BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0100 (672 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 153 3e-38 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 70 3e-13 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 69 8e-13 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 67 2e-12 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 60 2e-10 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 43 5e-05 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 41 2e-04 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 38 0.001 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.002 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.4 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.5 SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 26 5.7 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 9.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 153 bits (370), Expect = 3e-38 Identities = 68/75 (90%), Positives = 74/75 (98%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKE 328 GGTTMYPGIADRMQKEI ALAPS++K+KI+APPERKYSVWIGGSILASLSTFQQMWISK+ Sbjct: 301 GGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQ 360 Query: 327 EYDESGPGIVHRKCF 283 EYDESGPGIV+RKCF Sbjct: 361 EYDESGPGIVYRKCF 375 Score = 96.3 bits (229), Expect = 4e-21 Identities = 43/51 (84%), Positives = 45/51 (88%) Frame = -2 Query: 671 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYAN 519 QVITIGNERFR PEALFQPS LG+E+ GIHE YNSIMKCDVDIRKDLY N Sbjct: 246 QVITIGNERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGN 296 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 70.1 bits (164), Expect = 3e-13 Identities = 34/80 (42%), Positives = 54/80 (67%), Gaps = 6/80 (7%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASLSTFQQMWI 337 GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGSIL+SL TF Q+WI Sbjct: 354 GGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGSILSSLGTFHQLWI 412 Query: 336 SKEEYDESGP---GIVHRKC 286 S++EY+E G ++ ++C Sbjct: 413 SRQEYEEHGSDRLALIEKRC 432 Score = 26.6 bits (56), Expect = 3.3 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = -2 Query: 590 GIHETVYNSIMKCDVDIRKDLYAN 519 GI + N I +CDVDIR L N Sbjct: 326 GIPQLFQNCISECDVDIRASLLNN 349 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 68.5 bits (160), Expect = 8e-13 Identities = 31/75 (41%), Positives = 51/75 (68%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKE 328 GG+T+ G +R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ E Sbjct: 306 GGSTLLRGFGERFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSE 365 Query: 327 EYDESGPGIVHRKCF 283 EY ++ ++ R+ F Sbjct: 366 EY-KNDQNVIFRRRF 379 Score = 57.2 bits (132), Expect = 2e-09 Identities = 23/51 (45%), Positives = 33/51 (64%) Frame = -2 Query: 671 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYAN 519 Q IT+G E F PE LF P +G E+ G+H ++ SI+ D+D+R LY+N Sbjct: 251 QTITLGTECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSN 301 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 67.3 bits (157), Expect = 2e-12 Identities = 32/68 (47%), Positives = 44/68 (64%), Gaps = 2/68 (2%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITALAP-STIKIKIIAPPER-KYSVWIGGSILASLSTFQQMWIS 334 GGT++ PG+++R+Q E+ LA S I + +VW GGSILASL FQ +W+S Sbjct: 351 GGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVS 410 Query: 333 KEEYDESG 310 K+EYDE G Sbjct: 411 KQEYDEVG 418 Score = 43.6 bits (98), Expect = 3e-05 Identities = 24/55 (43%), Positives = 28/55 (50%), Gaps = 9/55 (16%) Frame = -2 Query: 656 GNERFRCPEALFQPSFLGMESC---------GIHETVYNSIMKCDVDIRKDLYAN 519 G ERFR E LF PSF S G+HE VY SI+ CD ++R L N Sbjct: 292 GQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNN 346 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 60.5 bits (140), Expect = 2e-10 Identities = 25/49 (51%), Positives = 35/49 (71%) Frame = -2 Query: 671 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLY 525 +VI +G+ER+ CPE LFQP +G E G+ E ++++I DVDIRK LY Sbjct: 246 RVIKVGSERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLY 294 Score = 51.6 bits (118), Expect = 1e-07 Identities = 29/78 (37%), Positives = 48/78 (61%), Gaps = 12/78 (15%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITAL--------APSTI---KIKIIAPPERKYSVWIGGSILAS- 364 GG++MY G+ R++KEI L P+ + K+KI P R+++V+IGG++LA Sbjct: 301 GGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADI 360 Query: 363 LSTFQQMWISKEEYDESG 310 ++ MW+SK E++E G Sbjct: 361 MAQNDHMWVSKAEWEEYG 378 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 42.7 bits (96), Expect = 5e-05 Identities = 22/74 (29%), Positives = 37/74 (50%) Frame = -1 Query: 510 VGGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISK 331 +GG +PG R+ E+ +LAP+ ++K+ P + W S + L + I++ Sbjct: 326 IGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITR 384 Query: 330 EEYDESGPGIVHRK 289 EY E G I+ RK Sbjct: 385 SEYSEHGANIMTRK 398 Score = 39.9 bits (89), Expect = 3e-04 Identities = 20/51 (39%), Positives = 27/51 (52%) Frame = -2 Query: 671 QVITIGNERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYAN 519 QV+ + NERF PE LF PS + + GI E V S+ +I+ L N Sbjct: 272 QVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLEN 322 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 40.7 bits (91), Expect = 2e-04 Identities = 19/67 (28%), Positives = 32/67 (47%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITALAPSTIKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKE 328 GG PG+ R+++E+T++ P I + W G S + F+ +++E Sbjct: 642 GGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTRE 701 Query: 327 EYDESGP 307 EY E GP Sbjct: 702 EYLEKGP 708 Score = 33.1 bits (72), Expect = 0.037 Identities = 13/44 (29%), Positives = 27/44 (61%) Frame = -2 Query: 650 ERFRCPEALFQPSFLGMESCGIHETVYNSIMKCDVDIRKDLYAN 519 E+ R PE +F PS +G++ GI E + + + + ++ ++ L +N Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSN 637 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 37.9 bits (84), Expect = 0.001 Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 14/86 (16%) Frame = -1 Query: 507 GGTTMYPGIADRMQKEITALAPSTIK--------------IKIIAPPERKYSVWIGGSIL 370 GG+T++ +R+Q+++ + I + +I+ ++ +VW GGS+L Sbjct: 334 GGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLL 393 Query: 369 ASLSTFQQMWISKEEYDESGPGIVHR 292 A F +K +Y+E G I R Sbjct: 394 AQTPEFGSYCHTKADYEEYGASIARR 419 Score = 35.5 bits (78), Expect = 0.007 Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Frame = -2 Query: 665 ITIGNERFRCPEALFQPSFLGME-SCGIHETVYNSIMKCDVDIRKDLYAN 519 I +G ERF PE F P + + E V N + +D+RK LY N Sbjct: 280 IDVGFERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKN 329 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 37.5 bits (83), Expect = 0.002 Identities = 18/77 (23%), Positives = 35/77 (45%), Gaps = 3/77 (3%) Frame = -1 Query: 510 VGGTTMYPGIADRMQKEITALAPSTIKIKIIAPP---ERKYSVWIGGSILASLSTFQQMW 340 VGG +PG A +++ I + + I +I PP + ++ W G I + ++W Sbjct: 536 VGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYNRIRIVSELW 595 Query: 339 ISKEEYDESGPGIVHRK 289 I ++ G ++ K Sbjct: 596 IKNSDWKMLGSRVLQYK 612 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/37 (35%), Positives = 23/37 (62%), Gaps = 3/37 (8%) Frame = -1 Query: 390 WIGGSILASLSTFQQM---WISKEEYDESGPGIVHRK 289 ++GGSI+A S + + +++ EEY + GP +H K Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -1 Query: 465 KEITALAPSTIKIKIIAPPERKYSVWIGGSILASLST 355 KEI++ A S I + + P E+ W ILA L+T Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248 >SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 981 Score = 25.8 bits (54), Expect = 5.7 Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 2/65 (3%) Frame = -1 Query: 258 GVCGVVLVRVLNATVSTLYLVIP--EN*TSNLTPSILM*FIVKFYINLISFLFLLYTRDR 85 G+ + VR +A S +L P N SN TP+ + I K N+ S + L D Sbjct: 568 GLDSIHSVRFFHAIQSHFHLEGPIRYNMNSNCTPNSIASIIQKKSYNVSSITYELLNEDA 627 Query: 84 CGSQR 70 C R Sbjct: 628 CALSR 632 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -3 Query: 439 DHQDQDHRSPREEVLRMDRWIHPG 368 D D+ R+ E VLRM R++ PG Sbjct: 499 DIDDKKDRALSEHVLRMHRYLPPG 522 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,365,722 Number of Sequences: 5004 Number of extensions: 43309 Number of successful extensions: 127 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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