Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf0388
(670 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g28910.2 68417.m04132 expressed protein 35 0.056
At4g28910.1 68417.m04131 expressed protein 35 0.056
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 33 0.13
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 31 0.69
At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (... 29 2.1
At4g10130.1 68417.m01657 DNAJ heat shock N-terminal domain-conta... 29 2.8
At4g02650.1 68417.m00360 epsin N-terminal homology (ENTH) domain... 29 2.8
At5g41220.1 68418.m05009 glutathione S-transferase, putative sim... 29 3.7
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 29 3.7
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 28 6.5
At1g74590.1 68414.m08640 glutathione S-transferase, putative sim... 27 8.5
>At4g28910.2 68417.m04132 expressed protein
Length = 425
Score = 34.7 bits (76), Expect = 0.056
Identities = 22/75 (29%), Positives = 39/75 (52%)
Frame = +3
Query: 186 SDASISDRRQAVRSEHRHLQVPRRKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYEA 365
++A S + V E R +RK+G G ND++ E D + +H+ + KA+ V
Sbjct: 93 TEAEASTKPLWVEDESRKEAGNKRKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTAT 152
Query: 366 DEELKAKKHEDFSKN 410
DE A ++ED +++
Sbjct: 153 DEGSTA-ENEDVAES 166
>At4g28910.1 68417.m04131 expressed protein
Length = 425
Score = 34.7 bits (76), Expect = 0.056
Identities = 22/75 (29%), Positives = 39/75 (52%)
Frame = +3
Query: 186 SDASISDRRQAVRSEHRHLQVPRRKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYYEA 365
++A S + V E R +RK+G G ND++ E D + +H+ + KA+ V
Sbjct: 93 TEAEASTKPLWVEDESRKEAGNKRKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTAT 152
Query: 366 DEELKAKKHEDFSKN 410
DE A ++ED +++
Sbjct: 153 DEGSTA-ENEDVAES 166
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 33.5 bits (73), Expect = 0.13
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +1
Query: 157 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGASTGSPGP 279
P++ PFG++PVLV + +S AI RY+ S GP
Sbjct: 42 PEYLAIQPFGKIPVLVDGDYKIFESRAIMRYIAEKYRSQGP 82
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 31.1 bits (67), Expect = 0.69
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +1
Query: 157 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGASTGSPG 276
P F PFGQ+PV + G + +S AI Y+ S G
Sbjct: 79 PSFLAINPFGQVPVFLDGGLKLTESRAISEYIATVHKSRG 118
>At1g54960.1 68414.m06277 NPK1-related protein kinase, putative
(ANP2) similar to protein kinase [Nicotiana tabacum]
gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
protein kinase 2, partial cds GI:2342424
Length = 596
Score = 29.5 bits (63), Expect = 2.1
Identities = 13/30 (43%), Positives = 22/30 (73%)
Frame = +2
Query: 551 LEVQYPAFKKVLQSVLTQPKVKAFLDLGGH 640
+EV +P++ + ++ LT+ K+KAFLD GH
Sbjct: 470 MEVDHPSYSED-ENELTESKIKAFLDDKGH 498
>At4g10130.1 68417.m01657 DNAJ heat shock N-terminal
domain-containing protein low similarity to SP|Q9QYI7
DnaJ homolog subfamily B member 8 Mus musculus,
SP|P50025 Chaperone protein dnaJ Legionella pneumophila;
contains Pfam profile PF00226 DnaJ domain
Length = 174
Score = 29.1 bits (62), Expect = 2.8
Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
Frame = +3
Query: 177 SVRSDASISDRRQAVRSEHRHLQVPRRKYGLAGANDEEAF-EIDQNVEFLHDIRAKAAAV 353
SV+ DAS + R + RS H + ++D+E F +I + E L D A+ V
Sbjct: 17 SVKEDASYEEIRNSYRSAILHSHPDKLNNTSRSSSDDEKFLKIQKAWEVLSD--AELRVV 74
Query: 354 YYEADEELKAKKHE 395
Y D +L++ +H+
Sbjct: 75 Y---DNDLRSSRHD 85
>At4g02650.1 68417.m00360 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to Chain A,
Calm-N N-Terminal Domain Of Clathrin Assembly Lymphoid
Myeloid Leukaemia Protein, Pi(4,5)p2 Complex
(GP:13399999) {Homo sapiens}; supporting cDNA
gi|26451912|dbj|AK118440.1|
Length = 611
Score = 29.1 bits (62), Expect = 2.8
Identities = 22/70 (31%), Positives = 34/70 (48%)
Frame = +3
Query: 180 VRSDASISDRRQAVRSEHRHLQVPRRKYGLAGANDEEAFEIDQNVEFLHDIRAKAAAVYY 359
VR+ A D R R + R ++K G G D ++ E D + +DIR+KA V
Sbjct: 141 VRTYALYLDERLDYRMQGRR---GKKKSGGGGGGDGDSGEEDDHRGTSNDIRSKAIVVKS 197
Query: 360 EADEELKAKK 389
+ E+K +K
Sbjct: 198 KPVAEMKTEK 207
>At5g41220.1 68418.m05009 glutathione S-transferase, putative
similar to emb|CAA10662
Length = 590
Score = 28.7 bits (61), Expect = 3.7
Identities = 14/36 (38%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +1
Query: 157 PDFKPKTPFGQMPVLVIDGK-QYAQSTAICRYLGAS 261
P+FK P G++P ++DGK + ++S AI YL ++
Sbjct: 43 PEFKDINPMGKVPA-IVDGKLKLSESHAILIYLSSA 77
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 28.7 bits (61), Expect = 3.7
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +1
Query: 157 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGASTG 267
P+F P G++PVL +S AI RY+ G
Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAIARYVSRKNG 79
>At2g30860.1 68415.m03761 glutathione S-transferase, putative
identical to GB:Y12295
Length = 215
Score = 27.9 bits (59), Expect = 6.5
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +1
Query: 157 PDFKPKTPFGQMPVLVIDGKQYAQSTAICRYLGASTGSPGP 279
P + PFG +P +V + +S A+ RY+ S GP
Sbjct: 42 PAYLALQPFGTVPAVVDGDYKIFESRAVMRYVAEKYRSQGP 82
>At1g74590.1 68414.m08640 glutathione S-transferase, putative
similar to putative glutathione S-transferase
GB:CAA10060 [Arabidopsis thaliana]; contains Pfam
profile: PF00043 Glutathione S-transferases
Length = 232
Score = 27.5 bits (58), Expect = 8.5
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = +1
Query: 187 QMPVLVIDGKQYAQSTAICRYLGAS-TGSP 273
++PVLV DGK A+S I Y+ + T SP
Sbjct: 56 KIPVLVHDGKPVAESLVILEYIDETWTNSP 85
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,217,918
Number of Sequences: 28952
Number of extensions: 251926
Number of successful extensions: 696
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 678
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 696
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -