BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVm0020 (499 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces po... 70 2e-13 SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharo... 48 8e-07 SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pomb... 31 0.096 SPAC922.03 |||1-aminocyclopropane-1-carboxylate deaminase |Schiz... 28 0.90 SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase ... 27 1.2 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 2.1 SPAC23A1.03 |apt1||adenine phosphoribosyltransferase |Schizosacc... 26 3.6 SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 26 3.6 SPAC12G12.15 |sif3||Sad1 interacting factor 3|Schizosaccharomyce... 26 3.6 SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizo... 25 8.4 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 25 8.4 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 8.4 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 8.4 SPAC17A2.14 ||SPAC17G6.01|CorA family magnesium ion transporter|... 25 8.4 >SPBC36.04 |cys11|cys1a|cysteine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 351 Score = 69.7 bits (163), Expect = 2e-13 Identities = 37/73 (50%), Positives = 47/73 (64%) Frame = -1 Query: 241 DAEQKGILKPGKSVIVEPTSGNTGIGLALAAAVRGYRCIIVLPEKMSDEKVNTLVALGAE 62 DAE+KG L G IVE T+GNTGIGLA A RGY+C+I +P S K++TL LGAE Sbjct: 64 DAEKKGKLSRG-GTIVEGTAGNTGIGLAHIARARGYKCVIYMPNTQSQAKIDTLKFLGAE 122 Query: 61 IIRTPTEAAWDSP 23 + P A + +P Sbjct: 123 VHPVPV-APFSNP 134 Score = 50.4 bits (115), Expect = 1e-07 Identities = 24/46 (52%), Positives = 30/46 (65%) Frame = -2 Query: 393 SDILXVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGGSVKDRIA 256 S + IG TPL++L+ L + G C + AK EF NPGGSVKDR A Sbjct: 15 SGFIGAIGRTPLIRLNTLSNETG--CNILAKAEFQNPGGSVKDRAA 58 >SPAC3A12.17c |cys12|cys1b|cysteine synthase Cys12|Schizosaccharomyces pombe|chr 1|||Manual Length = 395 Score = 48.0 bits (109), Expect = 8e-07 Identities = 28/83 (33%), Positives = 44/83 (53%), Gaps = 4/83 (4%) Frame = -2 Query: 492 DAKNGTSNSARKFFTLKDMPHIVRALDRNQKVHSDILX----VIGNTPLVKLSKLPKDEG 325 D +G + A + L+ + +R + DI+ +IGNT +V++ L + G Sbjct: 7 DLASGIAMGAVFMYLLRRLYESLRVKSSADSLEEDIVNGVEGLIGNTKMVRIKSLSQATG 66 Query: 324 LKCEMYAKCEFLNPGGSVKDRIA 256 C++ AK EFLNPG S KDR+A Sbjct: 67 --CDILAKAEFLNPGNSPKDRVA 87 Score = 46.0 bits (104), Expect = 3e-06 Identities = 28/73 (38%), Positives = 40/73 (54%), Gaps = 2/73 (2%) Frame = -1 Query: 259 SFRMFLDAEQKGILKPGKS-VIVEPTSGNTGIGLALAAAVRGYRCIIVLPEKMSDEKVNT 83 + +M AE+ G L P +S + E T+G+TGI +A+ GY I +P S EK + Sbjct: 87 ALQMIRTAEENGDLVPYQSNAVYEGTAGSTGISIAMLCCSLGYDSRIYMPSDQSKEKSDI 146 Query: 82 LVALGAEIIR-TP 47 L LGA + R TP Sbjct: 147 LELLGAHVQRVTP 159 >SPBC1677.03c |||threonine ammonia-lyase|Schizosaccharomyces pombe|chr 2|||Manual Length = 600 Score = 31.1 bits (67), Expect = 0.096 Identities = 14/51 (27%), Positives = 28/51 (54%) Frame = -1 Query: 208 KSVIVEPTSGNTGIGLALAAAVRGYRCIIVLPEKMSDEKVNTLVALGAEII 56 K+ ++ ++GN G+A +A G + IV+P+ + K + LGA ++ Sbjct: 161 KNGVIACSAGNHAQGVAYSARTLGVKATIVMPQNTPEIKWRNVKRLGANVL 211 >SPAC922.03 |||1-aminocyclopropane-1-carboxylate deaminase |Schizosaccharomyces pombe|chr 1|||Manual Length = 338 Score = 27.9 bits (59), Expect = 0.90 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = -2 Query: 372 GNTPLVKLSKLPKDEGLKCEMYAKCEFLNPG 280 G TP+ + +L K G K E++AK E N G Sbjct: 14 GPTPITSMKRLSKTLGGKVEIFAKREDCNSG 