BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf1073
(375 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 26 1.7
SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|... 25 2.9
SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 25 2.9
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 25 3.9
SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|ch... 25 3.9
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 5.1
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 24 6.8
SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3' ss-tail|S... 24 6.8
SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces... 24 6.8
SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom... 24 9.0
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 26.2 bits (55), Expect = 1.7
Identities = 16/53 (30%), Positives = 22/53 (41%)
Frame = +1
Query: 133 NKRFERSQVLAFVVEWIDENKELEWESTFSTSRQHESFRHVQNSSDISKGTFP 291
NK +ER + A VVE +E E S +++ D KGT P
Sbjct: 989 NKTYERENIDAGVVESFVVGTNIEGEQYASELKENSLLTGELKFGDCEKGTVP 1041
>SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 537
Score = 25.4 bits (53), Expect = 2.9
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = -2
Query: 356 NEFVIFKEDSVPMTEIMKMLDEGKVPFDMSEEFCT 252
N+FVI+ ED++ ++++ L K + E CT
Sbjct: 239 NQFVIYLEDTLASLDVVESLSPKKNVRFWTSELCT 273
>SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 25.4 bits (53), Expect = 2.9
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +2
Query: 80 NASTTGRSRGKPRGLLSRTKDSKGAKSLPLLSNG 181
+A TTG S GKP G + T KG + G
Sbjct: 264 SAPTTGFSFGKPAGQAASTATDKGTTTTSSAGTG 297
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 25.0 bits (52), Expect = 3.9
Identities = 16/53 (30%), Positives = 22/53 (41%)
Frame = +2
Query: 77 NNASTTGRSRGKPRGLLSRTKDSKGAKSLPLLSNG*TKTKSWNGNPPSVPLGS 235
NN+ G PR SR + +SNG + +S NP +V GS
Sbjct: 327 NNSGNNRNESGHPRFRNSRRNYNNQGAYPTYMSNGRSANQSPRNNPQNVNNGS 379
>SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 401
Score = 25.0 bits (52), Expect = 3.9
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +1
Query: 136 KRFERSQVLAFVVEWIDENKELEWESTFSTSRQHESFRHVQNSSDI 273
KRFE Q+++ V++W D +E +S + S H +S+ +
Sbjct: 341 KRFEILQLISSVLDWNDTQREQTGLQRPGSSVNNWSIPHSASSNSL 386
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 24.6 bits (51), Expect = 5.1
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +1
Query: 157 VLAFVVEWIDENKELEWESTFSTSRQHESFRHVQNSSDISKGTFP 291
VL V ++D L ++ + QHE + + ++ + KG+FP
Sbjct: 70 VLERDVRYLDSKIALLIQNRMAQEEQHEFAKRLNDNYNAVKGSFP 114
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 24.2 bits (50), Expect = 6.8
Identities = 20/77 (25%), Positives = 37/77 (48%), Gaps = 6/77 (7%)
Frame = +1
Query: 40 NENILEIHVRNLE*CINNGAIQREA----KRLIVKNKRFERSQVLAF--VVEWIDENKEL 201
N N L ++NLE +N+ I+ E+ + L+ + RS +L+ ID+ +
Sbjct: 581 NSNTLSEQIKNLESELNSSKIKNESLLNERNLLKEMLATSRSSILSHNSSAGNIDDKMKS 640
Query: 202 EWESTFSTSRQHESFRH 252
EST + +E +R+
Sbjct: 641 IDESTRELEKNYEVYRN 657
>SPCC1322.01 ||SPCC23B6.06|3'-5' exonuclease for RNA 3'
ss-tail|Schizosaccharomyces pombe|chr 3|||Manual
Length = 957
Score = 24.2 bits (50), Expect = 6.8
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -3
Query: 370 LSAIRMNLSYLKKTPCQ*LKL*RCSTKEKYLLICRKSS 257
L + + +YL K C LKL C K+ C SS
Sbjct: 9 LQNVNLESNYLLKRVCLSLKLSPCKLTRKFHHACPSSS 46
>SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 279
Score = 24.2 bits (50), Expect = 6.8
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +2
Query: 188 KTKSWNGNPPSVPLGSMSLLGMYRT 262
KT SW P VPL S +G+ R+
Sbjct: 187 KTSSWKVVVPLVPLSIASWIGLSRS 211
>SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 569
Score = 23.8 bits (49), Expect = 9.0
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = +2
Query: 164 PLLSNG*TKTKSWNGNPPSVPLGSMSLLGMYRTLPTYQ 277
P S T ++ PPS+P S G TLP Y+
Sbjct: 196 PRRSTAATLESAYEERPPSLPRRKPSRPGTAITLPPYE 233
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,583,257
Number of Sequences: 5004
Number of extensions: 32227
Number of successful extensions: 83
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 81
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 83
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 120195862
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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