Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0327
(733 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g12290.1 68416.m01534 tetrahydrofolate dehydrogenase/cyclohyd... 109 1e-24
At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 108 3e-24
At4g00600.1 68417.m00084 tetrahydrofolate dehydrogenase/cyclohyd... 103 2e-22
At2g38660.1 68415.m04748 tetrahydrofolate dehydrogenase/cyclohyd... 99 2e-21
At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase / 10-f... 96 2e-20
At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-conta... 32 0.45
At4g26420.1 68417.m03802 S-adenosyl-L-methionine:carboxyl methyl... 30 1.4
At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15) ... 30 1.4
At3g17465.1 68416.m02230 ribosomal protein L3 family protein 29 2.4
At2g27210.1 68415.m03270 kelch repeat-containing serine/threonin... 29 3.2
At5g12120.1 68418.m01423 ubiquitin-associated (UBA)/TS-N domain-... 29 4.2
At1g61130.1 68414.m06887 serine carboxypeptidase S10 family prot... 28 5.6
At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative s... 28 7.3
At5g54700.1 68418.m06812 ankyrin repeat family protein contains ... 28 7.3
At2g43270.1 68415.m05378 F-box family protein contains Pfam PF00... 27 9.7
At1g61680.1 68414.m06957 terpene synthase/cyclase family protein... 27 9.7
At1g11080.1 68414.m01269 serine carboxypeptidase S10 family prot... 27 9.7
>At3g12290.1 68416.m01534 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
(C1-THF synthase) [Includes: Methylenetetrahydrofolate
dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
contains Pfam profiles PF02882: Tetrahydrofolate
dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
catalytic domain
Length = 299
Score = 109 bits (263), Expect = 1e-24
Identities = 50/68 (73%), Positives = 56/68 (82%)
Frame = +3
Query: 3 WIKPGAVVIDCGINAIEDPTKKSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLMR 182
WIKPGA VID G NA+ DP+KKSG RLVGDV + EA KVA +TPVPGGVGPMTVAML+R
Sbjct: 228 WIKPGAAVIDVGTNAVSDPSKKSGYRLVGDVDFAEASKVAGFITPVPGGVGPMTVAMLLR 287
Query: 183 NTVQAARR 206
NTV A+R
Sbjct: 288 NTVDGAKR 295
>At4g00620.1 68417.m00086 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
(C1-THF synthase) [Includes: Methylenetetrahydrofolate
dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
contains Pfam profiles PF02882: Tetrahydrofolate
dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
catalytic domain
Length = 360
Score = 108 bits (260), Expect = 3e-24
Identities = 47/68 (69%), Positives = 58/68 (85%)
Frame = +3
Query: 3 WIKPGAVVIDCGINAIEDPTKKSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLMR 182
WIKPGAV+ID GIN +EDP+ G RLVGD+ Y+EA KVASA+TPVPGGVGPMT+AML+
Sbjct: 288 WIKPGAVLIDVGINPVEDPSAARGYRLVGDICYEEASKVASAITPVPGGVGPMTIAMLLS 347
Query: 183 NTVQAARR 206
NT+ +A+R
Sbjct: 348 NTLTSAKR 355
>At4g00600.1 68417.m00084 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
precursor (C1-THF synthase) [Includes:
Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
Formyltetrahydrofolate synthetase (EC 6.3.4.3)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
NAD(P)-binding domain, PF00763: Tetrahydrofolate
dehydrogenase/cyclohydrolase, catalytic domain
Length = 310
Score = 103 bits (246), Expect = 2e-22
Identities = 44/68 (64%), Positives = 56/68 (82%)
Frame = +3
Query: 3 WIKPGAVVIDCGINAIEDPTKKSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLMR 182
WIKPGAV+ID GI +EDP+ G+RLVGD+ Y EA K+ASA+TPVPG VGPMT+AML+
Sbjct: 238 WIKPGAVLIDVGIKPVEDPSAAGGERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLS 297
Query: 183 NTVQAARR 206
NT+ +A+R
Sbjct: 298 NTLTSAKR 305
