Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0396
(779 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 150 3e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 150 3e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 150 3e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 48 1e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 35 0.011
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.11
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.0
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.2
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 150 bits (363), Expect = 3e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +3
Query: 3 APANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 182
APA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A F
Sbjct: 279 APAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Query: 183 TAQVIVLNHPGQISNGYTPVLDCH 254
TAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCH 362
Score = 124 bits (299), Expect = 1e-29
Identities = 56/76 (73%), Positives = 66/76 (86%)
Frame = +2
Query: 257 AHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFA 436
AHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFA
Sbjct: 364 AHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFA 423
Query: 437 VRDMRQTVAVGVIKAV 484
VRDMRQTVAVGVIKAV
Sbjct: 424 VRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 150 bits (363), Expect = 3e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +3
Query: 3 APANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 182
APA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A F
Sbjct: 279 APAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Query: 183 TAQVIVLNHPGQISNGYTPVLDCH 254
TAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCH 362
Score = 124 bits (299), Expect = 1e-29
Identities = 56/76 (73%), Positives = 66/76 (86%)
Frame = +2
Query: 257 AHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFA 436
AHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFA
Sbjct: 364 AHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFA 423
Query: 437 VRDMRQTVAVGVIKAV 484
VRDMRQTVAVGVIKAV
Sbjct: 424 VRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 150 bits (363), Expect = 3e-37
Identities = 66/84 (78%), Positives = 74/84 (88%)
Frame = +3
Query: 3 APANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 182
APA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A F
Sbjct: 279 APAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF 338
Query: 183 TAQVIVLNHPGQISNGYTPVLDCH 254
TAQVI+LNHPGQIS GY+PVLDCH
Sbjct: 339 TAQVIILNHPGQISAGYSPVLDCH 362
Score = 124 bits (299), Expect = 1e-29
Identities = 56/76 (73%), Positives = 66/76 (86%)
Frame = +2
Query: 257 AHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFA 436
AHIACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFA
Sbjct: 364 AHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFA 423
Query: 437 VRDMRQTVAVGVIKAV 484
VRDMRQTVAVGVIKAV
Sbjct: 424 VRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 48.4 bits (110), Expect = 1e-06
Identities = 25/72 (34%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
Frame = +2
Query: 275 FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 454
FA++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD
Sbjct: 592 FAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGT 650
Query: 455 TVAVG-VIKAVN 487
TVAVG V+K ++
Sbjct: 651 TVAVGKVVKILD 662
Score = 41.9 bits (94), Expect = 1e-04
Identities = 25/84 (29%), Positives = 42/84 (50%), Gaps = 1/84 (1%)
Frame = +3
Query: 6 PANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADF 182
P N T EV ++ + E + ++ GD V V+ +++ GYV +KN P F
Sbjct: 503 PINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRF 560
Query: 183 TAQVIVLNHPGQISNGYTPVLDCH 254
AQ+ +L P ++ GY+ V+ H
Sbjct: 561 IAQIAILELPSILTTGYSCVMHIH 584
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +3
Query: 9 ANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 137
+++ T V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 292 SHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.11
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2
Query: 386 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 478
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1
Query: 122 QFLDGHVLYVETYIVSRYSFLESFV 48
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.2
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = -3
Query: 363 MAASPDLMDFGLTSVDLPVRRSTFS 289
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,210,429
Number of Sequences: 5004
Number of extensions: 66577
Number of successful extensions: 190
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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