BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0840 (1002 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 127 2e-31 AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 127 2e-31 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 122 7e-30 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 119 4e-29 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 27 0.35 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.9 AB161181-1|BAD08343.1| 933|Apis mellifera metabotropic glutamat... 24 1.9 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 24 2.5 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.3 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 127 bits (306), Expect = 2e-31 Identities = 66/104 (63%), Positives = 69/104 (66%) Frame = +1 Query: 370 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIELPVPVNSKLVSLR 549 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI + + Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72 Query: 550 TVKXXXXXXXXXXXXXXXXXXXXNKMDSTEPPYSEPRFEEIKKE 681 + NKMDSTEPPYSE RFEEIKKE Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 116 Score = 85.0 bits (201), Expect = 9e-19 Identities = 50/99 (50%), Positives = 59/99 (59%), Gaps = 1/99 (1%) Frame = +3 Query: 510 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIRRSKQNGFH*TTIQ*AQI*GNQEGKYP 689 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLI N T ++ + K Sbjct: 60 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLI--VGVNKMDSTEPPYSETRFEEIKKEV 117 Query: 690 HT-SRRLATTQLLSLSCPFSGWHGDTCWEPSTKMPWVQG 803 + +++ P SGWHGD E S+KMPW +G Sbjct: 118 SSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 156 Score = 48.4 bits (110), Expect = 1e-07 Identities = 29/58 (50%), Positives = 37/58 (63%) Frame = +2 Query: 674 RRKVSSYIKKIGYNPAAVAFVPIFWMARRHMLGAFNQNALGSRGGQVXRKGRAKLDGK 847 +++VSSYIKKIGYNPAAVAFVPI +ML + +G V RK K++GK Sbjct: 114 KKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EVSSKMPWFKGWTVERK-EGKVEGK 169 Score = 27.5 bits (58), Expect = 0.20 Identities = 11/11 (100%), Positives = 11/11 (100%) Frame = +2 Query: 335 WVLDKLKAERE 367 WVLDKLKAERE Sbjct: 1 WVLDKLKAERE 11 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 127 bits (306), Expect = 2e-31 Identities = 66/104 (63%), Positives = 69/104 (66%) Frame = +1 Query: 370 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIELPVPVNSKLVSLR 549 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI + + Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129 Query: 550 TVKXXXXXXXXXXXXXXXXXXXXNKMDSTEPPYSEPRFEEIKKE 681 + NKMDSTEPPYSE RFEEIKKE Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 173 Score = 85.0 bits (201), Expect = 9e-19 Identities = 50/99 (50%), Positives = 59/99 (59%), Gaps = 1/99 (1%) Frame = +3 Query: 510 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIRRSKQNGFH*TTIQ*AQI*GNQEGKYP 689 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLI N T ++ + K Sbjct: 117 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLI--VGVNKMDSTEPPYSETRFEEIKKEV 174 Query: 690 HT-SRRLATTQLLSLSCPFSGWHGDTCWEPSTKMPWVQG 803 + +++ P SGWHGD E S+KMPW +G Sbjct: 175 SSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 213 Score = 81.4 bits (192), Expect = 1e-17 Identities = 37/37 (100%), Positives = 37/37 (100%) Frame = +2 Query: 257 GGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 367 GGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE Sbjct: 32 GGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 68 Score = 64.9 bits (151), Expect = 1e-12 Identities = 29/30 (96%), Positives = 29/30 (96%) Frame = +3 Query: 165 MGKEKTHINIVVIGHVDSGKSTTTGHLIYK 254 MGKEK HINIVVIGHVDSGKSTTTGHLIYK Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYK 30 Score = 48.4 bits (110), Expect = 1e-07 Identities = 29/58 (50%), Positives = 37/58 (63%) Frame = +2 Query: 674 RRKVSSYIKKIGYNPAAVAFVPIFWMARRHMLGAFNQNALGSRGGQVXRKGRAKLDGK 847 +++VSSYIKKIGYNPAAVAFVPI +ML + +G V RK K++GK Sbjct: 171 KKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EVSSKMPWFKGWTVERK-EGKVEGK 226 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 122 bits (293), Expect = 7e-30 Identities = 63/104 (60%), Positives = 68/104 (65%) Frame = +1 Query: 370 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIELPVPVNSKLVSLR 549 GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLI + + Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129 Query: 550 TVKXXXXXXXXXXXXXXXXXXXXNKMDSTEPPYSEPRFEEIKKE 681 + NKMD T+PPYSE RFEEIKKE Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKE 173 Score = 85.0 bits (201), Expect = 9e-19 Identities = 50/100 (50%), Positives = 58/100 (58%), Gaps = 2/100 (2%) Frame = +3 Query: 510 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIRRSKQNGFH*TTIQ*AQI*GNQEGKYP 689 AAG GEFEAGISKNGQTREHALLAFTLGVKQLI + A+ +E K Sbjct: 117 AAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARF---EEIKKE 173 Query: 690 HTS--RRLATTQLLSLSCPFSGWHGDTCWEPSTKMPWVQG 803 +S +++ P SGWHGD EPS K PW +G Sbjct: 174 VSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKG 213 Score = 81.4 bits (192), Expect = 1e-17 Identities = 37/37 (100%), Positives = 37/37 (100%) Frame = +2 Query: 257 GGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 367 GGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE Sbjct: 32 GGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 68 Score = 64.9 bits (151), Expect = 1e-12 Identities = 29/30 (96%), Positives = 29/30 (96%) Frame = +3 Query: 165 MGKEKTHINIVVIGHVDSGKSTTTGHLIYK 254 MGKEK HINIVVIGHVDSGKSTTTGHLIYK Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYK 30 Score = 43.2 bits (97), Expect = 4e-06 Identities = 27/61 (44%), Positives = 36/61 (59%) Frame = +2 Query: 674 RRKVSSYIKKIGYNPAAVAFVPIFWMARRHMLGAFNQNALGSRGGQVXRKGRAKLDGKNX 853 +++VSSYIKKIGYN A+VAFVPI +ML + +G +V RK DGK Sbjct: 171 KKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEP-SPKTPWYKGWKVERKD-GNADGKTL 228 Query: 854 L 856 + Sbjct: 229 I 229 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 119 bits (287), Expect = 4e-29 Identities = 62/100 (62%), Positives = 65/100 (65%) Frame = +1 Query: 382 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIELPVPVNSKLVSLRTVKX 561 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI + + + Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 562 XXXXXXXXXXXXXXXXXXXNKMDSTEPPYSEPRFEEIKKE 681 NKMDSTEPPYSE RFEEIKKE Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 100 Score = 85.0 bits (201), Expect = 9e-19 Identities = 50/99 (50%), Positives = 59/99 (59%), Gaps = 1/99 (1%) Frame = +3 Query: 510 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLIRRSKQNGFH*TTIQ*AQI*GNQEGKYP 689 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLI N T ++ + K Sbjct: 44 AAGTGEFEAGISKNGQTREHALLAFTLGVKQLI--VGVNKMDSTEPPYSETRFEEIKKEV 101 Query: 690 HT-SRRLATTQLLSLSCPFSGWHGDTCWEPSTKMPWVQG 803 + +++ P SGWHGD E S+KMPW +G Sbjct: 102 SSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 140 Score = 48.4 bits (110), Expect = 1e-07 Identities = 29/58 (50%), Positives = 37/58 (63%) Frame = +2 Query: 674 RRKVSSYIKKIGYNPAAVAFVPIFWMARRHMLGAFNQNALGSRGGQVXRKGRAKLDGK 847 +++VSSYIKKIGYNPAAVAFVPI +ML + +G V RK K++GK Sbjct: 98 KKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EVSSKMPWFKGWTVERK-EGKVEGK 153 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 26.6 bits (56), Expect = 0.35 Identities = 12/29 (41%), Positives = 14/29 (48%) Frame = +1 Query: 421 VTIIDAPGHRDFIKNMITGTSQADCAVLI 507 VT +D PGH FI G D VL+ Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLV 223 Score = 25.4 bits (53), Expect = 0.81 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +3 Query: 177 KTHINIVVIGHVDSGKST 230 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.9 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +3 Query: 186 INIVVIGHVDSGKST 230 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >AB161181-1|BAD08343.1| 933|Apis mellifera metabotropic glutamate receptor protein. Length = 933 Score = 24.2 bits (50), Expect = 1.9 Identities = 8/14 (57%), Positives = 9/14 (64%) Frame = +3 Query: 810 RWXVREGPSWTGKM 851 RW R+G W GKM Sbjct: 2 RWINRDGEKWKGKM 15 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.8 bits (49), Expect = 2.5 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +1 Query: 406 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIE 510 T KYY D P + FIKN+ ++ +D LI+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLID 328 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.4 bits (48), Expect = 3.3 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = +1 Query: 406 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIE 510 T KYY D P + FIKN+ ++ +D L++ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLVD 328 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 283,770 Number of Sequences: 438 Number of extensions: 6528 Number of successful extensions: 38 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 34 length of database: 146,343 effective HSP length: 58 effective length of database: 120,939 effective search space used: 33258225 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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