Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0826
(516 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13450.1 68416.m01692 2-oxoisovalerate dehydrogenase / 3-meth... 85 3e-17
At1g55510.1 68414.m06350 2-oxoisovalerate dehydrogenase, putativ... 83 8e-17
At5g50850.1 68418.m06300 pyruvate dehydrogenase E1 component bet... 39 0.002
At1g30120.1 68414.m03681 pyruvate dehydrogenase E1 component bet... 37 0.009
At2g34590.1 68415.m04250 transketolase family protein similar to... 36 0.016
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui... 30 1.1
At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase... 28 3.2
At2g02480.1 68415.m00187 DNA polymerase-related weak similarity ... 28 3.2
At2g03390.1 68415.m00298 uvrB/uvrC motif-containing protein cont... 27 7.5
At1g19140.2 68414.m02381 expressed protein 27 9.9
At1g19140.1 68414.m02380 expressed protein 27 9.9
>At3g13450.1 68416.m01692 2-oxoisovalerate dehydrogenase /
3-methyl-2-oxobutanoate dehydrogenase / branched-chain
alpha-keto acid dehydrogenase E1 beta subunit (DIN4)
identical to branched chain alpha-keto acid
dehydrogenase E1 beta subunit [Arabidopsis thaliana]
GI:7021286
Length = 358
Score = 85.0 bits (201), Expect = 3e-17
Identities = 36/61 (59%), Positives = 45/61 (73%)
Frame = +1
Query: 256 PLTSGFGAELAATVQEECFLHLEAPIARVTGWDAPFPHVFEPFYLPDKWRCYQALIQLIN 435
P+T GFGAE+AAT+ E CFL LEAP++RV G D PFP VFEPFY+P K + A+ +N
Sbjct: 298 PVTGGFGAEIAATIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVN 357
Query: 436 Y 438
Y
Sbjct: 358 Y 358
Score = 52.4 bits (120), Expect = 2e-07
Identities = 21/33 (63%), Positives = 29/33 (87%)
Frame = +2
Query: 158 CDVIDLQSILPWDEETVCNSVKKTGRCLISHEA 256
C++IDL++++PWD+E V SV+KTGR LISHEA
Sbjct: 265 CELIDLKTLIPWDKEIVETSVRKTGRLLISHEA 297
Score = 48.4 bits (110), Expect = 3e-06
Identities = 21/52 (40%), Positives = 35/52 (67%)
Frame = +3
Query: 3 AAEEVPVEDYTLPLGKAQTLRVGAAATLVGWGTQVHVLLEVADMARDKLGVT 158
A E+VP +DY +PL +A+ +R G+ TLVGWG Q+ ++E A + + G++
Sbjct: 214 AVEDVPEDDYMIPLSEAEVMREGSDITLVGWGAQL-TIMEQACLDAENEGIS 264
>At1g55510.1 68414.m06350 2-oxoisovalerate dehydrogenase, putative /
3-methyl-2-oxobutanoate dehydrogenase, putative /
branched-chain alpha-keto acid dehydrogenase E1 beta
subunit, putative strong similarity to branched chain
alpha-keto acid dehydrogenase E1 beta subunit
[Arabidopsis thaliana] GI:7021286; contains Pfam
profiles PF02779: Transketolase, pyridine binding
domain, PF02780: Transketolase, C-terminal domain
Length = 352
Score = 83.4 bits (197), Expect = 8e-17
Identities = 35/61 (57%), Positives = 45/61 (73%)
Frame = +1
Query: 256 PLTSGFGAELAATVQEECFLHLEAPIARVTGWDAPFPHVFEPFYLPDKWRCYQALIQLIN 435
P+T GFGAE++AT+ E CFL LEAP++RV G D PFP VFEPFY+P K + A+ +N
Sbjct: 292 PVTGGFGAEISATILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVN 351
Query: 436 Y 438
Y
Sbjct: 352 Y 352
Score = 56.0 bits (129), Expect = 1e-08
