Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0731
(624 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_19976| Best HMM Match : PGI (HMM E-Value=0) 91 5e-19
SB_37337| Best HMM Match : PGI (HMM E-Value=0) 71 1e-12
SB_46637| Best HMM Match : Toxin_16 (HMM E-Value=1.6) 30 1.8
SB_15601| Best HMM Match : Toxin_16 (HMM E-Value=0.24) 30 1.8
SB_13681| Best HMM Match : Guanylate_cyc (HMM E-Value=2.8e-27) 28 7.1
SB_15087| Best HMM Match : RnaseH (HMM E-Value=4.8) 28 7.1
SB_16543| Best HMM Match : DUF872 (HMM E-Value=2.8) 27 9.4
>SB_19976| Best HMM Match : PGI (HMM E-Value=0)
Length = 664
Score = 91.5 bits (217), Expect = 5e-19
Identities = 41/87 (47%), Positives = 58/87 (66%)
Frame = +2
Query: 248 QGILHIALRNRQNKPILVNGKDVSTDVNAVLEHMKEFSDQVVSGQWKGYTGKAITDVINI 427
+ +LH ALR N + V GK++ ++ V + + F++ +VSG KGYT K TDV+NI
Sbjct: 67 RAVLHTALRADVNDEVYVEGKNIIPEIFEVKKKIDFFTNDIVSGIRKGYTNKPFTDVVNI 126
Query: 428 GIGGSDVGPLMVTEALKPYANHLKVHF 508
GIGGSD+GP M+ E L+ Y NHL +HF
Sbjct: 127 GIGGSDLGPAMIVEGLEYYKNHLNLHF 153
Score = 68.5 bits (160), Expect = 4e-12
Identities = 28/40 (70%), Positives = 36/40 (90%)
Frame = +1
Query: 505 FLSNIDGTHLAEVLKKLNPETALFIIASKTFTTQETITNA 624
F+SN+DG H+ E+LKK+NPET LF+I SKTFTTQET++NA
Sbjct: 153 FVSNVDGDHVNEILKKINPETTLFVIVSKTFTTQETLSNA 192
Score = 51.6 bits (118), Expect = 5e-07
Identities = 29/73 (39%), Positives = 45/73 (61%)
Frame = +3
Query: 36 MLQLFQQERERFEKNSLCIPTPNDGDILLDYSKNRINSDVFKLLLDLAKSRNVEQARDAM 215
M ++F Q+ ER EK + D ++DYSKN+I + LL+DLA N+++A +
Sbjct: 1 MKEMFFQDSERVEKFHI-----EWEDFVIDYSKNKITEETKGLLIDLANEINLKEAIEDY 55
Query: 216 FAGQKINFTEDRA 254
F+G+ IN TE+RA
Sbjct: 56 FSGEIINKTENRA 68
>SB_37337| Best HMM Match : PGI (HMM E-Value=0)
Length = 391
Score = 70.5 bits (165), Expect = 1e-12
Identities = 33/40 (82%), Positives = 37/40 (92%)
Frame = +1
Query: 505 FLSNIDGTHLAEVLKKLNPETALFIIASKTFTTQETITNA 624
F+SNIDGTHLA+ L +LNPET+LFIIASKTFTT ETITNA
Sbjct: 127 FVSNIDGTHLAKTLAELNPETSLFIIASKTFTTIETITNA 166
Score = 46.8 bits (106), Expect = 1e-05
Identities = 22/61 (36%), Positives = 38/61 (62%)
Frame = +3
Query: 3 EYYNENSTKIKMLQLFQQERERFEKNSLCIPTPNDGDILLDYSKNRINSDVFKLLLDLAK 182
+++ + + M +LFQ++ RFEK S + T +DG +L+DYSKN + + KLL L +
Sbjct: 27 QHHKSTALALHMRKLFQEDANRFEKFSTFLDT-SDGRLLVDYSKNIVTEETMKLLFRLDR 85
Query: 183 S 185
+
Sbjct: 86 A 86
Score = 39.1 bits (87), Expect = 0.003
Identities = 18/32 (56%), Positives = 22/32 (68%)
Frame = +2
Query: 248 QGILHIALRNRQNKPILVNGKDVSTDVNAVLE 343
+ +LHIALRNR N PILV+GKD V L+
Sbjct: 85 RAVLHIALRNRSNTPILVDGKDGPLMVTEALQ 116
Score = 33.1 bits (72), Expect = 0.19
Identities = 17/27 (62%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Frame = +2
Query: 431 IGGSDVGPLMVTEALKPYANH-LKVHF 508
+ G D GPLMVTEAL+PY L VHF
Sbjct: 102 VDGKD-GPLMVTEALQPYGKKGLNVHF 127
>SB_46637| Best HMM Match : Toxin_16 (HMM E-Value=1.6)
