Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVm0252
         (750 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26F1.02 |||pinin homologue|Schizosaccharomyces pombe|chr 1||...    34   0.019
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    32   0.076
SPBC19G7.13 |trf1||telomeric DNA binding factor Trf1|Schizosacch...    30   0.40 
SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces pom...    29   0.94 
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    28   1.2  
SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces pom...    28   1.6  
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb...    28   1.6  
SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa...    27   2.2  
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo...    27   2.9  
SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|c...    26   5.0  
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p...    26   6.6  
SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccha...    26   6.6  
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb...    25   8.7  
SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces po...    25   8.7  

>SPAC26F1.02 |||pinin homologue|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 197

 Score = 34.3 bits (75), Expect = 0.019
 Identities = 19/63 (30%), Positives = 39/63 (61%)
 Frame = -3

Query: 742 GTLGVHRQTRDREIRSRREPKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKHPPKI 563
           GTLG  +Q  +RE +S R+ KR + + ++L +R++Q+L+    ++ ++ E L  +   + 
Sbjct: 82  GTLGKFQQESEREQKSARKVKRAELE-EKLAKRREQELQELEKQEKIEAEILESRLQEQR 140

Query: 562 QVA 554
           +VA
Sbjct: 141 KVA 143


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 32.3 bits (70), Expect = 0.076
 Identities = 17/64 (26%), Positives = 38/64 (59%)
 Frame = -3

Query: 724 RQTRDREIRSRREPKRQDYDLKELKERQKQQLRHKALKKGLDPEALTGKHPPKIQVASKY 545
           ++  ++ ++  +E K+Q+ + ++ +E+QKQ+ R K LKK    EA   K   + ++  + 
Sbjct: 639 KKREEQRLKREQEKKQQELERQKREEKQKQKEREKKLKK-QQQEADREKMAREQRLREEE 697

Query: 544 ERRV 533
           E+R+
Sbjct: 698 EKRI 701


>SPBC19G7.13 |trf1||telomeric DNA binding factor
           Trf1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 485

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 4/75 (5%)
 Frame = -2

Query: 740 NSGSASSNSRPRNTISKRAKETGLRLKRAQRK---TKAATEAQSSQEGSRPRSA-HRQAP 573
           N  S S+   P N+  +R+K+ GLR K  + +   +++ ++   S     P S     + 
Sbjct: 314 NYSSISAIPIPANSFKRRSKKNGLRFKAGEAESSPSESGSDLTDSLAFGIPSSTFDGSSE 373

Query: 572 AQNSSSVQVREACRH 528
            QN SSV + +  RH
Sbjct: 374 TQNVSSVVLYDQVRH 388


>SPAC869.02c |||nitric oxide dioxygenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 28.7 bits (61), Expect = 0.94
 Identities = 13/35 (37%), Positives = 21/35 (60%)
 Frame = -3

Query: 730 VHRQTRDREIRSRREPKRQDYDLKELKERQKQQLR 626
           V+R+  D    +R+    + Y++KEL E QKQ +R
Sbjct: 6   VNRENADVANTNRQANLAEGYEIKELNESQKQYIR 40


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 22/80 (27%), Positives = 34/80 (42%), Gaps = 9/80 (11%)
 Frame = -2

Query: 740 NSGSASSNSRPRNTISKRAKETGLRLKRAQRKTKAATEAQSSQEGSRPRSA--------- 588
           +S S SS+S    T S ++  +      +   T ++T   SS   SRP S+         
Sbjct: 346 SSSSPSSSSFSSTTSSSKSSSSFSSTVSSSSSTSSSTLTSSSSSSSRPASSSSHSSSLSS 405

Query: 587 HRQAPAQNSSSVQVREACRH 528
           H+ + +  SSS  V  A  H
Sbjct: 406 HKSSSSSKSSSAPVSSAFYH 425


>SPAC17H9.06c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 601

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 17/47 (36%), Positives = 26/47 (55%)
 Frame = -2

Query: 743 RNSGSASSNSRPRNTISKRAKETGLRLKRAQRKTKAATEAQSSQEGS 603
           +N G+  SNS  R+  +K+ KE+    +   R T+  T+A SS E S
Sbjct: 32  KNKGAMKSNSNSRSRGNKKLKESQFSSRDNFRTTQ--TQASSSSEPS 76


>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 814

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 20/73 (27%), Positives = 30/73 (41%), Gaps = 1/73 (1%)
 Frame = -2

Query: 746 PRNSGSASSNSRPRNTISK-RAKETGLRLKRAQRKTKAATEAQSSQEGSRPRSAHRQAPA 570
           P     ASS     N+ SK RAK  G    +  +KT    E +   E ++P   H++   
Sbjct: 60  PIRKRRASSKKNMSNSSSKKRAKVMGNGGLKNGKKTAVVKEEEDFNEIAKPSPKHKRVSK 119

Query: 569 QNSSSVQVREACR 531
            N S    + A +
Sbjct: 120 ANGSKNGAKSAVK 132


>SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 707

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 13/34 (38%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
 Frame = -3

Query: 724 RQTRDREIRSRREPKRQDYDLKE-LKERQKQQLR 626
           RQTR+ + +  RE  +QD + KE +++++KQ L+
Sbjct: 655 RQTREEQRKLIRESVQQDQEHKEQMRQKKKQALK 688


>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1336

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = -3

Query: 91   TKYFYYLYFSTKRR*QPMTIQ*TNLFHK 8
            ++ F Y+Y ST+R  Q + I  T++ HK
Sbjct: 938  SRMFSYIYVSTERMCQDVVISTTSILHK 965


>SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 680

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 11/23 (47%), Positives = 15/23 (65%)
 Frame = -3

Query: 748 GPGTLGVHRQTRDREIRSRREPK 680
           G GT  V + TR  + RSRR+P+
Sbjct: 208 GNGTFTVSKTTRKNQPRSRRDPR 230


>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 595

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 14/43 (32%), Positives = 21/43 (48%)
 Frame = -3

Query: 736 LGVHRQTRDREIRSRREPKRQDYDLKELKERQKQQLRHKALKK 608
           L  HR TR     +    K    DLK   E+Q Q+L ++ L++
Sbjct: 494 LSNHRVTRSEMEEATEILKNNSADLKAQLEKQPQELENRLLQE 536


>SPCC16C4.08c |skb15||Shk1 kinase binding protein
           15|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 11/44 (25%), Positives = 23/44 (52%)
 Frame = -2

Query: 728 ASSNSRPRNTISKRAKETGLRLKRAQRKTKAATEAQSSQEGSRP 597
           A SNS P+N   + AK   L  + ++  +++ +E++      +P
Sbjct: 290 ADSNSEPKNVEDEAAKRQSLDSETSETSSESESESEYYSTSKQP 333


>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1254

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = -3

Query: 661 KELKERQKQQLRHKALKKGLDPEALTG 581
           KE+K+RQ+ Q   K +++GL     +G
Sbjct: 461 KEIKDRQRTQKEEKVVQRGLTQVLTSG 487


>SPAC890.05 |||ribosome biogenesis protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 284

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 12/46 (26%), Positives = 25/46 (54%)
 Frame = -2

Query: 722 SNSRPRNTISKRAKETGLRLKRAQRKTKAATEAQSSQEGSRPRSAH 585
           S+S+ R + S   +E   +  + + K ++ +  +  +EG+RP S H
Sbjct: 210 SSSKKRKSGSSDKEEKKKKKIKLKDKPESTSSVEKVKEGNRPASIH 255


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,995,408
Number of Sequences: 5004
Number of extensions: 30930
Number of successful extensions: 145
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)