>At2g38660.1 68415.m04748 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P09440 C-1-tetrahydrofolate synthase, mitochondrial
precursor (C1-THF synthase) [Includes:
Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9);
Formyltetrahydrofolate synthetase (EC 6.3.4.3)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF02882: Tetrahydrofolate dehydrogenase/cyclohydrolase,
NAD(P)-binding domain, PF00763: Tetrahydrofolate
dehydrogenase/cyclohydrolase, catalytic domain
Length = 352
Score = 99 bits (238), Expect = 2e-21
Identities = 44/68 (64%), Positives = 56/68 (82%)
Frame = +3
Query: 3 WIKPGAVVIDCGINAIEDPTKKSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLMR 182
W+KPGAVVID G +ED + + G RLVGDV Y+EA+ VASA+TPVPGGVGPMT+ ML+
Sbjct: 282 WLKPGAVVIDVGTTPVEDSSCEFGYRLVGDVCYEEALGVASAITPVPGGVGPMTITMLLC 341
Query: 183 NTVQAARR 206
NT++AA+R
Sbjct: 342 NTLEAAKR 349
>At1g50480.1 68414.m05660 formate--tetrahydrofolate ligase /
10-formyltetrahydrofolate synthetase (THFS) identical to
10-formyltetrahydrofolate synthetase (Arabidopsis
thaliana) GI:5921663
Length = 634
Score = 96.3 bits (229), Expect = 2e-20
Identities = 45/91 (49%), Positives = 60/91 (65%)
Frame = +2
Query: 233 LASETASLTPITPPPSDIVIARSQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDR 412
++S T L ++P P+DI IA S +P I E+A ++ + P YG+ KAK+ LS D
Sbjct: 1 MSSSTRKLEVVSPVPADIDIANSVEPLHISEIAKDLNINPLHYDLYGKYKAKVLLSAFDE 60
Query: 413 LRNQRTGKYIVVAGITPTPLGEGKSTTLIGL 505
L+ Q G Y+VV GITPTPLGEGKSTT +GL
Sbjct: 61 LQGQEDGYYVVVGGITPTPLGEGKSTTTVGL 91
Score = 95.1 bits (226), Expect = 4e-20
Identities = 42/64 (65%), Positives = 50/64 (78%)
Frame = +1
Query: 508 EALTAHRGKNAFACMRQPSQGPTFGVKXXXXXXXYSQVIPMEEFNLHMTGDIHAVTAANN 687
+AL A+ K C+RQPSQGPTFG+K YSQVIPM+EFNLH+TGDIHA+TA+NN
Sbjct: 93 QALGAYLDKKVVTCLRQPSQGPTFGIKGGAAGGGYSQVIPMDEFNLHLTGDIHAITASNN 152
Query: 688 LLAA 699
LLAA
Sbjct: 153 LLAA 156
>At2g26890.1 68415.m03226 DNAJ heat shock N-terminal domain-containing
protein contains Pfam profile PF00226: DnaJ domain
Length = 2554
Score = 31.9 bits (69), Expect = 0.45
Identities = 18/52 (34%), Positives = 23/52 (44%)
Frame = -1
Query: 280 TGRWRNRGERSGFRGQEGASKRSSCRRAACTVFRMSIATVMGPTPPGTGVTA 125
+G W + GF G GAS R C RA V+ T+ GP +TA
Sbjct: 1044 SGDWCDMSRLDGFGGGGGASVRELCARAMAIVYEQHYNTI-GPFEGTAHITA 1094
>At4g26420.1 68417.m03802 S-adenosyl-L-methionine:carboxyl
methyltransferase family protein similar to caffeine
synthase [Camellia sinensis][GI:9967143],
defense-related protein cjs1 [Brassica
carinata][GI:14009292],
S-adenosyl-L-methionine:salicylic acid carboxyl
methyltransferase [Clarkia breweri][GI:6002712]
Length = 619
Score = 30.3 bits (65), Expect = 1.4
Identities = 19/57 (33%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Frame = +3
Query: 3 WIKPGAVVIDCGINAIEDPTK-KSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVA 170
W K G V G+ I P K KSG+ +G + +E ++ V G GP T A
Sbjct: 532 WCKTGVVAKLMGLEMIPVPDKGKSGKDKLGTLLKRERLRRRERTLDVNGRTGPTTEA 588
>At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15)
almost identical to ubiquitin-specific protease 15
GI:11993475 [Arabidopsis thaliana], 7 amino acid
difference
Length = 924
Score = 30.3 bits (65), Expect = 1.4
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -1
Query: 313 FRLLRTSYYDVTGRWRNRGERSGFRGQEGASKRSSCRRAACTVFRMS 173
F L S D+T + +G +S + + R SCRR + TVF S
Sbjct: 217 FASLGISQNDITPQINTQGRKSVGKQHSSKANRESCRRDSATVFDSS 263
>At3g17465.1 68416.m02230 ribosomal protein L3 family protein
Length = 324
Score = 29.5 bits (63), Expect = 2.4
Identities = 15/47 (31%), Positives = 22/47 (46%), Gaps = 2/47 (4%)
Frame = -2
