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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVm0299
         (640 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    50   3e-07
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    44   2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    40   4e-04
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   1.3  
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    27   2.3  
SPAC3G9.14 |sak1||transcriptional repressor Sak1|Schizosaccharom...    26   4.0  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   5.3  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 24/61 (39%), Positives = 37/61 (60%)
 Frame = -3

Query: 503 ECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKK 324
           E  K++DK+ NG +   ELTH L +LGE+L   EVA++ ++     D DG+I Y  F + 
Sbjct: 89  EAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DTDGDGVINYEEFSRV 146

Query: 323 V 321
           +
Sbjct: 147 I 147



 Score = 28.3 bits (60), Expect = 0.99
 Identities = 15/60 (25%), Positives = 31/60 (51%)
 Frame = -3

Query: 509 FLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFL 330
           F E   L+D++++G +   EL   + +LG+    +E+ ++  +     D +G I +  FL
Sbjct: 14  FREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEFL 71


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 43.6 bits (98), Expect = 2e-05
 Identities = 23/71 (32%), Positives = 41/71 (57%)
 Frame = -3

Query: 527 PGSV*RFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMI 348
           PG    F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K        DGM+
Sbjct: 73  PGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMV 129

Query: 347 PYAAFLKKVMA 315
            Y  F++ ++A
Sbjct: 130 NYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 19/65 (29%), Positives = 39/65 (60%)
 Frame = -3

Query: 509 FLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFL 330
           +++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP  + G   Y  F+
Sbjct: 80  YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TNSGSFDYYDFV 137

Query: 329 KKVMA 315
           +++MA
Sbjct: 138 QRIMA 142



 Score = 29.9 bits (64), Expect = 0.32
 Identities = 14/56 (25%), Positives = 32/56 (57%)
 Frame = -3

Query: 503 ECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 336
           E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 13  EAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = +1

Query: 163  APPDELSPPRALPAPVPQSRASVF*GPSHRTS 258
            APP    PP A P P+P S A     P  R++
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRSA 1751


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 13/41 (31%), Positives = 23/41 (56%)
 Frame = -3

Query: 503 ECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 381
           E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 41  EAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81


>SPAC3G9.14 |sak1||transcriptional repressor
           Sak1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 766

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 6/45 (13%)
 Frame = +1

Query: 163 APPDELSPPRALPAPVPQSRASVF*GPS------HRTSSQESYDL 279
           APP  +S P     P+PQ  +S   G S      H  S+QE ++L
Sbjct: 298 APPRSVSQPTYFSQPMPQFSSSFVPGTSSIVPTLHPASAQEDFNL 342


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = -3

Query: 428 LGEKLDDSEVAEVTKDCMDPED 363
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,220,737
Number of Sequences: 5004
Number of extensions: 38365
Number of successful extensions: 129
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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