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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVm0318
         (644 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co...    54   6e-08
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si...    49   2e-06
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si...    44   1e-04
At3g19540.1 68416.m02477 expressed protein contains Pfam profile...    32   0.28 
At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id...    29   3.5  
At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing pro...    28   4.6  
At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi...    27   8.1  

>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
           contains Pfam PF00728: Glycosyl hydrolase family 20,
           catalytic domain; contains Pfam PF02838: Glycosyl
           hydrolase family 20, domain 2; similar to
           Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
           (N-acetyl-beta- glucosaminidase)
           (Beta-N-acetylhexosaminidase) (Hexosaminidase B)
           (Swiss-Prot:P07686) [Homo sapiens]
          Length = 535

 Score = 54.4 bits (125), Expect = 6e-08
 Identities = 27/66 (40%), Positives = 37/66 (56%), Gaps = 3/66 (4%)
 Frame = +2

Query: 2   CMWTEVVNDMNIMSRVWPRASAVAERLWGHESQAA---YQVYSRLEEHTCRMNARGIRAQ 172
           CMW E ++  +I   +WPRA+A AERLW   ++ A     V +RL    C +N RG+ A 
Sbjct: 457 CMWGEHIDASDIEQTIWPRAAAAAERLWTPYAKLAKNPNNVTTRLAHFRCLLNQRGVAAA 516

Query: 173 PPSGPG 190
           P  G G
Sbjct: 517 PLVGGG 522


>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase A SP:P13723 from
           [Dictyostelium discoideum]
          Length = 541

 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 26/79 (32%), Positives = 36/79 (45%), Gaps = 14/79 (17%)
 Frame = +2

Query: 2   CMWTEVVNDMNIMSRVWPRASAVAERLWG-----HESQAAYQVYSRLEEHTCRMNARGIR 166
           CMW E  +   ++  +WPRA+A AER+W       +         RL    C +N RG+ 
Sbjct: 460 CMWGETADTSVVLQTIWPRAAAAAERMWSTREAVSKGNITLTALPRLHYFRCLLNNRGVP 519

Query: 167 A---------QPPSGPGFC 196
           A         +PP GPG C
Sbjct: 520 AAPVDNFYARRPPLGPGSC 538


>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase precursor SP:P43077 from
           [Candida albicans]
          Length = 580

 Score = 43.6 bits (98), Expect = 1e-04
 Identities = 27/70 (38%), Positives = 38/70 (54%), Gaps = 5/70 (7%)
 Frame = +2

Query: 5   MWTEVVNDMNIMSRVWPRASAVAERLW-GHESQAAY----QVYSRLEEHTCRMNARGIRA 169
           +W+E  +   + SR+WPRASA+AE LW G+  +       +   RL     RM  RGI A
Sbjct: 496 LWSEQADSTVLDSRLWPRASALAESLWSGNRDERGVKRCGEAVDRLNLWRYRMVKRGIGA 555

Query: 170 QPPSGPGFCL 199
           +P   P +CL
Sbjct: 556 EPIQ-PLWCL 564


>At3g19540.1 68416.m02477 expressed protein contains Pfam profile
           PF04788: Protein of unknown function (DUF620)
          Length = 485

 Score = 32.3 bits (70), Expect = 0.28
 Identities = 16/41 (39%), Positives = 22/41 (53%)
 Frame = -1

Query: 209 TKRLGRSQGHWVAGRVCRARSFCMCAPLSESILDMLPVIRG 87
           +KRLG   GHWV G++ RA S    A    + L +L  + G
Sbjct: 98  SKRLGSGLGHWVKGQLSRAPSVAATAAYRRNDLRLLLGVMG 138


>At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly
            identical to auxin transport protein; BIG [Arabidopsis
            thaliana] GI:21779966; contains Pfam profiles PF02207:
            Putative zinc finger in N-recognin, PF00569: Zinc finger
            ZZ type
          Length = 5098

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 13/29 (44%), Positives = 16/29 (55%)
 Frame = -3

Query: 165  RMPRAFILHVCSSKREYT*YAACDSWPHS 79
            R  R   LH+C SK +Y  Y+  DSW  S
Sbjct: 3023 RYARRLFLHLCGSKTQY--YSVRDSWQFS 3049


>At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing
           protein contains Pfam profile PF01612: 3'-5' exonuclease
          Length = 239

 Score = 28.3 bits (60), Expect = 4.6
 Identities = 11/33 (33%), Positives = 17/33 (51%)
 Frame = +2

Query: 2   CMWTEVVNDMNIMSRVWPRASAVAERLWGHESQ 100
           C+W E     N+  R+W  +S V E +W   S+
Sbjct: 202 CIWKE---RSNVKERIWKESSNVKEHIWKESSK 231


>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
           histidine--tRNA ligase, putative similar to SP|P12081
           Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
           ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
           PF00587: tRNA synthetase class II core domain (G, H, P,
           S and T), PF03129: Anticodon binding domain
          Length = 479

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +2

Query: 59  ASAVAERLWGHESQAAYQVYSRLEEHTCRMNARGI 163
           A+ +  +LWG +  A Y V  R E+H  R    GI
Sbjct: 399 AAELVSQLWGAKINAEYLVSKRKEKHFNRAKESGI 433


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,731,045
Number of Sequences: 28952
Number of extensions: 237952
Number of successful extensions: 365
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 361
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 365
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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