BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0108 (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 105 6e-24 SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 62 9e-11 SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 53 3e-08 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 38 0.001 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 38 0.002 SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 29 0.80 SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 27 2.4 SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 25 7.5 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 25 7.5 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 7.5 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 25 7.5 SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 25 9.9 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 9.9 >SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 360 Score = 105 bits (252), Expect = 6e-24 Identities = 44/84 (52%), Positives = 57/84 (67%) Frame = +2 Query: 257 GVPCRYCEFCKTGRYHLCPDMIFCATPPVHGNLVRYYKHAADFCFKLPDHVTMEEGALLE 436 G CR C++C++GRY+LCP M F ATPP G L YY DFC KLP +++EEGAL E Sbjct: 94 GCVCRLCDYCRSGRYNLCPHMEFAATPPYDGTLRTYYITTEDFCTKLPKQISVEEGALFE 153 Query: 437 PLAVGIHACKRGGVSAGHVVLVLG 508 P++V +HA RG + G VLV+G Sbjct: 154 PMSVAVHAMTRGNLKCGSRVLVMG 177 Score = 89.4 bits (212), Expect = 4e-19 Identities = 39/87 (44%), Positives = 59/87 (67%), Gaps = 1/87 (1%) Frame = +3 Query: 6 LLYKPNDLRLVQTPIPEISED-EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHE 182 +L K D + P +++D +V + + GICGSDVHYW++G G F+L++PMI+GHE Sbjct: 9 VLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHE 68 Query: 183 ASGVVAKIGSKVKNLTVGDRVAIEPAC 263 ++GVV ++G V +L GD VA+EP C Sbjct: 69 SAGVVVEVGKGVSSLKPGDPVAVEPGC 95 Score = 27.9 bits (59), Expect = 1.4 Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = +1 Query: 541 AKAFGAHKVLIIDILQSRLDFA-KSLGADYTLLIGKDSNEA 660 AKA+GA ++ +D SR++FA K +GA I NE+ Sbjct: 189 AKAYGAIDIVAVDASPSRVEFAQKYVGAKPFTPIAAKENES 229 >SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharomyces pombe|chr 3|||Manual Length = 350 Score = 61.7 bits (143), Expect = 9e-11 Identities = 30/81 (37%), Positives = 50/81 (61%), Gaps = 2/81 (2%) Frame = +3 Query: 18 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEASG 191 P +++ + P+ E +DEVL+ + G+C +D+H Q G + L + P+I GHE +G Sbjct: 18 PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGAG 73 Query: 192 VVAKIGSKVKNLTVGDRVAIE 254 VV K+G+ V L +GDRV ++ Sbjct: 74 VVVKVGAGVTRLKIGDRVGVK 94 Score = 25.8 bits (54), Expect = 5.6 Identities = 20/82 (24%), Positives = 31/82 (37%), Gaps = 1/82 (1%) Frame = +2 Query: 266 CRYCEFCKTGRYHLCPDMIFCATPPVHGNLVRYYKHAADFCFKLPDHVTMEEGALLEPLA 445 C CE+C +CP I + V G Y A +P+ V +E A + Sbjct: 100 CGNCEYCMKAEETICPH-IQLSGYTVDGTFQHYCIANATHATIIPESVPLEVAAPIMCAG 158 Query: 446 VGIH-ACKRGGVSAGHVVLVLG 508 + + A K V G + + G Sbjct: 159 ITCYRALKESKVGPGEWICIPG 180 >SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 423 Score = 53.2 bits (122), Expect = 3e-08 Identities = 29/80 (36%), Positives = 48/80 (60%), Gaps = 2/80 (2%) Frame = +3 Query: 18 PNDLRLVQTPIPEISED-EVLLRMDCVGIC-GSDVHYWQKGQCGHFVLEEPMIMGHEASG 191 P ++++ + P P I+ +V+++ IC GSD H + G +E+ I+GHE+ G Sbjct: 45 PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCG 101 Query: 192 VVAKIGSKVKNLTVGDRVAI 251 +VA+ G +V NL +GDRV I Sbjct: 102 IVAEKGDEVNNLEIGDRVVI 121 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 37.9 bits (84), Expect = 0.001 Identities = 26/85 (30%), Positives = 43/85 (50%), Gaps = 2/85 (2%) Frame = +3 Query: 18 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVV 