Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fbVf0885
         (721 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi...    85   3e-17
At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--t...    38   0.009
At1g10980.1 68414.m01260 expressed protein  ; expression support...    30   1.8  
At5g19850.1 68418.m02358 hydrolase, alpha/beta fold family prote...    28   5.4  

>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
           histidine--tRNA ligase, putative similar to SP|P12081
           Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
           ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
           PF00587: tRNA synthetase class II core domain (G, H, P,
           S and T), PF03129: Anticodon binding domain
          Length = 479

 Score = 85.4 bits (202), Expect = 3e-17
 Identities = 36/65 (55%), Positives = 46/65 (70%)
 Frame = +2

Query: 488 KGNKRLQPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKD 667
           KG +    +QM +R      I  VF+RHGA  +DTPVFEL++ L GKYGEDSKL+YD+ D
Sbjct: 46  KGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMGKYGEDSKLVYDIAD 105

Query: 668 QGGEI 682
           QGGE+
Sbjct: 106 QGGEL 110


>At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--tRNA
           ligase identical to histidyl-tRNA synthetase
           [Arabidopsis thaliana] GI:3659909
          Length = 486

 Score = 37.5 bits (83), Expect = 0.009
 Identities = 20/63 (31%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
 Frame = +2

Query: 488 KGNKRLQPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKDVLTGKYGEDSK-LIYDLK 664
           KG +   P+ M +RN + +    V R +G E +D PV E + +   K GE+ +  +Y  +
Sbjct: 73  KGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCFE 132

Query: 665 DQG 673
           D+G
Sbjct: 133 DRG 135



 Score = 27.9 bits (59), Expect = 7.2
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = +3

Query: 426 ALKAQLVTEDAGPQKFTLKTPKGTRDY 506
           ++ A LVTE+   +K  +  PKGTRD+
Sbjct: 52  SIVAPLVTEEDFHKKIDVNPPKGTRDF 78


>At1g10980.1 68414.m01260 expressed protein  ; expression supported
           by MPSS
          Length = 516

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 13/37 (35%), Positives = 22/37 (59%)
 Frame = -3

Query: 596 QVYQYTQLRVS*KL**FYPIHYYVLSFAVVVVSCSLW 486
           +VY Y+ L    K+    P  +Y+LS+A++V+ C  W
Sbjct: 418 EVYLYSSLSQFWKVKWIVPTLWYILSYAMLVLICLFW 454


>At5g19850.1 68418.m02358 hydrolase, alpha/beta fold family protein
           low similarity to hydrolase [Terrabacter sp. DBF63]
           GI:14196240; contains Pfam profile PF00561: hydrolase,
           alpha/beta fold family
          Length = 359

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 10/40 (25%), Positives = 23/40 (57%)
 Frame = +3

Query: 297 LLTFFKTHVMARRLCNAVQRNCLTKNVTTSSCRKVKEEVS 416
           +++F +TH + + +  A+ R+   + + + SC  V +E S
Sbjct: 28  IISFSETHFLRQSISTAIVRSPTKRGIVSVSCSSVTDEAS 67


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,104,058
Number of Sequences: 28952
Number of extensions: 308850
Number of successful extensions: 637
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 622
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 637
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)