BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0448 (702 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces po... 29 0.49 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 28 1.1 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 26 4.5 SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 25 7.9 SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pomb... 25 7.9 SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schi... 25 7.9 >SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 374 Score = 29.5 bits (63), Expect = 0.49 Identities = 20/85 (23%), Positives = 37/85 (43%) Frame = -3 Query: 637 IILFINNTKLFPQTPTSQLIQHIWHHKYFSLLIDIFHTRHLFVRSVKY*IFRYTSLFLHF 458 I++ N +LF + LI+ ++H + + D +T F +F Y L L Sbjct: 22 IVVVTKNLELFKKYDDINLIKEAYNHVHKLIQKDERYTAVFFAHDST--VFSYLGLSLKA 79 Query: 457 LYVFILYVFECIPTLYVQHLSILSQ 383 Y Y+ + + +YV H +S+ Sbjct: 80 YYGMDYYLHKNVKAVYVIHTDWMSK 104 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +2 Query: 359 VTKEHREALAKNAKVLYIKCRDALKDIQNENIKKMKKQTG 478 V +EH L K+LY DAL ++ N K +++ TG Sbjct: 877 VIEEHNVLLLLAEKILYELPLDALDSVEQINSKSLRRVTG 916 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = +2 Query: 305 LNLNPQQDGTTLFVPVPKVTKEHREALAKNAKVL 406 L+L+ + G P+ +++ EH LAKN +L Sbjct: 1092 LSLHESRSGRVYMSPLTEISPEHLARLAKNTTLL 1125 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 25.4 bits (53), Expect = 7.9 Identities = 15/40 (37%), Positives = 21/40 (52%) Frame = -2 Query: 125 MFSKIRLHVFNSGTAAVFHLVYWYKFGENCHINFNFSALP 6 M+S I HVF+S A L+Y+ K G + +FS P Sbjct: 549 MYSWIYDHVFSSSDHAYESLIYYMKTGHK-QVVQSFSIFP 587 >SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pombe|chr 3|||Manual Length = 202 Score = 25.4 bits (53), Expect = 7.9 Identities = 20/63 (31%), Positives = 30/63 (47%) Frame = +2 Query: 275 EALKAISSSGLNLNPQQDGTTLFVPVPKVTKEHREALAKNAKVLYIKCRDALKDIQNENI 454 E K I S GL +Q + + K+ RE +NA Y+K + KDI +E + Sbjct: 108 ELAKPIMSKGLRSRTEQQES-----IGKLQDAIRELEEENAN--YVKAWNLRKDIFDETL 160 Query: 455 KKM 463 K+M Sbjct: 161 KQM 163 >SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = +2 Query: 386 AKNAKVLYIKCRDALKDIQNENIKKMKKQTGVSEDLIFNGT 508 +KN+ Y K A D +N NI K+ +++L N T Sbjct: 327 SKNSHSPYSKFSSAYPDAENSNISKINISIAGNKELYGNAT 367 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,921,050 Number of Sequences: 5004 Number of extensions: 60405 Number of successful extensions: 186 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 325165428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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