BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0327 (733 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 108 7e-25 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 100 2e-22 SPBC1711.13 |his2||histidinol dehydrogenase His2 |Schizosaccharo... 32 0.073 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 31 0.17 SPCC338.08 |ctp1|mug38|sequence orphan|Schizosaccharomyces pombe... 28 1.6 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 27 2.1 SPBP23A10.06 |||manganese ion transporter |Schizosaccharomyces p... 27 3.6 SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 27 3.6 SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 26 4.8 SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 26 4.8 SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 26 6.4 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 26 6.4 SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc... 26 6.4 >SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 937 Score = 108 bits (260), Expect = 7e-25 Identities = 51/82 (62%), Positives = 63/82 (76%) Frame = +2 Query: 266 TPPPSDIVIARSQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDRLRNQRTGKYIV 445 TP PSDI IARSQ PK+IG+LA+EIG+ SE+ YG KAK++L +L RL ++R G Y+V Sbjct: 308 TPVPSDIEIARSQTPKNIGDLASEIGIAKSELEFYGSHKAKVNLEILQRLAHRRDGHYVV 367 Query: 446 VAGITPTPLGEGKSTTLIGLVK 511 V GITPTP GEGKST GLV+ Sbjct: 368 VTGITPTPFGEGKSTLTAGLVQ 389 Score = 97.5 bits (232), Expect = 2e-21 Identities = 50/89 (56%), Positives = 66/89 (74%), Gaps = 3/89 (3%) Frame = +3 Query: 3 WIKPGAVVIDCGINAIEDPTKKSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLMR 182 W+K G V ID GIN+I D TKKSG RL GD+ ++ A +VASA+TPVPG VGPMTVAML++ Sbjct: 222 WLKKGVVAIDVGINSIPDATKKSGYRLTGDIDFENAKEVASAITPVPGSVGPMTVAMLLQ 281 Query: 183 NTVQAA--RRQLDRLLAPS-WPLKPLRSP 260 N V++A R++ R P+ PLK L++P Sbjct: 282 NVVESAVRFRKMSRKRKPTLLPLK-LQTP 309 Score = 90.6 bits (215), Expect = 2e-19 Identities = 41/56 (73%), Positives = 45/56 (80%) Frame = +1 Query: 532 KNAFACMRQPSQGPTFGVKXXXXXXXYSQVIPMEEFNLHMTGDIHAVTAANNLLAA 699 K A AC+RQPSQGPTFG+K YSQ IPMEEFNLH+TGDIHA+TAA NLLAA Sbjct: 396 KLAIACVRQPSQGPTFGIKGGAAGGGYSQFIPMEEFNLHLTGDIHAITAATNLLAA 451 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 100 bits (240), Expect = 2e-22 Identities = 46/86 (53%), Positives = 64/86 (74%) Frame = +2 Query: 269 PPPSDIVIARSQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDRLRNQRTGKYIVV 448 P PSDI IA SQ+PK I LA E+G+ +E+ YG KAK++L+V +RL++++ G Y+VV Sbjct: 337 PVPSDIEIANSQEPKLISNLAKEMGIYDTELENYGNYKAKVNLAVYERLKHRKDGNYVVV 396 Query: 449 AGITPTPLGEGKSTTLIGLVKR*QHI 526 +GITPTP GEGKST + GLV+ H+ Sbjct: 397 SGITPTPFGEGKSTVVAGLVQAMGHL 422 Score = 93.1 bits (221), Expect = 4e-20 Identities = 42/59 (71%), Positives = 46/59 (77%) Frame = +1 Query: 523 HRGKNAFACMRQPSQGPTFGVKXXXXXXXYSQVIPMEEFNLHMTGDIHAVTAANNLLAA 699 H GK AC+RQPSQGPTFGVK Y+Q IPM++FNLHMTGDIHAVTAANNLL A Sbjct: 421 HLGKLGIACVRQPSQGPTFGVKGGAAGGGYAQFIPMDDFNLHMTGDIHAVTAANNLLVA 479 Score = 89.8 bits (213), Expect = 3e-19 Identities = 43/68 (63%), Positives = 56/68 (82%), Gaps = 1/68 (1%) Frame = +3 Query: 3 WIKPGAVVIDCGINAIEDPTKKSGQR-LVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLM 179 W+KPG+VV+D GINA++ ++G+R LVGDV ++ A KVAS++TPVPGGVGPMTVAMLM Sbjct: 253 WLKPGSVVVDVGINAVQ----RNGKRVLVGDVHFESASKVASSITPVPGGVGPMTVAMLM 308 Query: 180 RNTVQAAR 203 N V AA+ Sbjct: 309 ENIVNAAK 316 >SPBC1711.13 |his2||histidinol dehydrogenase His2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 32.3 bits (70), Expect = 0.073 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = -2 Query: 555 PHACKRVLPAMCCQRFTRPIRVVLLPSPSGVGVIPATTMYFPV 427 P + + + CQR +RPI V L P G V+P+T + V Sbjct: 107 PTIAVQTMRGVVCQRMSRPINRVGLYIPGGTAVLPSTALMLGV 149 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 31.1 bits (67), Expect = 0.17 Identities = 17/55 (30%), Positives = 25/55 (45%) Frame = +2 Query: 191 AGSAPTARPLACPLLASETASLTPITPPPSDIVIARSQKPKDIGELAAEIGLCPS 355 A ++ TA P + S+T + PITP S I + + PK + I C S Sbjct: 304 AAASATAPPQVTSPMVSQTQPVNPITPNYSSIPLPAERNPKTPQSFSTNIVQCAS 358 >SPCC338.08 |ctp1|mug38|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 285 Score = 27.9 bits (59), Expect = 1.6 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = -2 Query: 606 PSSSTPFNAKGRSLTRLPHACKRVLPAMCCQRFTRPIRVVL 484 PS + PF+ K +S+ PH+ LP F +PI V L Sbjct: 95 PSLNAPFSEKNQSVKIPPHS--PTLPVQNASAFVKPISVPL 133 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 27.5 bits (58), Expect = 2.1 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = +1 Query: 163 PWRCSCGTPCRQRADSSTACLPPLGL*NRFAHPDYATAQ 279 P + TP R + + T+ LPP+G +FA P A AQ Sbjct: 431 PQESATATPKRSPSATPTSALPPIG---KFAPPTTAKAQ 466 >SPBP23A10.06 |||manganese ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 26.6 bits (56), Expect = 3.6 Identities = 20/79 (25%), Positives = 36/79 (45%) Frame = +2 Query: 152 GAHDRGDAHAEHRAGSAPTARPLACPLLASETASLTPITPPPSDIVIARSQKPKDIGELA 331 GA G A+ + P +P+A L A + +T +T P D+V R Q + I + + Sbjct: 4 GAATEG-VEADLSVNAEPDVKPIAKMLSACVGSVITTLTVTPLDVVKTRLQS-ESISQYS 61 Query: 332 AEIGLCPSEVSQYGRTKAK 388 + + +++ GR K Sbjct: 62 STQPISSAKILGKGRPAPK 80 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 26.6 bits (56), Expect = 3.6 Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 3/45 (6%) Frame = -3 Query: 530 PRCAVNASPDLLEWCFYLHQVVSE---LYQQLRCISLCVGCVVCR 405 P NAS DL C+ H + E L ++RC C+ C C+ Sbjct: 474 PLNLANASSDL---CYVCHSALEEDCVLLGEIRCHIGCLSCTKCK 515 >SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 981 Score = 26.2 bits (55), Expect = 4.8 Identities = 19/67 (28%), Positives = 31/67 (46%) Frame = -2 Query: 522 CCQRFTRPIRVVLLPSPSGVGVIPATTMYFPVRWLRSLSRTDSEILALVRPY*DTSLGHR 343 C T P ++ +LP+ ++ T YF R+L L + + ++ LVR D + R Sbjct: 628 CALSRTIP-KLSILPTNGQYFLLTGATGYFGRRFLEYLVKLNISVVCLVRESSDEAAKER 686 Query: 342 PISAASS 322 IS S Sbjct: 687 LISLVPS 693 >SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual Length = 2410 Score = 26.2 bits (55), Expect = 4.8 Identities = 23/93 (24%), Positives = 39/93 (41%), Gaps = 3/93 (3%) Frame = +2 Query: 167 GDAHAEHRAGSAPTARPLACPLLASETASLTPITPP---PSDIVIARSQKPKDIGELAAE 337 G AEH G+ + L+ P + ++ S++P PP S+ + P G L E Sbjct: 1782 GSFDAEHAVGATFSQDDLSDPARSVDSDSVSPPLPPFVAGSNPNARNNNNPYFYGNLHTE 1841 Query: 338 IGLCPSEVSQYGRTKAKISLSVLDRLRNQRTGK 436 L + V G+ K SL+ ++ G+ Sbjct: 1842 SSLSLASVMS-GKEKRDFSLTRVEETFTDEDGQ 1873 >SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccharomyces pombe|chr 2|||Manual Length = 320 Score = 25.8 bits (54), Expect = 6.4 Identities = 9/23 (39%), Positives = 17/23 (73%) Frame = +3 Query: 150 VGPMTVAMLMRNTVQAARRQLDR 218 +G +T+AML+RN ++ QL++ Sbjct: 294 IGKVTIAMLLRNLIRLTSYQLNK 316 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.8 bits (54), Expect = 6.4 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = -3 Query: 545 ANAFFPRCAVNASPDLLEWCFYLHQVVSELYQQLR--CISLCVGCVVCRER 399 AN F S + + CFYL V++ LY+ +R L G +V RER Sbjct: 366 ANDFMQSEDYAKSEPIEDDCFYLDNVITPLYEFIRDQQFELLDGKLVRRER 416 >SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 636 Score = 25.8 bits (54), Expect = 6.4 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 3/50 (6%) Frame = +2 Query: 170 DAHAEHRAGSAPTARPLACPLLASETASLTPITPP---PSDIVIARSQKP 310 DA A S + +PL+ P+++S+ T + PP PS V + S KP Sbjct: 76 DAVASSSNVSLQSQQPLSKPVVSSKPNQTTAMPPPSNNPSRHVSSTSNKP 125 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,927,092 Number of Sequences: 5004 Number of extensions: 58500 Number of successful extensions: 188 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 187 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 345237368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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