Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf0913
(391 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 124 2e-29
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 66 6e-12
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 66 6e-12
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 66 6e-12
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.068
At2g41620.1 68415.m05143 nucleoporin interacting component famil... 30 0.63
At2g26060.1 68415.m03129 transducin family protein / WD-40 repea... 30 0.63
At3g58770.1 68416.m06550 expressed protein ; expression support... 28 1.9
At1g24050.1 68414.m03036 expressed protein 28 1.9
At5g23260.2 68418.m02722 MADS-box protein, putative 28 2.6
At4g39756.1 68417.m05630 kelch repeat-containing F-box family pr... 27 3.4
At4g25170.1 68417.m03624 expressed protein 27 3.4
At4g21880.1 68417.m03164 pentatricopeptide (PPR) repeat-containi... 27 3.4
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 27 3.4
At3g55130.1 68416.m06122 ABC transporter family protein breast c... 27 4.5
At3g46120.1 68416.m04991 calcineurin-like phosphoesterase family... 27 5.9
At1g64210.1 68414.m07274 leucine-rich repeat transmembrane prote... 27 5.9
At5g19220.1 68418.m02289 glucose-1-phosphate adenylyltransferase... 26 7.8
At4g19940.1 68417.m02921 F-box family protein contains Pfam PF00... 26 7.8
At2g48050.1 68415.m06014 expressed protein ; expression supporte... 26 7.8
At1g63170.1 68414.m07139 zinc finger (C3HC4-type RING finger) fa... 26 7.8
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 124 bits (300), Expect = 2e-29
Identities = 54/78 (69%), Positives = 64/78 (82%)
Frame = -1
Query: 253 SPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSG 74
SP AQ+YR+E LYEGP DD+ A I++CDP PLM+YVSKM+P SDKGRF+AFGRVF+G
Sbjct: 342 SPHTAQRYRVENLYEGPLDDQYANAIRNCDPNGPLMLYVSKMIPASDKGRFFAFGRVFAG 401
Query: 73 KVVTGQKARIMGPNFTPG 20
KV TG K RIMGPN+ PG
Sbjct: 402 KVSTGMKVRIMGPNYIPG 419
Score = 50.8 bits (116), Expect = 3e-07
Identities = 21/44 (47%), Positives = 34/44 (77%)
Frame = -2
Query: 387 LLKKIGVTIKHEDSDKDGKALLKVVMRSWLPAGEALLQMIAIHL 256
+L K+GV++K+++ + GK L+K VM++WLPA ALL+M+ HL
Sbjct: 297 MLAKLGVSMKNDEKELMGKPLMKRVMQTWLPASTALLEMMIFHL 340
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 66.5 bits (155), Expect = 6e-12
Identities = 30/77 (38%), Positives = 44/77 (57%)
Frame = -1
Query: 253 SPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSG 74
SP A +++ Y G D + CDP PLM+ V+K+ P SD F FGRV+SG
Sbjct: 440 SPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSG 499
Query: 73 KVVTGQKARIMGPNFTP 23
++ TGQ R++G ++P
Sbjct: 500 RLQTGQSVRVLGEGYSP 516
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 66.5 bits (155), Expect = 6e-12
Identities = 30/77 (38%), Positives = 44/77 (57%)
Frame = -1
Query: 253 SPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSG 74
SP A +++ Y G D + CDP PLM+ V+K+ P SD F FGRV+SG
Sbjct: 454 SPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSG 513
Query: 73 KVVTGQKARIMGPNFTP 23
++ TGQ R++G ++P
Sbjct: 514 RLQTGQSVRVLGEGYSP 530
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 66.5 bits (155), Expect = 6e-12
Identities = 30/77 (38%), Positives = 44/77 (57%)
Frame = -1
Query: 253 SPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRVFSG 74
SP A +++ Y G D + CDP PLM+ V+K+ P SD F FGRV+SG
Sbjct: 454 SPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSG 513
Query: 73 KVVTGQKARIMGPNFTP 23
++ TGQ R++G ++P
Sbjct: 514 RLQTGQSVRVLGEGYSP 530
