Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0185
(623 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)... 91 4e-19
At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative / leuc... 31 0.47
At2g17110.1 68415.m01974 expressed protein 31 0.47
At4g19990.1 68417.m02927 far-red impaired responsive family prot... 31 0.82
At3g45510.1 68416.m04914 zinc finger (C3HC4-type RING finger) fa... 31 0.82
At1g17360.1 68414.m02116 COP1-interacting protein-related simila... 29 1.9
At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12) ... 29 2.5
At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative / i... 29 3.3
At2g26130.1 68415.m03135 zinc finger (C3HC4-type RING finger) fa... 28 4.4
At5g01860.1 68418.m00105 zinc finger (C2H2 type) family protein ... 28 5.8
At4g35240.1 68417.m05009 expressed protein contains Pfam domains... 28 5.8
At3g25130.1 68416.m03138 expressed protein 28 5.8
At2g32490.1 68415.m03970 3'-5' exonuclease domain-containing pro... 28 5.8
At1g20850.1 68414.m02612 cysteine endopeptidase, papain-type (XC... 28 5.8
>At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)
family protein similar to cytosolic leucyl-tRNA
synthetase [Candida albicans] GI:9858190; contains Pfam
profile PF00133: tRNA synthetases class I (I, L, M and
V)
Length = 1084
Score = 91.5 bits (217), Expect = 4e-19
Identities = 40/63 (63%), Positives = 48/63 (76%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADKLKREMAVYGCPPVFP 425
L GH FSLSK +FA Y+RL+G VL PFGFHCTGMPIKA ADKL+RE+ +G PPVF
Sbjct: 59 LHIGHAFSLSKVDFASAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEQFGNPPVFT 118
Query: 426 EDE 434
++
Sbjct: 119 AED 121
Score = 54.4 bits (125), Expect = 6e-08
Identities = 26/61 (42%), Positives = 38/61 (62%), Gaps = 1/61 (1%)
Frame = +1
Query: 76 MALDRKGTFKVEYLQEIEKKVQERWESQKIFEVEAPDD-GKSHEKFLCTFPYPYMNGRLH 252
MA + K + + L EIE V++ WE + +F E+ ++ K EKF TFP+PYMNG LH
Sbjct: 1 MASESKSYARRDRLLEIEATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYLH 60
Query: 253 L 255
+
Sbjct: 61 I 61
Score = 50.0 bits (114), Expect = 1e-06
Identities = 20/37 (54%), Positives = 24/37 (64%)
Frame = +2
Query: 512 GGSKYQWQIMKSIGVPEEEIKEFANESYWLEYFPPRA 622
GG YQW+IM+S G+ + EI F S WL YFPP A
Sbjct: 156 GGQVYQWEIMRSFGLTDSEIANFREPSEWLYYFPPLA 192
>At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative /
leucine--tRNA ligase, putative similar to SP|P36430
Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine--tRNA
ligase) (LeuRS) {Bacillus subtilis}; contains Pfam
profile PF00133: tRNA synthetases class I (I, L, M and
V)
Length = 973
Score = 31.5 bits (68), Expect = 0.47
Identities = 24/54 (44%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
Frame = +1
Query: 103 KVEYLQEIEKKVQERWESQKIFEVEAPDD-GKSHEKF--LCTFPYPYMNGRLHL 255
+V EIE K Q WE +IF PDD S KF L FPYP G LH+
Sbjct: 83 RVYPFHEIEPKWQRYWEDNRIF--RTPDDVDTSKPKFYVLDMFPYPSGAG-LHV 133
Score = 27.9 bits (59), Expect = 5.8
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = +3
Query: 246 LAFGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACA 374
L GH + + R R++G VL P G+ G+P + A
Sbjct: 131 LHVGHPLGYTATDILARLRRMQGYNVLHPMGWDAFGLPAEQYA 173
>At2g17110.1 68415.m01974 expressed protein
Length = 733
Score = 31.5 bits (68), Expect = 0.47
Identities = 13/39 (33%), Positives = 24/39 (61%)
Frame = -3
Query: 360 SASQYSGIQMETAPFYLSTGSTCRQTHIWIEKMYDQMQA 244
+A Y+ I+ E A + ST + H+W +K+YD+++A
Sbjct: 383 TAPTYADIEAELALKSRNLSSTLHKLHLWEKKLYDEVKA 421
>At4g19990.1 68417.m02927 far-red impaired responsive family protein
/ FAR1 family protein similar to far-red impaired
response protein [Arabidopsis thaliana] GI:5764395;
contains Pfam profile PF03101: FAR1 family
Length = 672
Score = 30.7 bits (66), Expect = 0.82
Identities = 13/32 (40%), Positives = 23/32 (71%)
Frame = +1
Query: 82 LDRKGTFKVEYLQEIEKKVQERWESQKIFEVE 177
+ RK TFK +L++ +K +QER+E ++ E+E
Sbjct: 392 IQRKTTFKA-FLEQYKKMIQERYEEEEKSEIE 422
>At3g45510.1 68416.m04914 zinc finger (C3HC4-type RING finger)
family protein contains a zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 257
Score = 30.7 bits (66), Expect = 0.82
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -1
Query: 275 G*RKCMTKCKRPFMYGYG-KVHKNFSC-DFPSSGASTSKIFWL 153