197 P + VQ P + E V +++ G+C +D Y G+ + P+I+GHE +G+V Sbjct: 24 PLSIENVQVFPPRVHE--VRIKIVNSGVCHTDA-YTLSGKDPEGLF--PVILGHEGAGIV 78 Query: 198 AKIGSKVKNLTVGDRV--AIEPACR 266 +G +V + VGD V P C+ Sbjct: 79 ESVGPQVTTVQVGDPVIALYTPECK 103 Score = 28.3 bits (60), Expect = 1.1 Identities = 8/15 (53%), Positives = 13/15 (86%) Frame = +2 Query: 266 CRYCEFCKTGRYHLC 310 C+ C+FCK+G+ +LC Sbjct: 102 CKTCKFCKSGKTNLC 116 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 37.5 bits (83), Expect = 0.002 Identities = 22/68 (32%), Positives = 35/68 (51%), Gaps = 2/68 (2%) Frame = +3 Query: 69 EVLLRMDCVGICGSDVHYWQKGQCGHFVLEEPMIMGHEASGVVAKIGSKVKNLTVGDRVA 248 EV +++D +C +D Y G P+++GHE +G+V IG V N+ GD V Sbjct: 36 EVRVKVDWSAVCHTDA-YTLSGVDPEGAF--PIVLGHEGAGIVESIGEGVINVRPGDHVI 92 Query: 249 I--EPACR 266 + P C+ Sbjct: 93 LLYTPECK 100 Score = 27.1 bits (57), Expect = 2.4 Identities = 7/15 (46%), Positives = 13/15 (86%) Frame = +2 Query: 266 CRYCEFCKTGRYHLC 310 C+ C+FC++G+ +LC Sbjct: 99 CKECKFCRSGKTNLC 113 >SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 346 Score = 28.7 bits (61), Expect = 0.80 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = +3 Query: 153 LEEPMIMGHEASGVVAKIGSKVKNLTVGDRV 245 L+ P++ G + +G++ K+G V+ GD V Sbjct: 58 LQLPVVPGSDGAGIIEKVGEDVEGFEKGDSV 88 >SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 719 Score = 27.1 bits (57), Expect = 2.4 Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 2/33 (6%) Frame = +3 Query: 213 KVKNLTVGDRVAIEPACRVG--TASSVRQDDTT 305 +V + + RVA E CR+G S+R DDTT Sbjct: 151 RVAAVNLAKRVAAEQGCRLGEQVGYSIRFDDTT 183 >SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 906 Score = 25.4 bits (53), Expect = 7.5 Identities = 6/31 (19%), Positives = 13/31 (41%) Frame = +3 Query: 414 WKKERYWSPSRWAFTRARGAESRRDTWCWYW 506 W +++ W + + D WCW++ Sbjct: 821 WLEKKADKARSWFLKAVKADQDNGDVWCWFY 851 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 25.4 bits (53), Expect = 7.5 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 2/37 (5%) Frame = +3 Query: 213 KVKNLTVGDRVAIEPACRVG--TASSVRQDDTTYALT 317 +V ++V RVA E CRVG ++R +D T +T Sbjct: 566 RVAAMSVAKRVAEEVGCRVGEEVGYTIRFEDKTSRMT 602 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 25.4 bits (53), Expect = 7.5 Identities = 11/31 (35%), Positives = 17/31 (54%) Frame = -1 Query: 412 GHVIRKLEAKISCVLVISDEVSVYWGRGAED 320 GH ++ +EAK + I + SV G+G D Sbjct: 211 GHTLKDMEAKSGAKIAIRGKGSVKEGKGRSD 241 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 25.4 bits (53), Expect = 7.5 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = +3 Query: 33 LVQTPIPEISEDEVLLRMDC 92 LVQ+ I E SED + L +DC Sbjct: 1489 LVQSAIRESSEDAIALAVDC 1508 >SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1842 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/41 (34%), Positives = 21/41 (51%) Frame = +3 Query: 237 DRVAIEPACRVGTASSVRQDDTTYALT*SSAPRPQYTETSS 359 D VA EPA + SS + +T A ++ P P +TS+ Sbjct: 95 DEVADEPAEAPASTSSTPKVETAAAAAPAATPAPAPAQTSA 135 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 25.0 bits (52), Expect = 9.9 Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = +3 Query: 195 VAKIGSKVKNLTVGDRVAIEPACRVGTASSVRQDDTTYALT*SSAPRPQYT-ETSSDITS 371 V+ I + VK T + V ++SSV+ +T + SSA PQ T TSS++ S Sbjct: 449 VSSISTSVKQPTAS---VASSSVSVPSSSSVQPQSSTPISSSSSASSPQSTLSTSSEVVS 505 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,896,191 Number of Sequences: 5004 Number of extensions: 59704 Number of successful extensions: 178 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 177 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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