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 33.1 bits (72), Expect = 0.068
Identities = 17/45 (37%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = -2
Query: 387 LLKKIGVTIK-HEDSDKDGKALLKVVMRSWLPAGEALLQMIAIHL 256
++K ++I E +KD K +L+ VM WLP +A+L M HL
Sbjct: 303 VIKSFNLSIPPRELQNKDPKNVLQSVMSRWLPLSDAVLSMAVKHL 347
Score = 27.1 bits (57), Expect = 4.5
Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 2/34 (5%)
Frame = -1
Query: 103 FYAFGRVFSGKVVTGQKARIMGPNFTP--G*SSH 8
F AF R+FSG + GQ+ ++ + P G SSH
Sbjct: 440 FLAFARIFSGVLRAGQRVFVITALYDPLKGESSH 473
>At2g41620.1 68415.m05143 nucleoporin interacting component family
protein contains Pfam profile PF04097: Nucleoporin
interacting component
Length = 861
Score = 29.9 bits (64), Expect = 0.63
Identities = 17/68 (25%), Positives = 32/68 (47%)
Frame = +2
Query: 29 KVWSHDASFLSGNNLARENASKGVETTFVGGRHHLAHVHHQWGFRITALDTNGSFIIVGS 208
K+W + ++ R+ SK + +G RHHL H H + F + + T+ + +G
Sbjct: 251 KIWQLVQAITGEDSAVRQGVSKRMALA-IGARHHLQHGHEK--FIMDTIQTHPTQAALGG 307
Query: 209 LI*HLHTI 232
+ +L I
Sbjct: 308 SVGNLQRI 315
>At2g26060.1 68415.m03129 transducin family protein / WD-40 repeat
family protein contains 7 WD-40 repeats (PF00400);
similar to WD40-repeat containing protein Ciao 1
(SP:O76071) [Homo sapiens]
Length = 352
Score = 29.9 bits (64), Expect = 0.63
Identities = 18/68 (26%), Positives = 30/68 (44%)
Frame = +3
Query: 150 SGASGSQLLIPMAASSSWGPSYNISIRYFWATTGDVNEWQSSEAELHQQATKSASQPSAK 329
+G + +L + S GPSYN+ ++ A DVN Q S E ++ ++ K
Sbjct: 285 AGDNAIRLFVDSKHDSVDGPSYNLLLKKNKAHENDVNSVQWSPGEGNRLLASASDDGMVK 344
Query: 330 LCHLCRNP 353
+ L P
Sbjct: 345 IWQLATKP 352
>At3g58770.1 68416.m06550 expressed protein ; expression supported
by MPSS
Length = 771
Score = 28.3 bits (60), Expect = 1.9
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -1
Query: 268 CHSFTSPVVAQKYRMEMLYEGPHD 197
C SF+ PV A +R +LY+ P D
Sbjct: 319 CPSFSQPVSADMHRGTLLYQSPFD 342
>At1g24050.1 68414.m03036 expressed protein
Length = 173
Score = 28.3 bits (60), Expect = 1.9
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -1
Query: 112 KGRFYAFGRVFSGKVVTGQKARIMGPNFTPG 20
+G +A G ++S KV+TG + R +G PG
Sbjct: 15 EGEKFAVGNIYSVKVITGDEFRGIGTKPRPG 45
>At5g23260.2 68418.m02722 MADS-box protein, putative
Length = 252
Score = 27.9 bits (59), Expect = 2.6
Identities = 11/39 (28%), Positives = 24/39 (61%)
Frame = +1
Query: 85 RVQRRRNDLCRRSAPSCSRTSSVGLQDHSS*YQWQLHHR 201
+V+ R+N+L ++ + SR + +D+++ Y+W HR
Sbjct: 136 KVRERKNELMQQQLENLSRKRRMLEEDNNNMYRWLHEHR 174
>At4g39756.1 68417.m05630 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 374
Score = 27.5 bits (58), Expect = 3.4
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -1
Query: 268 CHSFTSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAP 152
C SF S +++ + +E LY G H+D + ++ D P
Sbjct: 47 CKSFCSLILSMELYVERLYLGTHEDVLHVCLQLPDRRLP 85
>At4g25170.1 68417.m03624 expressed protein
Length = 344
Score = 27.5 bits (58), Expect = 3.4
Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
Frame = +3
Query: 102 KRPLSEVGTILLTYIISGASGSQLLIPMAASSSWGPSYNISIRYFWATTG-DVNEWQSSE 278
K+ S G+ LTY S ASG L +P+AA+S W S F + G D EW S E
Sbjct: 59 KQRSSSSGSNNLTY--SAASGLLLNLPVAATSGWSGGGPFS---FVNSGGYDDFEWVSEE 113
Query: 279 AE 284
+
Sbjct: 114 ED 115
>At4g21880.1 68417.m03164 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 845
Score = 27.5 bits (58), Expect = 3.4
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 129 WCRPPTKVVSTPLDAFSLARLLPDK 55
WC+P TK+ T L + ++PDK
Sbjct: 801 WCKPYTKLTLTLLFVVEVVAIVPDK 825