Query: 276 GGGVIGVSEAVSEAKRGQASGRA--VGALPARCSA*ASPRSWAPRRP 142
G +IG + EAK+G+ S R+ G + +C A W R P
Sbjct: 52 GSDIIGAQTRIIEAKQGEMSSRSKRTGIIAVKCGMTALWDKWGKRIP 98
>At2g27210.1 68415.m03270 kelch repeat-containing serine/threonine
phosphoesterase family protein similar to SP|P48482
Serine/threonine protein phosphatase PP1 isozyme 2 (EC
3.1.3.16) {Arabidopsis thaliana}; contains Pfam profile
PF00149: Calcineurin-like phosphoesterase
Length = 1006
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +2
Query: 239 SETASLTPITPPPSDIVIARSQKPK 313
SE+ASLTP PPPS + Q+P+
Sbjct: 40 SESASLTPSLPPPSQQQQQQQQQPQ 64
>At5g12120.1 68418.m01423 ubiquitin-associated (UBA)/TS-N
domain-containing protein contains Pfam profile PF00627:
UBA/TS-N domain
Length = 619
Score = 28.7 bits (61), Expect = 4.2
Identities = 21/51 (41%), Positives = 24/51 (47%)
Frame = -2
Query: 387 LALVRPY*DTSLGHRPISAASSPMSLGF*ERAITMSLGGGVIGVSEAVSEA 235
LA VRP P SAASSP R M+ G G +GV+ V EA
Sbjct: 540 LACVRPSQQYVAASSPYSAASSPYEAHMNGRTRMMTNGNG-MGVAMGVQEA 589
>At1g61130.1 68414.m06887 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II-3 precursor
(SP:P52711) (CP-MII.3. [Hordeum vulgare]
Length = 463
Score = 28.3 bits (60), Expect = 5.6
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 388 DFTVRSRQTTQPTHREIHRSCWYNSDTTW 474
D+ + T+R I +SC ++SDTTW
Sbjct: 229 DYAWNHAVVSDETYRVIKQSCNFSSDTTW 257
>At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative
similar to Swiss-Prot:Q9NG98 DNA topoisomerase III alpha
[Drosophila melanogaster]
Length = 926
Score = 27.9 bits (59), Expect = 7.3
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +2
Query: 83 SGRRGLPGGGQSSFGCDSCAGRRGA 157
SGRRG G G+ G S GRRG+
Sbjct: 861 SGRRGSRGRGRGGRGGQSSGGRRGS 885
>At5g54700.1 68418.m06812 ankyrin repeat family protein contains
ankyrin repeat domains, Pfam:PF00023
Length = 480
Score = 27.9 bits (59), Expect = 7.3
Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 3/57 (5%)
Frame = -1
Query: 202 RAACTVFRMSIATV---MGPTPPGTGVTAEATLTASW*ATSPTKRCPLFFVGSSIAL 41
R TV + IA+V +G PPG G+ E+T + + T +F+V +SIAL
Sbjct: 214 RNTITVVAILIASVTFAVGMNPPG-GIYQESTSSKGKSVAAKTVAFKIFYVSNSIAL 269
>At2g43270.1 68415.m05378 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 387
Score = 27.5 bits (58), Expect = 9.7
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 224 CPLLASETASLTPITPPPSDIVIARSQK 307
C +L ET + + PPP+DI + R +
Sbjct: 169 CEVLDVETGEWSELNPPPNDIDVGRKSR 196
>At1g61680.1 68414.m06957 terpene synthase/cyclase family protein
similar to 1,8-cineole synthase [GI:3309117][Salvia
officinalis]; contains Pfam profile: PF01397 terpene
synthase family
Length = 569
Score = 27.5 bits (58), Expect = 9.7
Identities = 11/27 (40%), Positives = 15/27 (55%), Gaps = 1/27 (3%)
Frame = -1
Query: 724 VEDARVHFVQQ-EDCWQRLRHECRQSC 647
V++AR H Q+ W+RL EC C
Sbjct: 505 VDEARTHVAQKISRAWKRLNRECLNPC 531
>At1g11080.1 68414.m01269 serine carboxypeptidase S10 family protein
similar to Serine carboxypeptidase II chains A and B
(SP:P08819) (EC 3.4.16.6) [Triticum aestivum (Wheat)]
Length = 492
Score = 27.5 bits (58), Expect = 9.7
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +1
Query: 424 THREIHRSCWYNSDTTW 474
THR I R+C ++SD TW
Sbjct: 262 THRIITRTCNFSSDNTW 278
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,119,336
Number of Sequences: 28952
Number of extensions: 337598
Number of successful extensions: 1092
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1041
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1091
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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