Identities = 24/33 (72%), Positives = 30/33 (90%)
Frame = +2
Query: 158 CDVIDLQSILPWDEETVCNSVKKTGRCLISHEA 256
C++IDL+++LPWD+ETV SVKKTGR LISHEA
Sbjct: 259 CELIDLKTLLPWDKETVEASVKKTGRLLISHEA 291
Score = 49.6 bits (113), Expect = 1e-06
Identities = 24/52 (46%), Positives = 34/52 (65%)
Frame = +3
Query: 3 AAEEVPVEDYTLPLGKAQTLRVGAAATLVGWGTQVHVLLEVADMARDKLGVT 158
A EEVP DY +PL +A+ +R G TLVGWG Q+ V +E A + +K G++
Sbjct: 208 AVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLTV-MEQACLDAEKEGIS 258
>At5g50850.1 68418.m06300 pyruvate dehydrogenase E1 component beta
subunit, mitochondrial / PDHE1-B (PDH2) identical to
SP|Q38799 Pyruvate dehydrogenase E1 component beta
subunit, mitochondrial precursor (EC 1.2.4.1) (PDHE1-B)
{Arabidopsis thaliana}
Length = 363
Score = 39.1 bits (87), Expect = 0.002
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +1
Query: 268 GFGAELAATVQEECFLHLEAPIARVTGWDAPFPH 369
G AE+ A+V EE F +L+AP+ R+ G D P P+
Sbjct: 303 GVCAEICASVVEESFSYLDAPVERIAGADVPMPY 336
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +3
Query: 6 AEEVPVEDYTLPLGKAQTLRVGAAATLVGWGTQVHVLLEVAD 131
+EE + LP+GKA+ R G T+V + V L+ A+
Sbjct: 216 SEEALDSSFCLPIGKAKIEREGKDVTIVTFSKMVGFALKAAE 257
>At1g30120.1 68414.m03681 pyruvate dehydrogenase E1 component beta
subunit, chloroplast identical to pyruvate dehydrogenase
E1 beta subunit [Arabidopsis thaliana] GI:2454184;
identical to cDNA pyruvate dehydrogenase E1 beta subunit
mRNA, nuclear gene encoding plastid protein GI:2454183
Length = 406
Score = 36.7 bits (81), Expect = 0.009
Identities = 17/31 (54%), Positives = 23/31 (74%)
Frame = +2
Query: 161 DVIDLQSILPWDEETVCNSVKKTGRCLISHE 253
+VID++S+ P+D T+ NSVKKT R LI E
Sbjct: 313 EVIDIRSLKPFDLHTIGNSVKKTHRVLIVEE 343
Score = 31.5 bits (68), Expect = 0.35
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 262 TSGFGAELAATVQEECFLHLEAPIARVTGWDAPFPH 369
T G GA L A + E +L+AP+ ++ D P P+
Sbjct: 347 TGGIGASLTAAINENFHDYLDAPVMCLSSQDVPTPY 382
>At2g34590.1 68415.m04250 transketolase family protein similar to
SP|O66113 Pyruvate dehydrogenase E1 component, beta
subunit (EC 1.2.4.1). {Zymomonas mobilis}; contains Pfam
profiles PF02779: Transketolase, pyridine binding
domain, PF02780: Transketolase, C-terminal domain
Length = 406
Score = 35.9 bits (79), Expect = 0.016
Identities = 17/31 (54%), Positives = 23/31 (74%)
Frame = +2
Query: 161 DVIDLQSILPWDEETVCNSVKKTGRCLISHE 253
+VID++S+ P+D T+ NSVKKT R LI E
Sbjct: 313 EVIDIRSLKPFDLYTIGNSVKKTHRVLIVEE 343
Score = 31.5 bits (68), Expect = 0.35
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 262 TSGFGAELAATVQEECFLHLEAPIARVTGWDAPFPH 369
T G GA L A + E +L+AP+ ++ D P P+
Sbjct: 347 TGGIGASLTAAINENFHDYLDAPVMCLSSQDVPTPY 382
>At4g38600.1 68417.m05464 HECT-domain-containing protein /
ubiquitin-transferase family protein similar to
SP|Q14669Thyroid receptor interacting protein 12
(TRIP12) {Homo sapiens}; contains Pfam profile PF00632:
