BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0078 (988 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC15E1.09 |grx2||glutaredoxin Grx2|Schizosaccharomyces pombe|c... 60 6e-10 SPAC4F10.20 |grx1||glutaredoxin Grx1|Schizosaccharomyces pombe|c... 56 1e-08 SPCC1450.06c |grx3||monothiol glutaredoxin Grx3|Schizosaccharomy... 39 0.001 SPAPB2B4.02 |grx5||monothiol glutaredoxin Grx5|Schizosaccharomyc... 30 0.44 >SPAC15E1.09 |grx2||glutaredoxin Grx2|Schizosaccharomyces pombe|chr 1|||Manual Length = 110 Score = 59.7 bits (138), Expect = 6e-10 Identities = 30/79 (37%), Positives = 42/79 (53%) Frame = +1 Query: 10 FXKEAISXXKVVVXXKSXCPXXKLAKDVFEKVKQPIKVIELNERDDGNTXQXNLAQLTGF 189 F ++AIS V V KS CP K AK+ K P K EL++ ++G+ Q L + T Sbjct: 8 FVEKAISNNPVTVFSKSFCPFCKAAKNTLTKYSAPYKAYELDKIENGSDIQAYLHEKTKQ 67 Query: 190 RTVPQVFINGNCVGGGSDV 246 TVP +F +GG SD+ Sbjct: 68 STVPSIFFRNQFIGGNSDL 86 >SPAC4F10.20 |grx1||glutaredoxin Grx1|Schizosaccharomyces pombe|chr 1|||Manual Length = 101 Score = 55.6 bits (128), Expect = 1e-08 Identities = 29/84 (34%), Positives = 45/84 (53%) Frame = +1 Query: 1 IQQFXKEAISXXKVVVXXKSXCPXXKLAKDVFEKVKQPIKVIELNERDDGNTXQXNLAQL 180 ++ F A++ VVV KS CP + V K +V +++ ++G+ Q L + Sbjct: 4 VESFVDSAVADNDVVVFAKSYCPYCHATEKVIADKKIKAQVYQIDLMNNGDEIQSYLLKK 63 Query: 181 TGFRTVPQVFINGNCVGGGSDVKA 252 TG RTVP +FI+ VGG SD +A Sbjct: 64 TGQRTVPNIFIHQKHVGGNSDFQA 87 >SPCC1450.06c |grx3||monothiol glutaredoxin Grx3|Schizosaccharomyces pombe|chr 3|||Manual Length = 166 Score = 38.7 bits (86), Expect = 0.001 Identities = 17/74 (22%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Frame = +1 Query: 40 VVVXXKSXCPXXKLAKDVFE---KVKQPIKVIELNERDDGNTXQXNLAQLTGFRTVPQVF 210 V++ + CP AK + ++ P V+E+ + + + L+ ++ T+P +F Sbjct: 68 VIIFSRPGCPYSAAAKKLLTETLRLDPPAVVVEVTDYEHTQELRDWLSSISDISTMPNIF 127 Query: 211 INGNCVGGGSDVKA 252 + G+ +GG V+A Sbjct: 128 VGGHSIGGSDSVRA 141 >SPAPB2B4.02 |grx5||monothiol glutaredoxin Grx5|Schizosaccharomyces pombe|chr 1|||Manual Length = 146 Score = 30.3 bits (65), Expect = 0.44 Identities = 13/31 (41%), Positives = 22/31 (70%) Frame = +1 Query: 169 LAQLTGFRTVPQVFINGNCVGGGSDVKAXMN 261 + + + + T+PQ++ING V GGSD+ A M+ Sbjct: 86 IKEFSDWPTIPQLYINGEFV-GGSDILASMH 115 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,332,981 Number of Sequences: 5004 Number of extensions: 31885 Number of successful extensions: 50 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 50 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 50 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 509282430 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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