44 >SPCC320.14 ||SPCC330.15c|threo-3-hydroxyaspartate ammonia-lyase |Schizosaccharomyces pombe|chr 3|||Manual Length = 323 Score = 27.5 bits (58), Expect = 1.2 Identities = 14/51 (27%), Positives = 26/51 (50%) Frame = -1 Query: 208 KSVIVEPTSGNTGIGLALAAAVRGYRCIIVLPEKMSDEKVNTLVALGAEII 56 K+ ++ +SGN +AL+A + G I++P + KV G ++I Sbjct: 74 KAGVLTFSSGNHAQAIALSAKILGIPAKIIMPLDAPEAKVAATKGYGGQVI 124 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 26.6 bits (56), Expect = 2.1 Identities = 17/60 (28%), Positives = 27/60 (45%) Frame = +3 Query: 3 HAIMLLSGESQAASVGVRIISAPNATSVFTFSSDIFSGNTIMHLYPLTAAASASPIPVLP 182 H + + +S S+ + S NA ++ D+ L + AAAS SP+P LP Sbjct: 402 HVFIEFNSDSGITSLHLHASSNTNADNIIRALGDVAGSARAAGLREI-AAASGSPMPKLP 460 >SPAC23A1.03 |apt1||adenine phosphoribosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 188 Score = 25.8 bits (54), Expect = 3.6 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = -1 Query: 238 AEQKGILKPGKSVIVEPTSGNTGIGLALAA 149 A QKG +KPG+ V++ TG G ALAA Sbjct: 110 AIQKGTIKPGQRVLIVDDILATG-GTALAA 138 >SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.8 bits (54), Expect = 3.6 Identities = 19/64 (29%), Positives = 30/64 (46%) Frame = +3 Query: 36 AASVGVRIISAPNATSVFTFSSDIFSGNTIMHLYPLTAAASASPIPVLPDVGSTMTDLPG 215 AA+ ++ P +T FS + + + AA S P+P +P VG+T T +P Sbjct: 195 AAAKKNKVAVTPQSTLPPGFSPATPAPTSAANTPATIAATSIPPVPNVPLVGAT-TAVPP 253 Query: 216 FKIP 227 IP Sbjct: 254 LSIP 257 >SPAC12G12.15 |sif3||Sad1 interacting factor 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 25.8 bits (54), Expect = 3.6 Identities = 13/50 (26%), Positives = 26/50 (52%) Frame = -2 Query: 477 TSNSARKFFTLKDMPHIVRALDRNQKVHSDILXVIGNTPLVKLSKLPKDE 328 T+ +++K L + H V + + + + D+L L+KL LP+D+ Sbjct: 88 TTKTSQKLVLLPEN-HAVNSFNEEESNYEDLLTPSDAYNLIKLENLPRDK 136 >SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 24.6 bits (51), Expect = 8.4 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +2 Query: 242 QKHAEAMRSLTDPPGLRNSHFAYISHFNPS 331 Q+ A++ ++ T P R++HF H NPS Sbjct: 49 QQVADSTQNPTSKPKSRHAHFHETVHENPS 78 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 24.6 bits (51), Expect = 8.4 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = -1 Query: 100 DEKVNTLVALGAEIIRTPTEAAWDSPESNIIA 5 + V++ AL ++++R P E + ESNIIA Sbjct: 1015 ESAVDSNEALFSKVLRRPIEKETGADESNIIA 1046 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 24.6 bits (51), Expect = 8.4 Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = +2 Query: 215 LQNTLLFCIQKHAEAMRSLTDPP 283 L N + FC++K EA + PP Sbjct: 928 LDNWIQFCLEKDGEAPKEAPPPP 950 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 24.6 bits (51), Expect = 8.4 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = -1 Query: 406 SEGTFRYFXCNRKYTTGEVIQIT*R*RIEM 317 S+GTF +RK TTGE+ I +++M Sbjct: 571 SKGTFGTVYLSRKNTTGEIYAIKVLRKVDM 600 >SPAC17A2.14 ||SPAC17G6.01|CorA family magnesium ion transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 24.6 bits (51), Expect = 8.4 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +2 Query: 188 GFYYD*LARLQNTLLFCIQKHA 253 GFYY AR+QN F +KH+ Sbjct: 12 GFYYKQNARMQNRPRFSDRKHS 33 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,315,641 Number of Sequences: 5004 Number of extensions: 50289 Number of successful extensions: 138 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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