G R+C +KC++PF H N SC ++ G + WL
Sbjct: 174 GVRRCCSKCRKPFCIDCNVSWHSNLSCKEYKMKGNKATTTVWL 216
>At1g17360.1 68414.m02116 COP1-interacting protein-related similar
to COP1-Interacting Protein 7 (CIP7) (GI:3327870)
[Arabidopsis thaliana]
Length = 1032
Score = 29.5 bits (63), Expect = 1.9
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +1
Query: 58 NIYSLKMALDRKGTFKVEYLQEIEKKVQERWESQK 162
N+ L+ + D KG EY+++ + K++E W S++
Sbjct: 541 NLSELRFSDDSKGKLYEEYMKKRDAKLREEWSSKE 575
>At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12)
almost identical to ubiquitin-specific protease 12
GI:11993471 [Arabidopsis thaliana], one amino acid
difference
Length = 1115
Score = 29.1 bits (62), Expect = 2.5
Identities = 10/18 (55%), Positives = 15/18 (83%)
Frame = +3
Query: 411 PPVFPEDEELIVKDEDII 464
PPV PEDEE++V + D++
Sbjct: 7 PPVDPEDEEMLVPNSDLV 24
>At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative /
isoleucine--tRNA ligase, putative similar to SP|P41252
Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5)
(Isoleucine--tRNA ligase) (IleRS) (IRS) {Homo sapiens};
contains Pfam profile PF00133: tRNA synthetases class I
(I, L, M and V)
Length = 1190
Score = 28.7 bits (61), Expect = 3.3
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = +3
Query: 252 FGHTFSLSKCEFACRYYRLKGRMVLFPFGFHCTGMPIKACADK 380
+GH + + + RY + G V FG+ C G+P++ D+
Sbjct: 57 YGHILAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDR 99
>At2g26130.1 68415.m03135 zinc finger (C3HC4-type RING finger)
family protein contains a zinc finger, C3HC4 type (RING
finger), signature, PROSITE:PS00518
Length = 398
Score = 28.3 bits (60), Expect = 4.4
Identities = 17/43 (39%), Positives = 18/43 (41%), Gaps = 2/43 (4%)
Frame = -1
Query: 317 STFQPVVPAGKLTFG*RKCMTKCKRPFMYGYGKV--HKNFSCD 195
ST L G R+C KC PF KV H N SCD
Sbjct: 252 STLMSETELSGLNIGVRRCCVKCGEPFCVKC-KVSWHNNLSCD 293
>At5g01860.1 68418.m00105 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 215
Score = 27.9 bits (59), Expect = 5.8
Identities = 18/61 (29%), Positives = 26/61 (42%)
Frame = -2
Query: 250 ASVHSCMDMEKYTKTSHVISHHQELQLQKSSGSPIFPELSSQFPEDIQP*MYPSCPMPFS 71
A V +C +K TS + HQ Q+ S + E+ +PE YP + FS
Sbjct: 64 ARVFACTFCKKEFSTSQALGGHQNAHKQERSLAKRRKEIEINYPELSIYSQYPPSGLSFS 123
Query: 70 S 68
S
Sbjct: 124 S 124
>At4g35240.1 68417.m05009 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 828
Score = 27.9 bits (59), Expect = 5.8
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = -3
Query: 348 YSGIQMETAPFYLSTGSTCRQTHIWIEKMYDQMQA 244
Y+ I+ E A + ST + H+W +K+Y +++A
Sbjct: 472 YADIEEELASRSRNLSSTLHKLHLWEKKLYHEVKA 506
>At3g25130.1 68416.m03138 expressed protein
Length = 406
Score = 27.9 bits (59), Expect = 5.8
Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 8/63 (12%)
Frame = +1
Query: 97 TFKVEYLQEIEKKVQERWES--------QKIFEVEAPDDGKSHEKFLCTFPYPYMNGRLH 252
T K + +E +KK++++ +S +++ E DDG H++ C + G++H
Sbjct: 316 TEKKQQTEEEKKKLKKKTKSMMKTKSIEKEVIVEEEDDDGIDHQQLCCLQALKFSTGKMH 375
Query: 253 LVI 261
L I
Sbjct: 376 LGI 378
>At2g32490.1 68415.m03970 3'-5' exonuclease domain-containing
protein contains Pfam profile PF01612: 3'-5' exonuclease
Length = 217
Score = 27.9 bits (59), Expect = 5.8
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 394 ISRFSLSAQALIGIPVQWNPNGN 326
+SR LS ++G+ VQW P G+
Sbjct: 56 VSRLRLSHPLVVGLGVQWTPRGS 78
>At1g20850.1 68414.m02612 cysteine endopeptidase, papain-type (XCP2)
identical to papain-type cysteine endopeptidase XCP2
GI:6708183 from [Arabidopsis thaliana]
Length = 356
Score = 27.9 bits (59), Expect = 5.8
Identities = 17/69 (24%), Positives = 31/69 (44%)
Frame = -2
Query: 382 SLSAQALIGIPVQWNPNGNSTILPFNR*YLQANSHLDRENV*PNASVHSCMDMEKYTKTS 203
+LS Q LI +N N ++ + Y+ N L +E P + +M+K +
Sbjct: 184 TLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKDESET 243
Query: 202 HVISHHQEL 176
I+ HQ++
Sbjct: 244 VTINGHQDV 252
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,513,584
Number of Sequences: 28952
Number of extensions: 283161
Number of successful extensions: 783
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 782
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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