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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVm0190
         (741 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)             110   1e-24
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)                     105   3e-23
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)              54   2e-07
SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12)                 31   0.98 
SB_10602| Best HMM Match : RVT_1 (HMM E-Value=1.3e-10)                 29   4.0  
SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012)                 28   9.1  

>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 309

 Score =  110 bits (264), Expect = 1e-24
 Identities = 53/82 (64%), Positives = 65/82 (79%), Gaps = 1/82 (1%)
 Frame = -2

Query: 560 GENAAHTPHKAGSGVKFPVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLI 381
           G+ A   P + G  + F VF+YEI  + ++AC+LAKQAFDDAIAELD+LNED YKDSTLI
Sbjct: 228 GKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAFDDAIAELDSLNEDQYKDSTLI 287

Query: 380 MQLLRDNLTLWTSDTQGD-GDE 318
           MQLLRDNLTLW+S+ Q D GD+
Sbjct: 288 MQLLRDNLTLWSSENQEDQGDD 309



 Score = 98.3 bits (234), Expect = 6e-21
 Identities = 50/82 (60%), Positives = 58/82 (70%), Gaps = 2/82 (2%)
 Frame = -2

Query: 539 PHKAGSGVKFPVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDN 360
           P + G  + F VFYYEI+    +AC LAK+AFDDAIAELDTL+ED YKDSTLIMQLLRDN
Sbjct: 146 PIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELDTLSEDQYKDSTLIMQLLRDN 205

Query: 359 LTLWTSDTQG--DGDEPAEGGD 300
           LT+     Q   +  E AE GD
Sbjct: 206 LTVVEKALQAYKEAKEAAETGD 227



 Score = 96.3 bits (229), Expect = 2e-20
 Identities = 49/82 (59%), Positives = 58/82 (70%), Gaps = 2/82 (2%)
 Frame = -3

Query: 739 LGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISK 560
           L LL+  LIP A + ESKVFYLKMKGDYYRY  EVA  + R  VV+ + KAY +A EI++
Sbjct: 77  LKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVAGADRRREVVQKAMKAYSEAQEIAE 136

Query: 559 --AKMQPTHPIRLGLALNFQSF 500
              K+ PT PIRLGLALNF  F
Sbjct: 137 KDPKLPPTDPIRLGLALNFSVF 158



 Score = 40.3 bits (90), Expect = 0.002
 Identities = 21/41 (51%), Positives = 29/41 (70%), Gaps = 3/41 (7%)
 Frame = -3

Query: 613 SVVEDSQKAYQDAFEISKA---KMQPTHPIRLGLALNFQSF 500
           +VVE + +AY++A E ++    K+ PT PIRLGLALNF  F
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVF 247


>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
          Length = 248

 Score =  105 bits (253), Expect = 3e-23
 Identities = 48/74 (64%), Positives = 58/74 (78%)
 Frame = -2

Query: 539 PHKAGSGVKFPVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDN 360
           P + G  + F VFYYEI N P +AC+LAK+AFDDAIA LD L ++SYKDSTLIMQLLRDN
Sbjct: 173 PIRLGLALNFSVFYYEIENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIMQLLRDN 232

Query: 359 LTLWTSDTQGDGDE 318
           LTLWTS+   +G +
Sbjct: 233 LTLWTSEQDQEGQD 246



 Score = 86.2 bits (204), Expect = 2e-17
 Identities = 42/83 (50%), Positives = 58/83 (69%), Gaps = 3/83 (3%)
 Frame = -3

Query: 739 LGLLDKHLIPKAS---NPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFE 569
           L +L+ +L+       N E+KVFYLKM+GDY+RYL EVA G++R   +E S++AY+DA  
Sbjct: 104 LDILENYLLKDGQDDINTEAKVFYLKMRGDYHRYLVEVAEGDSRKENIEKSREAYKDA-S 162

Query: 568 ISKAKMQPTHPIRLGLALNFQSF 500
               ++ P+HPIRLGLALNF  F
Sbjct: 163 AKAEELSPSHPIRLGLALNFSVF 185


>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 219

 Score = 53.6 bits (123), Expect = 2e-07
 Identities = 24/39 (61%), Positives = 32/39 (82%)
 Frame = -3

Query: 739 LGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGE 623
           LG+L++ LIP A + E+KVFY K+KGDYYRYLAE + G+
Sbjct: 178 LGILER-LIPGAEDEENKVFYFKLKGDYYRYLAEFSHGQ 215


>SB_23213| Best HMM Match : SH3_1 (HMM E-Value=9.2e-12)
          Length = 979

 Score = 31.1 bits (67), Expect = 0.98
 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 3/49 (6%)
 Frame = -2

Query: 443 DDAIAELDTLNEDSYKDSTLIMQL---LRDNLTLWTSDTQGDGDEPAEG 306
           DD   + D  ++DS  + +   QL   L+  + LW S TQGD D  A G
Sbjct: 850 DDDDFDTDEWSDDSDAEGSAAGQLKLCLKREILLWKSGTQGDADRRATG 898


>SB_10602| Best HMM Match : RVT_1 (HMM E-Value=1.3e-10)
          Length = 416

 Score = 29.1 bits (62), Expect = 4.0
 Identities = 16/61 (26%), Positives = 30/61 (49%)
 Frame = -3

Query: 739 LGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISK 560
           LGL++ H+ PK+ +   ++F      +Y+ +L  V    T+   +E +   Y    + SK
Sbjct: 117 LGLIESHIDPKSFH--HRMFLAACCLEYFGFLRSVEFTVTQEKCIEQNMPLYAVFIDFSK 174

Query: 559 A 557
           A
Sbjct: 175 A 175


>SB_3346| Best HMM Match : Spectrin (HMM E-Value=0.012)
          Length = 983

 Score = 27.9 bits (59), Expect = 9.1
 Identities = 17/58 (29%), Positives = 27/58 (46%)
 Frame = -2

Query: 500 YYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQGD 327
           YY I  +  K      Q FDD    +   +ED+ +D+T  ++L+       T+D  GD
Sbjct: 505 YYNIPYNKPKKNDFKLQLFDDYKQAVTQASEDTLRDATTAIELMNAP----TTDVHGD 558


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,823,310
Number of Sequences: 59808
Number of extensions: 447752
Number of successful extensions: 1392
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1291
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1388
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1998111622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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