Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0251
(695 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosoli... 95 4e-20
At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative... 49 3e-06
At1g18310.1 68414.m02288 glycosyl hydrolase family 81 protein si... 30 1.3
At4g33580.1 68417.m04771 carbonic anhydrase family protein / car... 28 5.1
At2g01130.1 68415.m00021 helicase domain-containing protein simi... 28 5.1
At4g16070.1 68417.m02437 lipase class 3 family protein low simil... 28 6.8
At3g32190.1 68416.m04102 hypothetical protein 28 6.8
At3g01500.3 68416.m00076 carbonic anhydrase 1, chloroplast / car... 28 6.8
At3g01500.2 68416.m00075 carbonic anhydrase 1, chloroplast / car... 28 6.8
At3g01500.1 68416.m00074 carbonic anhydrase 1, chloroplast / car... 28 6.8
At4g33970.1 68417.m04820 leucine-rich repeat family protein / ex... 27 9.0
At1g70410.3 68414.m08100 carbonic anhydrase, putative / carbonat... 27 9.0
At1g70410.2 68414.m08101 carbonic anhydrase, putative / carbonat... 27 9.0
At1g70410.1 68414.m08099 carbonic anhydrase, putative / carbonat... 27 9.0
At1g64670.1 68414.m07331 hydrolase, alpha/beta fold family prote... 27 9.0
>At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosolic
(PGIC) identical to SP|P34795 Glucose-6-phosphate
isomerase, cytosolic (EC 5.3.1.9) (GPI) (Phosphoglucose
isomerase) (PGI) (Phosphohexose isomerase) (PHI)
{Arabidopsis thaliana}; contains Pfam profile PF00342:
glucose-6-phosphate isomerase
Length = 560
Score = 95.1 bits (226), Expect = 4e-20
Identities = 49/132 (37%), Positives = 78/132 (59%), Gaps = 2/132 (1%)
Frame = -2
Query: 652 YQLIHQGTRLIP--WISSLQPKLTIQFRMVFTTRSSS*FLGSNRSPDEGQTADEAKAELE 479
YQLIHQG R+IP +I ++ + + + + PD + +L+
Sbjct: 395 YQLIHQG-RVIPCDFIGIVKSQQPVYLKGEVVSNHDELMSNFFAQPD-ALAYGKTPEQLQ 452
Query: 478 KSGMAPEAIDKILPHKVFKGNRPTNSIVLKKITPFTLGALIAMYEHKIFTQGVIWDINSY 299
K ++ + ++PHK F GNRP+ S++L ++T + +G L+A+YEH++ QG +W INS+
Sbjct: 453 KENVS----ENLIPHKTFSGNRPSLSLLLPELTAYNVGQLLAIYEHRVAVQGFVWGINSF 508
Query: 298 DQWGVELGKQLA 263
DQWGVELGK LA
Sbjct: 509 DQWGVELGKVLA 520
Score = 33.1 bits (72), Expect = 0.18
Identities = 26/69 (37%), Positives = 33/69 (47%), Gaps = 4/69 (5%)
Frame = -3
Query: 684 GEPGTNGQHASTSSYTRE---PD*FHGFHRSSPNSQSNFEWCSPQDPL-ANFLAQTEALM 517
GEPGTNGQH+ + P F G +S E S D L +NF AQ +AL
Sbjct: 384 GEPGTNGQHSFYQLIHQGRVIPCDFIGIVKSQQPVYLKGEVVSNHDELMSNFFAQPDALA 443
Query: 516 KGKQLTRLK 490
GK +L+
Sbjct: 444 YGKTPEQLQ 452
>At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative
similar to glucose-6-phosphate isomerase [Spinacia
oleracea] GI:3413511; contains Pfam profile PF00342:
glucose-6-phosphate isomerase
Length = 613
Score = 48.8 bits (111), Expect = 3e-06
Identities = 29/83 (34%), Positives = 50/83 (60%), Gaps = 2/83 (2%)
Frame = -2
Query: 430 VFKGNRPTNSIVLKKITPFTLGALIAMYEHKIFTQGVIWDINSYDQWGVELGKQ-LAKAM 254
++ R + S+ ++++TP ++GA+IA+YE + I +IN+Y Q GVE GK+ A+ +
Sbjct: 474 LYANGRESISVTIQEVTPTSVGAIIALYERAVGLYASIVNINAYHQPGVEAGKKAAAEVL 533
Query: 253 SLSCRGLQ-L*PATTLPRMDSLT 188
+L R L L AT ++ LT
