BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0731 (624 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 118 7e-28 SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 28 1.3 SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 8.9 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.9 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 25 8.9 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 25 8.9 >SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 118 bits (284), Expect = 7e-28 Identities = 54/88 (61%), Positives = 66/88 (75%), Gaps = 1/88 (1%) Frame = +2 Query: 248 QGILHIALRNRQNKPILVNGKDVSTDVNAVLEHMKEFSDQVVSGQWKGYTGKAITDVINI 427 + + H ALRN KP+ VNG+DV V VL HMKEFSD V SG WKGYTGK I ++N+ Sbjct: 101 RAVFHPALRNVSEKPMKVNGQDVMPGVRKVLRHMKEFSDAVRSGAWKGYTGKPIKSIVNV 160 Query: 428 GIGGSDVGPLMVTEALKPYA-NHLKVHF 508 GIGGSD+GP+MVTEALKPY +L++HF Sbjct: 161 GIGGSDLGPVMVTEALKPYGQENLELHF 188 Score = 74.5 bits (175), Expect = 1e-14 Identities = 39/85 (45%), Positives = 51/85 (60%), Gaps = 2/85 (2%) Frame = +3 Query: 6 YYNENSTKIKMLQLFQQERERFEKNSLCIP--TPNDGDILLDYSKNRINSDVFKLLLDLA 179 +Y + + +LF ++ RFEK S DG IL D+SKN I + +LL+ LA Sbjct: 18 HYESVGKHLVLKELFAKDSSRFEKFSFTFNGLKEEDGPILFDFSKNLITEETVELLVKLA 77 Query: 180 KSRNVEQARDAMFAGQKINFTEDRA 254 K NVE RDA+FAG+ INFTEDRA Sbjct: 78 KEANVEGLRDALFAGEHINFTEDRA 102 Score = 69.7 bits (163), Expect = 3e-13 Identities = 32/40 (80%), Positives = 35/40 (87%) Frame = +1 Query: 505 FLSNIDGTHLAEVLKKLNPETALFIIASKTFTTQETITNA 624 F+SNIDGTHLAE LKK +PET LF+IASKTFTT ET TNA Sbjct: 188 FVSNIDGTHLAEALKKCDPETTLFLIASKTFTTAETCTNA 227 >SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 27.9 bits (59), Expect = 1.3 Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 2/54 (3%) Frame = +2 Query: 269 LRNRQN--KPILVNGKDVSTDVNAVLEHMKEFSDQVVSGQWKGYTGKAITDVIN 424 + NR N KP+ + KD + EH K+ D +SG G +++ IN Sbjct: 668 ITNRLNAKKPLFIESKDFGDNPLDTFEHYKQSVDAGLSGMLFSVIGGRLSEGIN 721 >SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1131 Score = 25.0 bits (52), Expect = 8.9 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 1/37 (2%) Frame = +3 Query: 180 KSRNVEQARDAMFAGQK-INFTEDRAYCI*LCVTVRT 287 K+ V++AR + +NF+EDR C LC V T Sbjct: 787 KADTVKRARQNNIKNKDDVNFSEDRKKCCALCGIVGT 823 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.0 bits (52), Expect = 8.9 Identities = 13/27 (48%), Positives = 15/27 (55%) Frame = -2 Query: 614 IVSCVVKVLDAMMNSAVSGFSFFKTSA 534 I S K + A NS +SGFS TSA Sbjct: 194 ISSADAKTVSASSNSTISGFSTSTTSA 220 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 25.0 bits (52), Expect = 8.9 Identities = 14/21 (66%), Positives = 15/21 (71%) Frame = +3 Query: 21 STKIKMLQLFQQERERFEKNS 83 STKI QL QQE E FEK+S Sbjct: 1273 STKI-FEQLTQQEEEEFEKDS 1292 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 25.0 bits (52), Expect = 8.9 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = +1 Query: 355 ILRSSSKRAMEGVYGKSYNRRDQHWYWRLRC 447 ++ S A E VYGK Y+R YW L C Sbjct: 357 VVGSPEYMAPEVVYGKKYDRTVD--YWSLGC 385 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,605,888 Number of Sequences: 5004 Number of extensions: 52888 Number of successful extensions: 159 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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