BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVm0045
(630 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 29 0.55
SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharo... 27 3.0
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 3.9
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 26 5.2
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.0
SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting... 25 9.0
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 22 9.8
>SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 372
Score = 29.1 bits (62), Expect = 0.55
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +1
Query: 115 VQTTVKKIHAYSHHLFALRTL 177
++ VKKIH ++H +F LRTL
Sbjct: 63 LKVAVKKIHPFNHPVFCLRTL 83
>SPBC13A2.03 |||phosphatidate
cytidylyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 26.6 bits (56), Expect = 3.0
Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 5/30 (16%)
Frame = +1
Query: 94 RTVLWYVVQTTV-----KKIHAYSHHLFAL 168
R + WY + TT+ + I+AY HHLF +
Sbjct: 109 RFINWYFLMTTLYYAYGESIYAYFHHLFIM 138
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 26.2 bits (55), Expect = 3.9
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
Frame = +1
Query: 58 IFIMLVACVASXRTVLWYVVQTTVKK--IHAYSHHLFAL 168
+F M ACV T LW + T++K IHA S F +
Sbjct: 158 LFYMCRACVIYPITPLWDAKERTMRKATIHALSRIFFLI 196
>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 606
Score = 25.8 bits (54), Expect = 5.2
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +3
Query: 144 IQPPLVCPKNTEHRARHAGKCACCPACVTLLGEG 245
+ PPL PK+ HR AG+ + LL +G
Sbjct: 334 LDPPLD-PKSFSHRCGRAGRAGRAGVAIVLLNDG 366
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 25.0 bits (52), Expect = 9.0
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +1
Query: 61 FIMLVACVASXRTVLWYVV 117
FI+LV C+AS + W V+
Sbjct: 470 FIILVVCIASLYSAFWPVI 488
>SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting
complex subunit Apc4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 719
Score = 25.0 bits (52), Expect = 9.0
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -1
Query: 411 IIFRTGRDRSIASLYFPANENDYTSLVQTLLMHLR 307
+I + G + S+ +LY P +ND S+V M R
Sbjct: 230 LICKGGTNISLKTLYMPLLKNDLGSIVDIATMSTR 264
>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1826
Score = 21.8 bits (44), Expect(2) = 9.8
Identities = 7/17 (41%), Positives = 10/17 (58%)
Frame = -2
Query: 146 YAWIFFTVVCTTYQSTV 96
Y FF++ C YQS +
Sbjct: 540 YVHFFFSIGCVAYQSFI 556
Score = 21.0 bits (42), Expect(2) = 9.8
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = -2
Query: 83 THATSIIKINVFILKFLNTFLVIVLS 6
T ++ + I VFI KF+ ++ + LS
Sbjct: 593 TLLSAALWITVFIAKFVESYYFLTLS 618
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,596,431
Number of Sequences: 5004
Number of extensions: 53041
Number of successful extensions: 136
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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