Sbjct: 534 ALQKRVLSVLNEATCKDPVEPLT 556
>At1g18310.1 68414.m02288 glycosyl hydrolase family 81 protein
similar to beta-glucan binding protein GI:6625560 from
[Phaseolus vulgaris]
Length = 649
Score = 30.3 bits (65), Expect = 1.3
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +2
Query: 287 SPLIV*IDIPDHTLREDLVFVHGNKCS*SEWSDLLQNNR 403
S L+V +D P LR V G+ S ++WSD L NN+
Sbjct: 110 SDLVVTLDFPSSNLR--FFLVRGSPSSSTKWSDKLTNNQ 146
>At4g33580.1 68417.m04771 carbonic anhydrase family protein /
carbonate dehydratase family protein similar to
SP|P46512 Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonate
dehydratase 1) {Flaveria linearis}; contains Pfam
profile PF00484: Carbonic anhydrase
Length = 301
Score = 28.3 bits (60), Expect = 5.1
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 450 SIASGAIPDFSSSALASSAVCPSSGLRFEP 539
++A P F A A S VCPS+ L F+P
Sbjct: 106 NLADAQAPKFLVIACADSRVCPSAVLGFQP 135
>At2g01130.1 68415.m00021 helicase domain-containing protein similar
to DEIH-box RNA/DNA helicase [Arabidopsis thaliana]
GI:5881579; contains Pfam profiles PF04408: Helicase
associated domain (HA2), PF00271: Helicase conserved
C-terminal domain
Length = 1112
Score = 28.3 bits (60), Expect = 5.1
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +3
Query: 414 RFPLNTLCGRILSIASGAIPDFSSSALAS 500
R PL++LC +I S+ G+I +F S AL S
Sbjct: 643 RTPLHSLCLQIKSLNLGSISEFLSRALQS 671
>At4g16070.1 68417.m02437 lipase class 3 family protein low
similarity to calmodulin-binding heat-shock protein
CaMBP [Nicotiana tabacum] GI:1087073; contains Pfam
profile PF01764: Lipase, PF03893: Lipase 3 N-terminal
region
Length = 654
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = -2
Query: 367 GALIAMYEHKIFTQGVIWDINSYDQWGVELGKQLAKAMSLSCRGLQL*PATTLPRMDSLT 188
GA++ +Y + ++ ++W N D G ELGK R PAT L + +L+
Sbjct: 11 GAVVILY---LLSRRIVWARNGEDDSGGELGKSGRSGRRRIVRRPAQAPATWLETISTLS 67
Query: 187 SSRR 176
+ R
Sbjct: 68 ETLR 71
>At3g32190.1 68416.m04102 hypothetical protein
Length = 358
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -3
Query: 657 ASTSSYTREPD*FHGFHRSSPN-SQSNFEWCSPQDPL 550
A+ S + P F F SP S+S +W SP DP+
Sbjct: 232 AALPSSSFNPQQFEEFFTESPPLSESGLDWTSPSDPV 268
>At3g01500.3 68416.m00076 carbonic anhydrase 1, chloroplast /
carbonate dehydratase 1 (CA1) nearly identical to
SP|P27140 Carbonic anhydrase, chloroplast precursor (EC
4.2.1.1) (Carbonate dehydratase) {Arabidopsis thaliana}
Length = 336
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +3
Query: 453 IASGAIPDFSSSALASSAVCPSSGLRFEPRN*LEDLVVNTIRNWI 587
+A G P + A + S VCPS L F+P + VV I N +
Sbjct: 154 LAKGQSPKYMVFACSDSRVCPSHVLDFQPG---DAFVVRNIANMV 195
>At3g01500.2 68416.m00075 carbonic anhydrase 1, chloroplast /
carbonate dehydratase 1 (CA1) nearly identical to
SP|P27140 Carbonic anhydrase, chloroplast precursor (EC
4.2.1.1) (Carbonate dehydratase) {Arabidopsis thaliana}
Length = 347
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +3
Query: 453 IASGAIPDFSSSALASSAVCPSSGLRFEPRN*LEDLVVNTIRNWI 587
+A G P + A + S VCPS L F+P + VV I N +
