BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0350 (622 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g44030.1 68414.m05079 DC1 domain-containing protein contains ... 28 4.3 At5g24770.1 68418.m02924 vegetative storage protein 2 (VSP2) ide... 28 5.7 At2g34920.1 68415.m04287 ubiquitin-protein ligase-related contai... 28 5.7 At5g64813.1 68418.m08152 GTP-binding protein-related contains we... 27 7.6 At4g35470.1 68417.m05041 leucine-rich repeat family protein simi... 27 7.6 >At1g44030.1 68414.m05079 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 597 Score = 28.3 bits (60), Expect = 4.3 Identities = 9/31 (29%), Positives = 18/31 (58%) Frame = -1 Query: 460 PIHYAMKIKCLISPTQVALKRHRYHLSMITS 368 P + + +KC+ P + + RH++ +S TS Sbjct: 446 PCDFVVHLKCISLPRVIRISRHQHRISFTTS 476 >At5g24770.1 68418.m02924 vegetative storage protein 2 (VSP2) identical to SP|O82122 Vegetative storage protein 2 precursor {Arabidopsis thaliana}; contains Pfam profile PF03767: HAD superfamily (subfamily IIIB) phosphatase Length = 265 Score = 27.9 bits (59), Expect = 5.7 Identities = 10/36 (27%), Positives = 16/36 (44%) Frame = +3 Query: 207 CNPYYMDFLFVDRYNHYPSLVICIRLFVYKKRIAFR 314 C Y D+L + Y S +C + Y K +A + Sbjct: 74 CKDYVEDYLITSKQYQYDSKTVCKEAYFYAKGLALK 109 >At2g34920.1 68415.m04287 ubiquitin-protein ligase-related contains weak similarity to Ubiquitin-protein ligase E3 Mdm2 (EC 6.3.2.-) (p53-binding protein Mdm2) (Oncoprotein Mdm2) (Double minute 2 protein) (Swiss-Prot:P23804) [Mus musculus] Length = 785 Score = 27.9 bits (59), Expect = 5.7 Identities = 17/59 (28%), Positives = 26/59 (44%) Frame = -3 Query: 497 PRHRAKDTGQKVTHPLRNENQMSHIPDAGGSKTSPVSLVNDYKHIICLTNESGLDSLIY 321 P H T Q LR+ + D S V L N +K C+ NE+ +++L+Y Sbjct: 688 PTHNLHSTLQLEMSELRDS--VKTCLDVNASLQKSVHLENPFKRKCCVCNETQVETLLY 744 >At5g64813.1 68418.m08152 GTP-binding protein-related contains weak similarity to Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Swiss-Prot:P51159) [Homo sapiens] Length = 342 Score = 27.5 bits (58), Expect = 7.6 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -3 Query: 494 RHRAKDTGQKVTHPLRNENQMSHIPDAGGSKTSPVSLVNDYKHII 360 R R ++ +++ PL + ++ + D+G KTS V L+N I+ Sbjct: 5 RERERENKEQILAPLCGQVRVLVVGDSGVGKTSLVHLINKGSSIV 49 >At4g35470.1 68417.m05041 leucine-rich repeat family protein similar to Leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8) (SP:Q9UQ13 ){Homo sapiens},PIR:T12704; contains Pfam PF00560: Leucine Rich Repeat domains Length = 549 Score = 27.5 bits (58), Expect = 7.6 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 1/54 (1%) Frame = -3 Query: 485 AKDTGQKVTHPLRNENQMSHIPDAGGSKTSPVSLVNDYKHIICLTNE-SGLDSL 327 AK Q++ + Q+ +PD+ G +S SL HI+ L N GL SL Sbjct: 218 AKKATQEINLQNKLTEQLEWLPDSLGKLSSLTSLDLSENHIVVLPNTIGGLSSL 271 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,846,448 Number of Sequences: 28952 Number of extensions: 252690 Number of successful extensions: 529 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 510 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 529 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1255974912 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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