Length = 131
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G + +T +T + G G +VGP MVT
Sbjct: 68 EEVGQQMVTGDDRAFTCGPVTCCRSTGADGEEVGPQMVT 106
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 8 EEVGQQMVTGDDRGFTCCPVTCCRSTGADGEEVGRQMVT 46
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 38 EEVGRQMVTGDDRGFTCCPVTCCRSTGADGEEVGQQMVT 76
>SB_15601| Best HMM Match : Toxin_16 (HMM E-Value=0.24)
Length = 358
Score = 29.9 bits (64), Expect = 1.8
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G + +T +T + G G +VGP MVT
Sbjct: 295 EEVGQQMVTGDDRAFTCGPVTCCRSTGADGEEVGPQMVT 333
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 265 EEVGRQMVTGDDRGFTCGPVTCCRSTGADGEEVGQQMVT 303
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 25 EEVGRQMVTGDDRGFTCGPVTCCRSTGADGEEVGRQMVT 63
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 55 EEVGRQMVTGDDRGFTCGPVTCCRSTGADGEEVGRQMVT 93
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 85 EEVGRQMVTGDDRGFTCGPVTCCRSTGADGEEVGRQMVT 123
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 235 EEVGRQMVTGDDRGFTCGPVTCCRSTGADGEEVGRQMVT 273
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 115 EEVGRQMVTGDDRGFTCCPVTCCRSTGADGEEVGRQMVT 153
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 145 EEVGRQMVTGDDRGFTCCPVTYCRSTGADGEEVGRQMVT 183
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 350 KEFSDQVVSGQWKGYTGKAITDVINIGIGGSDVGPLMVT 466
+E Q+V+G +G+T +T + G G +VG MVT
Sbjct: 175 EEVGRQMVTGDDRGFTCCPVTCCRSTGADGEEVGRQMVT 213
>SB_13681| Best HMM Match : Guanylate_cyc (HMM E-Value=2.8e-27)
Length = 517
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = +3
Query: 84 LCIPTPNDGDILLDYSKNRINSDV-FKLLLDLAKSRNVEQARD 209
LCI +PN + D +K R+N+D K +++ + R Q+R+
Sbjct: 121 LCISSPNSTPVGKDLNKARLNTDKNIKSIINWPEGRQSLQSRE 163
>SB_15087| Best HMM Match : RnaseH (HMM E-Value=4.8)
Length = 326
Score = 27.9 bits (59), Expect = 7.1
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = -3
Query: 505 MDLKMISIGLQCFCDHE-RTHI*ASNTNVDHVCY 407
++LK I +GLQ C H THI + N V Y
Sbjct: 95 LELKAIKLGLQSLCSHMCNTHIKVLSDNQTAVAY 128
>SB_16543| Best HMM Match : DUF872 (HMM E-Value=2.8)
Length = 262
Score = 27.5 bits (58), Expect = 9.4
Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +1
Query: 49 SNKNGSVSKRTASVSQHRTM-ETSYLTIPRTV 141
S +G V + ASVS+HR S L IPRT+
Sbjct: 182 SETSGGVINQAASVSKHRLFRRLSSLAIPRTL 213
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,669,009
Number of Sequences: 59808
Number of extensions: 393300
Number of successful extensions: 1038
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 963
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1037
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1548368000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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