BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0985 (627 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 127 1e-30 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 80 3e-16 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 72 8e-14 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 71 2e-13 SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi... 29 0.42 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.2 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 3.9 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 3.9 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 5.1 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 25 6.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 127 bits (307), Expect = 1e-30 Identities = 54/72 (75%), Positives = 62/72 (86%) Frame = +2 Query: 41 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASXGKYV 220 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+ GKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 221 PRAILVDLEPGT 256 PRA+LVDLEPGT Sbjct: 61 PRAVLVDLEPGT 72 Score = 109 bits (263), Expect = 2e-25 Identities = 47/62 (75%), Positives = 55/62 (88%) Frame = +1 Query: 256 MDSVRSGPFGQIFRPDNFVF*QSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQG 435 MD+V+SG FG +FRPDN ++ QSGAGN WAKGHYTEGAEL D+VLDVVR+EAE+CD LQG Sbjct: 73 MDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQG 132 Query: 436 FQ 441 FQ Sbjct: 133 FQ 134 Score = 71.7 bits (168), Expect = 8e-14 Identities = 31/41 (75%), Positives = 37/41 (90%) Frame = +3 Query: 504 QREYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENT 626 + EYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+ Sbjct: 156 REEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENS 196 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 79.8 bits (188), Expect = 3e-16 Identities = 33/69 (47%), Positives = 50/69 (72%) Frame = +2 Query: 44 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASXGKYVP 223 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 224 RAILVDLEP 250 RAIL+DLEP Sbjct: 63 RAILIDLEP 71 Score = 52.4 bits (120), Expect = 5e-08 Identities = 22/68 (32%), Positives = 43/68 (63%), Gaps = 2/68 (2%) Frame = +1 Query: 241 LGARHMDSVRSGPFGQIFRPDNFVF*QSG--AGNNWAKGHYTEGAELVDSVLDVVRKEAE 414 L R ++++ S +G ++ P+N + ++G AGNNWA G Y+ + + ++D++ +EA+ Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127 Query: 415 SCDCLQGF 438 D L+GF Sbjct: 128 GSDSLEGF 135 Score = 42.3 bits (95), Expect = 6e-05 Identities = 20/38 (52%), Positives = 27/38 (71%), Gaps = 1/38 (2%) Frame = +3 Query: 513 YPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVEN 623 YP +I+ TYSV P S VSD VV+PYN+ L++ +L N Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLN 198 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 71.7 bits (168), Expect = 8e-14 Identities = 29/61 (47%), Positives = 44/61 (72%) Frame = +1 Query: 256 MDSVRSGPFGQIFRPDNFVF*QSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQG 435 +D VR+GP+ +F P+ V + A NN+A+GHYT G E++DSVL+ +R+ A++C LQG Sbjct: 79 IDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQG 138 Query: 436 F 438 F Sbjct: 139 F 139 Score = 61.7 bits (143), Expect = 8e-11 Identities = 30/76 (39%), Positives = 42/76 (55%), Gaps = 6/76 (7%) Frame = +2 Query: 41 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 202 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 203 SXGKYVPRAILVDLEP 250 GK+VPR+I VDLEP Sbjct: 61 GQGKFVPRSIYVDLEP 76 Score = 41.1 bits (92), Expect = 1e-04 Identities = 17/39 (43%), Positives = 27/39 (69%) Frame = +3 Query: 510 EYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENT 626 EY + +SV P+P+VS +VVEPYN+ L+ H ++N+ Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNS 202 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 70.5 bits (165), Expect = 2e-13 Identities = 29/61 (47%), Positives = 42/61 (68%) Frame = +1 Query: 256 MDSVRSGPFGQIFRPDNFVF*QSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQG 435 +D VR+GP+ +F P+ + + A NN+A+GHYT G ELVD V D +R+ A++C LQG Sbjct: 75 IDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQG 134 Query: 436 F 438 F Sbjct: 135 F 135 Score = 67.7 bits (158), Expect = 1e-12 Identities = 33/72 (45%), Positives = 43/72 (59%), Gaps = 2/72 (2%) Frame = +2 Query: 41 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASXGK 214 MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 215 YVPRAILVDLEP 250 YVPR+I VDLEP Sbjct: 61 YVPRSIYVDLEP 72 Score = 39.1 bits (87), Expect = 5e-04 Identities = 16/37 (43%), Positives = 25/37 (67%) Frame = +3 Query: 510 EYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVE 620 EY + +SV P+P+VS +VVEPYN+ L+ H ++ Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLD 196 >SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schizosaccharomyces pombe|chr 3|||Manual Length = 649 Score = 29.5 bits (63), Expect = 0.42 Identities = 15/54 (27%), Positives = 27/54 (50%) Frame = -1 Query: 381 IDELSTLRVVSLGPVVAGAGLSEDEVVRTEDLSERSRADRVHVPGSKSTRMARG 220 ++++S V+ +G G G++ D R ++ + D SKST+MA G Sbjct: 62 VEDISKFDVLIIGGGATGTGVAVDASTRGLNVCLLEKTDFASETSSKSTKMAHG 115 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 2.2 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 80 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 181 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.2 bits (55), Expect = 3.9 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 343 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 432 A+GH G ELV + D +RK++E+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 26.2 bits (55), Expect = 3.9 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -2 Query: 416 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCQKTKLSGRKICPK 279 ++A+ RTTS T+ +PS L P P + S R+ CPK Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 5.1 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +1 Query: 295 RPDNFVF*QSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 426 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 25.4 bits (53), Expect = 6.8 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 274 GPFGQIFRPDNFVF*QSGAG-NNWAKGHYTEGAEL 375 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,362,002 Number of Sequences: 5004 Number of extensions: 44011 Number of successful extensions: 133 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 115 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 129 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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