HECT-domain (ubiquitin-transferase)
Length = 1888
Score = 29.9 bits (64), Expect = 1.1
Identities = 22/63 (34%), Positives = 31/63 (49%), Gaps = 7/63 (11%)
Frame = -2
Query: 323 SKCRKHSSCTVAASSA------PNPDVSGPRGISSTAQFSSRNCTR-SPRPRAGSTADR* 165
++ RK + T AA S+ P P SGP S A+ SS + + +P P + ST R
Sbjct: 3 TRSRKRAEATSAAPSSSSSSPPPPPSASGPTTRSKRARLSSSSSSSLAPTPPSSSTTTRS 62
Query: 164 RHS 156
R S
Sbjct: 63 RSS 65
>At4g15560.1 68417.m02377 1-deoxy-D-xylulose 5-phosphate synthase,
putative / 1-deoxyxylulose-5-phosphate synthase,
putative / DXP-synthase, putative (DEF) (CLA1) identical
to SP|Q38854 Probable 1-deoxy-D-xylulose 5-phosphate
synthase, chloroplast precursor (EC 4.1.3.37)
(1-deoxyxylulose-5-phosphate synthase) (DXP synthase)
(DXPS). [Mouse-ear cress] {Arabidopsis thaliana}, DEF
(deficient in photosynthesis) protein [Arabidopsis
thaliana] GI:1399261
Length = 717
Score = 28.3 bits (60), Expect = 3.2
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = +3
Query: 36 LPLGKAQTLRVGAAATLVGWGTQVHVLLEVADMARDK-LGVTVTSS 170
+ +GK + L+ G L+G+G+ V L A M ++ L VTV +
Sbjct: 575 IEIGKGRILKEGERVALLGYGSAVQSCLGAAVMLEERGLNVTVADA 620
>At2g02480.1 68415.m00187 DNA polymerase-related weak similarity to
DNA polymerase III holoenzyme tau subunit [Thermus
thermophilus] GI:2583049
Length = 1218
Score = 28.3 bits (60), Expect = 3.2
Identities = 17/43 (39%), Positives = 23/43 (53%)
Frame = -1
Query: 339 SSYRRLQVQKTFLLHGGGELGAEPRRERASWDIKHRPVFFTEL 211
+SYR + L G E G+ P R S+ K+RP+FF EL
Sbjct: 422 TSYRSQDGLEAVALDGEEEEGSTPETIR-SFSQKYRPMFFEEL 463
>At2g03390.1 68415.m00298 uvrB/uvrC motif-containing protein
contains Pfam profile PF02151: UvrB/uvrC motif
Length = 330
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = -1
Query: 324 LQVQKTFLLHGGGELGAEPRRERASWDIKHRPVFFTELHTVSSSQ 190
L+V+ + GGGE G +P ER+ + +FF +L + Q
Sbjct: 64 LRVEARWPFQGGGEQGLDPSSERSESANEDILIFFFQLDLATRVQ 108
>At1g19140.2 68414.m02381 expressed protein
Length = 312
Score = 26.6 bits (56), Expect = 9.9
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -2
Query: 272 NPDVSGPRGISSTAQFSSRNCTRSPRPRAGSTADR*RHSDAELVPRHV 129
NP+ SGP SST + S +PRA ++ R A L RHV
Sbjct: 58 NPNQSGPTASSSTGTGEGQRRHESRKPRAEFQEEQARVLSASL--RHV 103
>At1g19140.1 68414.m02380 expressed protein
Length = 311
Score = 26.6 bits (56), Expect = 9.9
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -2
Query: 272 NPDVSGPRGISSTAQFSSRNCTRSPRPRAGSTADR*RHSDAELVPRHV 129
NP+ SGP SST + S +PRA ++ R A L RHV
Sbjct: 58 NPNQSGPTASSSTGTGEGQRRHESRKPRAEFQEEQARVLSASL--RHV 103
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,883,421
Number of Sequences: 28952
Number of extensions: 186928
Number of successful extensions: 559
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 543
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 559
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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