BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0792 (775 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 42 1e-04 SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 31 0.18 SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosacchar... 27 3.0 SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 26 5.2 SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||... 26 5.2 SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 26 6.9 SPAC17G8.09 |shg1||Csp15 family protein|Schizosaccharomyces pomb... 26 6.9 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 41.5 bits (93), Expect = 1e-04 Identities = 16/44 (36%), Positives = 26/44 (59%) Frame = -3 Query: 134 DPEHHPDPEKFDPDRFLDENKHKIKPFTFLPFGAGPRNCIGSRF 3 D + +P+PE F+PDR+ + P ++ FG GP C+G R+ Sbjct: 421 DSKVYPEPETFNPDRWAPNGLAEQSPKNWMVFGNGPHVCLGQRY 464 >SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 31.1 bits (67), Expect = 0.18 Identities = 20/78 (25%), Positives = 34/78 (43%) Frame = -1 Query: 502 RKWTQDELTAQVFIFFLAGFESSANGLTLCIHELALNPEAQEKLYEAIIKFKEEKGPLTY 323 R + E+ + +AG +SA + + L PE E L+E + E L + Sbjct: 271 RPLKEHEIAGMMIALLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGENLELKF 330 Query: 322 DNIGELKYLDCVLNETSR 269 D ++ L+ V+ ET R Sbjct: 331 DQYKDMPLLNYVIQETLR 348 Score = 28.3 bits (60), Expect = 1.3 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = -3 Query: 50 FLPFGAGPRNCIGSRF 3 +LPFGAG CIG +F Sbjct: 432 YLPFGAGRHRCIGEQF 447 >SPAC6C3.06c |||P-type ATPase, calcium transporting|Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 27.1 bits (57), Expect = 3.0 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = -1 Query: 481 LTAQVFIFFLAGFESSANGLTLCIHELALN 392 L Q+F F+L GFE L +C L N Sbjct: 921 LIIQLFTFYLIGFEEEGKMLAVCFSCLIFN 950 >SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 512 Score = 26.2 bits (55), Expect = 5.2 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = -2 Query: 708 FPQKVTRFFREIVTSTMDYRLKNNVERPDM--IQLLMDAYKGTLK 580 FP+KV +F+ V +DY N + D+ LL+D + G LK Sbjct: 296 FPEKVAKFYAAEVILALDYLHHNQIVYRDLKPENLLLDRF-GHLK 339 >SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 26.2 bits (55), Expect = 5.2 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = -1 Query: 145 PSKWIPSIILTLRSLTRTDSWMKISTKSNRLLSCHLVLD 29 P++WIP+ T+R + R D + ++ S R + L LD Sbjct: 401 PTRWIPTANRTIRCIRRGD-FQTAASSSKRNCTYFLTLD 438 >SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pombe|chr 2|||Manual Length = 1692 Score = 25.8 bits (54), Expect = 6.9 Identities = 10/23 (43%), Positives = 17/23 (73%) Frame = +3 Query: 486 SWVHFLTFPLVLTSLLS*QNLHF 554 S+ +F+TFPL++T L + L+F Sbjct: 533 SYNNFVTFPLIITELSQLETLNF 555 >SPAC17G8.09 |shg1||Csp15 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 25.8 bits (54), Expect = 6.9 Identities = 10/33 (30%), Positives = 18/33 (54%) Frame = -3 Query: 170 DIVYNSVNSIQMDPEHHPDPEKFDPDRFLDENK 72 +++YNS+ I ++ H D EK + +D K Sbjct: 96 EVIYNSLEQIGIEQIEHEDEEKLLNSKTMDGRK 128 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,283,859 Number of Sequences: 5004 Number of extensions: 70034 Number of successful extensions: 206 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 198 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 206 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 373338084 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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