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 27.5 bits (58), Expect = 3.4
Identities = 15/64 (23%), Positives = 28/64 (43%)
Frame = -1
Query: 271 DCHSFTSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAF 92
D H F+ K+++E +Y DD I D A + ++V + V +K +
Sbjct: 620 DPHLFSFAFNRGKFQVEEIYNFAQDDLLTEDIYFLDTHAEVFVWVGQCVEPKEKQTVFEI 679
Query: 91 GRVF 80
G+ +
Sbjct: 680 GQKY 683
>At3g55130.1 68416.m06122 ABC transporter family protein breast
cancer resistance protein 1 BCRP1, Mus musculus,
EMBL:NP_036050
Length = 725
Score = 27.1 bits (57), Expect = 4.5
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +2
Query: 287 SPAGNQERITTFSKALPSLSESSCLIVTPIFLR 385
+P G QER+T F+ +P++ CL P+F++
Sbjct: 463 TPRGAQERLTLFAFVVPTMF-YCCLDNVPVFIQ 494
>At3g46120.1 68416.m04991 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 388
Score = 26.6 bits (56), Expect = 5.9
Identities = 15/47 (31%), Positives = 27/47 (57%)
Frame = +3
Query: 108 PLSEVGTILLTYIISGASGSQLLIPMAASSSWGPSYNISIRYFWATT 248
PL+E G+ ++TY I+ + GS +A +SS+ +N + Y + T
Sbjct: 76 PLNEDGSNVVTYWIANSDGSDNKSALATTSSY-RYFNYTSGYLYHAT 121
>At1g64210.1 68414.m07274 leucine-rich repeat transmembrane protein
kinase, putative contains 1 predicted transmembrane
domain; similar to receptor-like protein kinase
(GI:4008006) [Arabidopsis thaliana]; similar to
receptor-like kinase RHG1 (GI:21239382) [Glycine max];
similar to receptor-like protein kinase 3 (GI:13506810)
[Lycopersicon esculentum]
Length = 587
Score = 26.6 bits (56), Expect = 5.9
Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +2
Query: 29 KVWSHDASFLSGNNLARE-NASKGVETTFVGGRHHL 133
K+ D+S GN +R+ N +G + F GGR+HL
Sbjct: 264 KLRKRDSSSPPGNWTSRDDNTEEGGKIIFFGGRNHL 299
>At5g19220.1 68418.m02289 glucose-1-phosphate adenylyltransferase
large subunit 1 (APL1) / ADP-glucose pyrophosphorylase
(ADG2) identical to SP|P55229
Length = 522
Score = 26.2 bits (55), Expect = 7.8
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +1
Query: 121 SAPSCSRTSSVGLQDH 168
SAPSC R+SS GL H
Sbjct: 12 SAPSCIRSSSTGLTRH 27
>At4g19940.1 68417.m02921 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain;similar to F-box protein Fbx8
(GI:6164735) [Homo
sapiens]|8737673|gb|AV566223.1|AV566223
Length = 411
Score = 26.2 bits (55), Expect = 7.8
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 76 QRKRVQRRRNDLCRRSAP 129
+R+R +RRR DLC+ P
Sbjct: 14 ERRRTKRRRRDLCKSRQP 31
>At2g48050.1 68415.m06014 expressed protein ; expression supported
by MPSS
Length = 1500
Score = 26.2 bits (55), Expect = 7.8
Identities = 11/38 (28%), Positives = 16/38 (42%)
Frame = +3
Query: 153 GASGSQLLIPMAASSSWGPSYNISIRYFWATTGDVNEW 266
G G+ L P S G S S +FW + + + W
Sbjct: 356 GNQGAGFLHPKTGGSESGSSRKFSFGHFWGSIKESHRW 393
>At1g63170.1 68414.m07139 zinc finger (C3HC4-type RING finger)
family protein low similarity to SP|Q06003 Goliath
protein (G1 protein) {Drosophila melanogaster}; contains
Pfam profile PF00097: Zinc finger, C3HC4 type (RING
finger)
Length = 381
Score = 26.2 bits (55), Expect = 7.8
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +1
Query: 79 RKRVQRRRNDLCRRSAPSCSRTSS 150
R+R RRR DL RS+ S S +SS
Sbjct: 128 RRRNSRRRRDLSPRSSSSSSSSSS 151
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,580,746
Number of Sequences: 28952
Number of extensions: 202136
Number of successful extensions: 604
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 604
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 557595584
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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