Sbjct: 154 LAKGQSPKYMVFACSDSRVCPSHVLDFQPG---DAFVVRNIANMV 195
>At3g01500.1 68416.m00074 carbonic anhydrase 1, chloroplast /
carbonate dehydratase 1 (CA1) nearly identical to
SP|P27140 Carbonic anhydrase, chloroplast precursor (EC
4.2.1.1) (Carbonate dehydratase) {Arabidopsis thaliana}
Length = 270
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +3
Query: 453 IASGAIPDFSSSALASSAVCPSSGLRFEPRN*LEDLVVNTIRNWI 587
+A G P + A + S VCPS L F+P + VV I N +
Sbjct: 77 LAKGQSPKYMVFACSDSRVCPSHVLDFQPG---DAFVVRNIANMV 118
>At4g33970.1 68417.m04820 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 699
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -1
Query: 695 PIVWASPGPTDSTPLPAHTPGNQIDSMDFIAPAQTHNP 582
P V++ P P S P P H+P + S PA H+P
Sbjct: 570 PPVFSPPPPVYSPPPPVHSPPPPVHSPP--PPAPVHSP 605
>At1g70410.3 68414.m08100 carbonic anhydrase, putative / carbonate
dehydratase, putative similar to SP|P42737 Carbonic
anhydrase 2 (EC 4.2.1.1) (Carbonate dehydratase 2)
{Arabidopsis thaliana}; contains Pfam profile PF00484:
Carbonic anhydrase
Length = 258
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/45 (37%), Positives = 21/45 (46%)
Frame = +3
Query: 453 IASGAIPDFSSSALASSAVCPSSGLRFEPRN*LEDLVVNTIRNWI 587
+A P F A + S VCPS L F+P E VV I N +
Sbjct: 74 LAKTQTPKFLVFACSDSRVCPSHILNFQPG---EAFVVRNIANMV 115
>At1g70410.2 68414.m08101 carbonic anhydrase, putative / carbonate
dehydratase, putative similar to SP|P42737 Carbonic
anhydrase 2 (EC 4.2.1.1) (Carbonate dehydratase 2)
{Arabidopsis thaliana}; contains Pfam profile PF00484:
Carbonic anhydrase
Length = 280
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/45 (37%), Positives = 21/45 (46%)
Frame = +3
Query: 453 IASGAIPDFSSSALASSAVCPSSGLRFEPRN*LEDLVVNTIRNWI 587
+A P F A + S VCPS L F+P E VV I N +
Sbjct: 96 LAKTQTPKFLVFACSDSRVCPSHILNFQPG---EAFVVRNIANMV 137
>At1g70410.1 68414.m08099 carbonic anhydrase, putative / carbonate
dehydratase, putative similar to SP|P42737 Carbonic
anhydrase 2 (EC 4.2.1.1) (Carbonate dehydratase 2)
{Arabidopsis thaliana}; contains Pfam profile PF00484:
Carbonic anhydrase
Length = 258
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/45 (37%), Positives = 21/45 (46%)
Frame = +3
Query: 453 IASGAIPDFSSSALASSAVCPSSGLRFEPRN*LEDLVVNTIRNWI 587
+A P F A + S VCPS L F+P E VV I N +
Sbjct: 74 LAKTQTPKFLVFACSDSRVCPSHILNFQPG---EAFVVRNIANMV 115
>At1g64670.1 68414.m07331 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas putida] GI:2822275; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 469
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +2
Query: 272 LAEFNSPLIV*IDIP-DHTLREDLVFVHGNKCS*SEWSDLLQNN 400
L+ N L V + P D+ +E++VF+HG S + W++ L N
Sbjct: 162 LSSSNQSLFVNVQQPTDNKAQENVVFIHGFLSSSTFWTETLFPN 205
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,004,801
Number of Sequences: 28952
Number of extensions: 345594
Number of successful extensions: 897
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 889
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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