RR+GK E +PK +KSGDA I + +PS Sbjct: 379 RRSGKKIEESPKFVKSGDACIAKM-VPS 405 Score = 70.9 bits (166), Expect = 1e-13 Identities = 31/39 (79%), Positives = 37/39 (94%) Frame = +2 Query: 248 QLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 364 ++VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 401 KMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 139 bits (336), Expect = 4e-34 Identities = 63/88 (71%), Positives = 73/88 (82%) Frame = +3 Query: 3 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 182 RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378 Query: 183 RRTGKSTEVNPKSIKSGDAAIVNLYLPS 266 RR+GK E +PK +KSGDA I + +PS Sbjct: 379 RRSGKKIEESPKFVKSGDACIAKM-VPS 405 Score = 70.9 bits (166), Expect = 1e-13 Identities = 31/39 (79%), Positives = 37/39 (94%) Frame = +2 Query: 248 QLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 364 ++VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 401 KMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 139 bits (336), Expect = 4e-34 Identities = 63/88 (71%), Positives = 73/88 (82%) Frame = +3 Query: 3 RRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVD 182 RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+D Sbjct: 319 RRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKID 378 Query: 183 RRTGKSTEVNPKSIKSGDAAIVNLYLPS 266 RR+GK E +PK +KSGDA I + +PS Sbjct: 379 RRSGKKIEESPKFVKSGDACIAKM-VPS 405 Score = 70.9 bits (166), Expect = 1e-13 Identities = 31/39 (79%), Positives = 37/39 (94%) Frame = +2 Query: 248 QLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 364 ++VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV Sbjct: 401 KMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 44.0 bits (99), Expect = 2e-05 Identities = 23/64 (35%), Positives = 34/64 (53%) Frame = +3 Query: 9 GYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRR 188 GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ Sbjct: 544 GYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDKT 602 Query: 189 TGKS 200 KS Sbjct: 603 NRKS 606 Score = 38.7 bits (86), Expect = 7e-04 Identities = 17/41 (41%), Positives = 28/41 (68%), Gaps = 1/41 (2%) Frame = +2 Query: 248 QLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 367 +L P+C+E F+++ +GRF +RD TVAVG V+K ++ Sbjct: 622 ELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 31.9 bits (69), Expect = 0.084 Identities = 15/31 (48%), Positives = 20/31 (64%) Frame = +2 Query: 266 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 358 PLC+ +E P LGRF +R TVA G++K Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589 >SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 312 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/36 (36%), Positives = 17/36 (47%) Frame = +3 Query: 456 FSYNCDTSFTERCFEGKKATNSFLFYIFYKACNVTL 563 F + C + CF +K FLFY F AC V + Sbjct: 139 FDHYCSFVGKDVCFSNQKFFYQFLFYGFSAACMVLI 174 >SPAC29B12.13 |||carbon-sulfur lyase |Schizosaccharomyces pombe|chr 1|||Manual Length = 137 Score = 27.1 bits (57), Expect = 2.4 Identities = 15/38 (39%), Positives = 20/38 (52%) Frame = +3 Query: 456 FSYNCDTSFTERCFEGKKATNSFLFYIFYKACNVTLFY 569 FS S +E GK A++SF IF K C TL++ Sbjct: 43 FSCTQKPSVSENENVGKYASSSFAERIFCKNCGTTLYF 80 >SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 425 Score = 25.4 bits (53), Expect = 7.3 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = +3 Query: 570 NLYKVIHNISETFCYDCKLKYKFIE*THLQ 659 N+Y++I +E Y C+L Y E +Q Sbjct: 245 NIYQIIEGAAENAKYICRLAYGLFEAPEIQ 274 >SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 268 Score = 25.4 bits (53), Expect = 7.3 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -3 Query: 244 MAASPDLMDFGLTSVDLPVRRSTFS 170 ++ +PDL D L+SVD P++ +T S Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51 >SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 987 Score = 25.4 bits (53), Expect = 7.3 Identities = 19/70 (27%), Positives = 28/70 (40%), Gaps = 6/70 (8%) Frame = +3 Query: 453 NFSYNCDTSFTERCFEGKKATN------SFLFYIFYKACNVTLFYNLYKVIHNISETFCY 614 NFS NC T + C KK T SF YK ++ F L ++ ++ Y Sbjct: 795 NFS-NCFTYWIINCILEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPIY 853 Query: 615 DCKLKYKFIE 644 YK ++ Sbjct: 854 RLHAAYKLVK 863 >SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = +3 Query: 210 NPKSIKSGDAAIVNLYLPSLYV*SPSRNSHPSVV 311 +P+ IKS +++ S+YV SP + + P +V Sbjct: 172 SPEIIKSATVQDAEIHISSIYVISPIKKNLPFLV 205 >SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/37 (27%), Positives = 19/37 (51%) Frame = -1 Query: 423 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 313 P + ++ T+PP S+ T + PT ++ + TA Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109 >SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Schizosaccharomyces pombe|chr 2|||Manual Length = 388 Score = 25.0 bits (52), Expect = 9.7 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = -1 Query: 396 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 274 + L P +S + TP+AT S+ + P GG D+T Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,695,967 Number of Sequences: 5004 Number of extensions: 53033 Number of successful extensions: 153 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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