BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0835 (561 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 50 2e-07 SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 50 3e-07 SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 41 1e-04 SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 35 0.009 SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 27 1.9 SPCC1223.12c |meu10||GPI anchored cell surface protein |Schizosa... 26 4.4 SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 26 4.4 SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 25 5.8 SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr... 25 7.6 SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po... 25 7.6 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 25 7.6 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 50.4 bits (115), Expect = 2e-07 Identities = 24/81 (29%), Positives = 44/81 (54%), Gaps = 1/81 (1%) Frame = +2 Query: 266 GDMDNFYEVLKDGTLLCKLANNIHPN-MIKKINTSSMAFKCMENINAFLEAARQLGVPAQ 442 G F + L++G +L L P+ +IK ++ + F+ +NIN FL+ +G+P Sbjct: 60 GPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGLPEI 119 Query: 443 ETFQTVDLWERQNLNSVVICL 505 F+ D++E +NL V+ C+ Sbjct: 120 FHFELTDIYEGKNLPKVIYCI 140 >SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein Stg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 174 Score = 49.6 bits (113), Expect = 3e-07 Identities = 28/69 (40%), Positives = 43/69 (62%), Gaps = 1/69 (1%) Frame = +2 Query: 293 LKDGTLLCKLANNIHPNMIKKINTSSMAFKCMENINAFLEAARQL-GVPAQETFQTVDLW 469 L+ G +LC++ I+ S+M F MENI+AF+ A+Q+ VP+Q+ FQT DL+ Sbjct: 31 LQSGVILCRICKEALGANIR-YKESNMPFVQMENISAFINYAQQVVHVPSQDMFQTSDLF 89 Query: 470 ERQNLNSVV 496 ER+N V+ Sbjct: 90 ERRNDEQVL 98 >SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 962 Score = 41.1 bits (92), Expect = 1e-04 Identities = 24/86 (27%), Positives = 42/86 (48%), Gaps = 1/86 (1%) Frame = +2 Query: 251 TKNTSGDMDNFYEVLKDGTLLCKLANNIHPNMIKKINTS-SMAFKCMENINAFLEAARQL 427 T N ++D+F + L +G +LC+LA +P + ++ + +NAF + Sbjct: 78 TNNEYQNLDDFVDALVNGKVLCQLAFKYYPKLASNWKPRYQISERNTVYLNAFFHFLDFI 137 Query: 428 GVPAQETFQTVDLWERQNLNSVVICL 505 G+ F+T DL R N+ V+ CL Sbjct: 138 GMFTPFRFETKDLVRRFNIPKVIYCL 163 >SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Manual Length = 614 Score = 34.7 bits (76), Expect = 0.009 Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 7/57 (12%) Frame = +2 Query: 281 FYEVLKDGTLLCKLANNIHPNMI------KKINTSSMA-FKCMENINAFLEAARQLG 430 F++ KDG +L KL N+ P+ I K+ N + FKC+EN N + +A+ +G Sbjct: 145 FFDQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNNVVINSAKAMG 201 Score = 30.3 bits (65), Expect = 0.20 Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 8/59 (13%) Frame = +2 Query: 278 NFYEVLKDGTLLCKLANNIHPNMI--KKINTS------SMAFKCMENINAFLEAARQLG 430 +F+ L+DG +L + + I PN + KK+N + M FK +EN N ++ + G Sbjct: 406 DFFNNLRDGLILLQAYDKITPNTVNWKKVNKAPASGDEMMRFKAVENCNYAVDLGKNQG 464 >SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 991 Score = 27.1 bits (57), Expect = 1.9 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = +2 Query: 350 KKINTSSMAFKCMENINAFLEAARQLGVPAQETFQTVDLWE 472 ++ +S A E + AF+E A+Q G+P E W+ Sbjct: 118 RRFRSSREAALKEEELQAFIEEAKQQGIPIDENATKKKSWD 158 >SPCC1223.12c |meu10||GPI anchored cell surface protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 416 Score = 25.8 bits (54), Expect = 4.4 Identities = 11/25 (44%), Positives = 19/25 (76%) Frame = +2 Query: 236 DDHRRTKNTSGDMDNFYEVLKDGTL 310 D+ R++K +S D D+F E+L +GT+ Sbjct: 352 DNPRQSKESSFDDDDFDELLGNGTI 376 >SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1562 Score = 25.8 bits (54), Expect = 4.4 Identities = 19/63 (30%), Positives = 24/63 (38%), Gaps = 1/63 (1%) Frame = +2 Query: 26 PVDPPACLHSVHSDFTFYRVCDLFK*FIKLLLVRNGKQ-PSDQIRICS*SPEKDQQQIQR 202 P PP L S H +FT F+ L R+ + S RI +P D QI Sbjct: 166 PPIPPEFLKSRHKEFTIPNPLQFISNFLSNLFSRDTRYLKSSHGRIIIINPYDDSSQIDE 225 Query: 203 GAG 211 G Sbjct: 226 RTG 228 >SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 879 Score = 25.4 bits (53), Expect = 5.8 Identities = 11/38 (28%), Positives = 18/38 (47%) Frame = -3 Query: 442 LCRYTQLSCGFEESVDILHALEGHRRCVDLFDHVRVDV 329 L R+ LS +D++ L+G + C HV D+ Sbjct: 67 LARWVHLSPSVHRCIDVVAFLQGQKFCFQNLVHVLQDI 104 >SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 25.0 bits (52), Expect = 7.6 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = -1 Query: 156 LIWSLGCLPFRTNNNLINYLNK 91 LIW+ CL T+ +LI YL++ Sbjct: 123 LIWNQWCLSHLTDEDLIAYLSR 144 >SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 25.0 bits (52), Expect = 7.6 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = -3 Query: 247 PVIIRIHSKDSWASSSLYLL 188 P IIR H +D W S + LL Sbjct: 331 PCIIRSHKEDDWVSDIVSLL 350 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 25.0 bits (52), Expect = 7.6 Identities = 8/19 (42%), Positives = 15/19 (78%) Frame = -1 Query: 525 SLSAQCCKQITTELRFCLS 469 +L+A+CC ++ + R+CLS Sbjct: 407 TLAAKCCVKLDAKYRWCLS 425 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,525,537 Number of Sequences: 5004 Number of extensions: 53130 Number of successful extensions: 176 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 236012634 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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