BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0318 (644 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 54 6e-08 At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 49 2e-06 At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 44 1e-04 At3g19540.1 68416.m02477 expressed protein contains Pfam profile... 32 0.28 At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 29 3.5 At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing pro... 28 4.6 At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi... 27 8.1 >At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein contains Pfam PF00728: Glycosyl hydrolase family 20, catalytic domain; contains Pfam PF02838: Glycosyl hydrolase family 20, domain 2; similar to Beta-hexosaminidase beta chain precursor (EC 3.2.1.52) (N-acetyl-beta- glucosaminidase) (Beta-N-acetylhexosaminidase) (Hexosaminidase B) (Swiss-Prot:P07686) [Homo sapiens] Length = 535 Score = 54.4 bits (125), Expect = 6e-08 Identities = 27/66 (40%), Positives = 37/66 (56%), Gaps = 3/66 (4%) Frame = +2 Query: 2 CMWTEVVNDMNIMSRVWPRASAVAERLWGHESQAA---YQVYSRLEEHTCRMNARGIRAQ 172 CMW E ++ +I +WPRA+A AERLW ++ A V +RL C +N RG+ A Sbjct: 457 CMWGEHIDASDIEQTIWPRAAAAAERLWTPYAKLAKNPNNVTTRLAHFRCLLNQRGVAAA 516 Query: 173 PPSGPG 190 P G G Sbjct: 517 PLVGGG 522 >At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase A SP:P13723 from [Dictyostelium discoideum] Length = 541 Score = 49.2 bits (112), Expect = 2e-06 Identities = 26/79 (32%), Positives = 36/79 (45%), Gaps = 14/79 (17%) Frame = +2 Query: 2 CMWTEVVNDMNIMSRVWPRASAVAERLWG-----HESQAAYQVYSRLEEHTCRMNARGIR 166 CMW E + ++ +WPRA+A AER+W + RL C +N RG+ Sbjct: 460 CMWGETADTSVVLQTIWPRAAAAAERMWSTREAVSKGNITLTALPRLHYFRCLLNNRGVP 519 Query: 167 A---------QPPSGPGFC 196 A +PP GPG C Sbjct: 520 AAPVDNFYARRPPLGPGSC 538 >At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase precursor SP:P43077 from [Candida albicans] Length = 580 Score = 43.6 bits (98), Expect = 1e-04 Identities = 27/70 (38%), Positives = 38/70 (54%), Gaps = 5/70 (7%) Frame = +2 Query: 5 MWTEVVNDMNIMSRVWPRASAVAERLW-GHESQAAY----QVYSRLEEHTCRMNARGIRA 169 +W+E + + SR+WPRASA+AE LW G+ + + RL RM RGI A Sbjct: 496 LWSEQADSTVLDSRLWPRASALAESLWSGNRDERGVKRCGEAVDRLNLWRYRMVKRGIGA 555 Query: 170 QPPSGPGFCL 199 +P P +CL Sbjct: 556 EPIQ-PLWCL 564 >At3g19540.1 68416.m02477 expressed protein contains Pfam profile PF04788: Protein of unknown function (DUF620) Length = 485 Score = 32.3 bits (70), Expect = 0.28 Identities = 16/41 (39%), Positives = 22/41 (53%) Frame = -1 Query: 209 TKRLGRSQGHWVAGRVCRARSFCMCAPLSESILDMLPVIRG 87 +KRLG GHWV G++ RA S A + L +L + G Sbjct: 98 SKRLGSGLGHWVKGQLSRAPSVAATAAYRRNDLRLLLGVMG 138 >At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly identical to auxin transport protein; BIG [Arabidopsis thaliana] GI:21779966; contains Pfam profiles PF02207: Putative zinc finger in N-recognin, PF00569: Zinc finger ZZ type Length = 5098 Score = 28.7 bits (61), Expect = 3.5 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = -3 Query: 165 RMPRAFILHVCSSKREYT*YAACDSWPHS 79 R R LH+C SK +Y Y+ DSW S Sbjct: 3023 RYARRLFLHLCGSKTQY--YSVRDSWQFS 3049 >At2g36110.1 68415.m04434 3'-5' exonuclease domain-containing protein contains Pfam profile PF01612: 3'-5' exonuclease Length = 239 Score = 28.3 bits (60), Expect = 4.6 Identities = 11/33 (33%), Positives = 17/33 (51%) Frame = +2 Query: 2 CMWTEVVNDMNIMSRVWPRASAVAERLWGHESQ 100 C+W E N+ R+W +S V E +W S+ Sbjct: 202 CIWKE---RSNVKERIWKESSNVKEHIWKESSK 231 >At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / histidine--tRNA ligase, putative similar to SP|P12081 Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS) {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain Length = 479 Score = 27.5 bits (58), Expect = 8.1 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +2 Query: 59 ASAVAERLWGHESQAAYQVYSRLEEHTCRMNARGI 163 A+ + +LWG + A Y V R E+H R GI Sbjct: 399 AAELVSQLWGAKINAEYLVSKRKEKHFNRAKESGI 433 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,731,045 Number of Sequences: 28952 Number of extensions: 237952 Number of successful extensions: 365 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 361 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 365 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1334473344 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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