Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0185
(623 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 78 1e-15
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 34 0.019
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 31 0.14
SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.41
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 28 0.95
SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces p... 28 1.3
SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase Snf22... 27 2.9
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 26 3.8
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 26 3.8
SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 25 6.7
SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 25 6.7
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S... 25 6.7
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 25 8.9
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 8.9
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 77.8 bits (183), Expect = 1e-15
Identities = 37/70 (52%), Positives = 47/70 (67%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLKREMAVYGCPPVFP 425
L GH F+LSK EF + RL G+ VLFP GFHCTGMPI A AD+L RE+ ++G P
Sbjct: 80 LHLGHAFTLSKVEFTTAFERLNGKRVLFPMGFHCTGMPICASADRLSREIEMFGPSFDVP 139
Query: 426 EDEELIVKDE 455
E++E V+ E
Sbjct: 140 EEKEEEVEVE 149
Score = 57.6 bits (133), Expect = 1e-09
Identities = 30/65 (46%), Positives = 40/65 (61%), Gaps = 7/65 (10%)
Frame = +1
Query: 82 LDRKGTFKVEYLQEIEKKVQERWESQKIFEVEAP-DD------GKSHEKFLCTFPYPYMN 240
L + T K + L E+EKK Q++W+ +K FEV+AP +D K + KF PYPYMN
Sbjct: 18 LKLENTTKRDTLIELEKKYQQKWQEEKAFEVDAPLEDVPIDELRKKYPKFFGNMPYPYMN 77
Query: 241 GRLHL 255
G LHL
Sbjct: 78 GALHL 82
Score = 53.6 bits (123), Expect = 2e-08
Identities = 21/32 (65%), Positives = 26/32 (81%)
Frame = +2
Query: 521 KYQWQIMKSIGVPEEEIKEFANESYWLEYFPP 616
KYQ+QIM+S+GVP EI +FA+ YWL YFPP
Sbjct: 177 KYQFQIMESLGVPRTEIHKFADAKYWLSYFPP 208
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 33.9 bits (74), Expect = 0.019
Identities = 15/43 (34%), Positives = 22/43 (51%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACA 374
L GH + + RYYR+KG V+ P G+ G+P + A
Sbjct: 73 LHIGHVRVYTISDILSRYYRMKGYKVIHPMGWDAFGLPAENAA 115
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 31.1 bits (67), Expect = 0.14
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLKRE 392
L GH + + R+ LKGR V + G+ C G+PI++ A K E
Sbjct: 93 LHIGHALNKILKDIINRWQLLKGRSVHYVPGWDCHGLPIESKAIKANAE 141
>SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 489
Score = 29.5 bits (63), Expect = 0.41
Identities = 18/37 (48%), Positives = 22/37 (59%)
Frame = -1
Query: 200 CDFPSSGASTSKIFWLSHLS*TFFSIS*RYSTLNVPF 90
C P+S AS S I W SHL+ +FS+ R S NV F
Sbjct: 246 CLLPASFASQSTIAWQSHLNSYYFSLRTR-SLNNVLF 281
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 28.3 bits (60), Expect = 0.95
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADK 380
L GH +++ + R+ R+ G+ VLF GF G+ ++ +K
Sbjct: 145 LHIGHALTIAIQDSLARWNRMLGKTVLFLGGFDHAGLSTQSVVEK 189
>SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 832
Score = 27.9 bits (59), Expect = 1.3
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 7/49 (14%)
Frame = +3
Query: 294 RYYRLKGRMVLFPFG-------FHCTGMPIKACADKLKREMAVYGCPPV 419
+YY R VLFPFG F + + +K C ++L+ ++ + P+
Sbjct: 689 KYYEAVEREVLFPFGYGLSYTTFELSNLYLKNCGERLRIDLEISNTGPM 737
>SPCC1620.14c |snf22|SPCC830.01c|ATP-dependent DNA helicase
Snf22|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1680
Score = 26.6 bits (56), Expect = 2.9
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 527 QWQIMKSIGVPEEEIKEFANES 592
QWQ+M++ G EE EFA S
Sbjct: 25 QWQVMRNQGATEENNPEFAQIS 46
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 26.2 bits (55), Expect = 3.8
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -1
Query: 380 LISTSFNRHPSTVESKWKQHHSTFQP 303
L S S +RH + SK KQH QP
Sbjct: 695 LASLSASRHADAISSKSKQHTEIAQP 720
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 26.2 bits (55), Expect = 3.8
Identities = 13/53 (24%), Positives = 25/53 (47%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLKREMAVY 404
L GH +++ + R+Y + G V F G G+ ++ +K ++ VY
Sbjct: 73 LHIGHALTITIQDALARFYAMHGYKVSFRPGTDHAGIATQSVVEKYLQKKGVY 125
>SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 425
Score = 25.4 bits (53), Expect = 6.7
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = -1
Query: 356 HPSTVESKWKQHHSTFQPVVPAGKLT 279
H S +WKQH + + P GK T
Sbjct: 233 HQSVNLKRWKQHPDQIEFIPPDGKFT 258
>SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1150
Score = 25.4 bits (53), Expect = 6.7
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = -3
Query: 336 QMETAPFYLSTGSTCRQTHIWIEKMYDQMQASIHVWIWKSTQKLLM 199
+++ PFY S T I D+MQ++ +IWK QK+L+
Sbjct: 999 RLKNLPFYFSLHQT-----IGPLPFKDEMQSTTIEYIWKIPQKVLL 1039
>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
Sad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1
Query: 103 KVEYLQEIEKKVQERWESQKIFEVEAPDDGK 195
KVE L++ K+ ++S FEVE DD K
Sbjct: 266 KVEQLKQSLKEEMSNYKSSVPFEVELNDDWK 296
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 25.0 bits (52), Expect = 8.9
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +3
Query: 255 GHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADK 380
GH + + + RY LKG V FG+ G+P++ DK
Sbjct: 51 GHLLASTIKDSVTRYACLKGYHVERRFGWDTHGLPVEHEIDK 92
>SPAC1093.03 |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 832
Score = 25.0 bits (52), Expect = 8.9
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = -1
Query: 371 TSFNRHPSTVESKWKQHHSTFQPVVPAG 288
TS+ +H ++ +W Q S P P G
Sbjct: 283 TSYVQHRGSIPLRWSQEFSNITPKPPIG 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,641,874
Number of Sequences: 5004
Number of extensions: 56156
Number of successful extensions: 162
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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