Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf0314
(434 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g25757.1 68418.m03055 expressed protein 58 2e-09
At5g25754.1 68418.m03054 expressed protein 58 2e-09
At2g21840.1 68415.m02595 CHP-rich zinc finger protein, putative 30 0.77
At1g67890.1 68414.m07752 protein kinase family protein contains ... 28 3.1
At1g54260.1 68414.m06186 histone H1/H5 family protein contains P... 28 3.1
At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 27 5.5
At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 27 5.5
At3g06620.1 68416.m00769 protein kinase family protein contains ... 27 5.5
At3g29320.1 68416.m03682 glucan phosphorylase, putative similar ... 27 7.2
At5g27790.1 68418.m03332 protein kinase family protein contains ... 26 9.5
At5g02070.1 68418.m00128 protein kinase-related contains eukaryo... 26 9.5
>At5g25757.1 68418.m03055 expressed protein
Length = 514
Score = 58.4 bits (135), Expect = 2e-09
Identities = 25/60 (41%), Positives = 42/60 (70%), Gaps = 2/60 (3%)
Frame = +2
Query: 260 GEFESCFTFACPKFLSACPPPI--EPGANYGRDAVKHQTQVFMDEVRQQKMLPTIRSYLK 433
G ++ F++ACPKF++ PP EP NY +DA + Q ++F+ EV+QQ++L +R++LK
Sbjct: 364 GIYDELFSYACPKFITPSAPPTFEEPLVNYNQDAYRLQLKMFLYEVKQQQLLSGVRTFLK 423
Score = 56.0 bits (129), Expect = 1e-08
Identities = 34/71 (47%), Positives = 46/71 (64%), Gaps = 2/71 (2%)
Frame = +3
Query: 39 RTLSSALLYMQRTKQLFSSRSYQNDQINKQTEQMYHLLAICLVLHPQ--CVDESIQQVLR 212
R + LLY+ +TKQ + +S Q +Q+ K+ EQMY LLA+ L L PQ VDES+ LR
Sbjct: 287 REFNKILLYIFKTKQ-YHQKSPQYEQLLKKNEQMYALLALSLSLCPQTKLVDESVNSQLR 345
Query: 213 EKNYHEKMFKM 245
+K Y EKM +M
Sbjct: 346 DK-YGEKMMRM 355
>At5g25754.1 68418.m03054 expressed protein
Length = 514
Score = 58.4 bits (135), Expect = 2e-09
Identities = 25/60 (41%), Positives = 42/60 (70%), Gaps = 2/60 (3%)
Frame = +2
Query: 260 GEFESCFTFACPKFLSACPPPI--EPGANYGRDAVKHQTQVFMDEVRQQKMLPTIRSYLK 433
G ++ F++ACPKF++ PP EP NY +DA + Q ++F+ EV+QQ++L +R++LK
Sbjct: 364 GIYDELFSYACPKFITPSAPPTFEEPLVNYNQDAYRLQLKMFLYEVKQQQLLSGVRTFLK 423
Score = 56.0 bits (129), Expect = 1e-08
Identities = 34/71 (47%), Positives = 46/71 (64%), Gaps = 2/71 (2%)
Frame = +3
Query: 39 RTLSSALLYMQRTKQLFSSRSYQNDQINKQTEQMYHLLAICLVLHPQ--CVDESIQQVLR 212
R + LLY+ +TKQ + +S Q +Q+ K+ EQMY LLA+ L L PQ VDES+ LR
Sbjct: 287 REFNKILLYIFKTKQ-YHQKSPQYEQLLKKNEQMYALLALSLSLCPQTKLVDESVNSQLR 345
Query: 213 EKNYHEKMFKM 245
+K Y EKM +M
Sbjct: 346 DK-YGEKMMRM 355
>At2g21840.1 68415.m02595 CHP-rich zinc finger protein, putative
Length = 746
Score = 29.9 bits (64), Expect = 0.77
Identities = 19/68 (27%), Positives = 37/68 (54%), Gaps = 4/68 (5%)
Frame = +3
Query: 36 YRTLSSALLYMQRTKQLFSSRSYQNDQINKQT---EQMYHLLAIC-LVLHPQCVDESIQQ 203
Y++ L Y++ T+++ R Q I K+T ++ Y+L C L H +C++ I +
Sbjct: 239 YKSHEHRLFYVESTRRVADERVKQPCLICKETVVYDRFYYLCVECDLKCHTKCLE--IPE 296
Query: 204 VLREKNYH 227
+ +K+YH
Sbjct: 297 YVIKKSYH 304
>At1g67890.1 68414.m07752 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 765
Score = 27.9 bits (59), Expect = 3.1
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +1
Query: 25 RRRHTAPSLLRCYTCNALNNCSPPVPTRMIRSTN 126
RR H A + R N L++CSPP+ R ++S+N
Sbjct: 584 RRIHMASDIAR--GMNYLHHCSPPIIHRDLKSSN 615
>At1g54260.1 68414.m06186 histone H1/H5 family protein contains Pfam
domain, PF00538: linker histone H1 and H5 family
Length = 197
Score = 27.9 bits (59), Expect = 3.1
Identities = 10/36 (27%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +3
Query: 78 KQLFSSRSYQNDQ-INKQTEQMYHLLAICLVLHPQC 182
K+L ++ + + ++ ++K E+ + +L +CL +H QC
Sbjct: 153 KRLIAAEAVEEEERMHKLVEESHTMLQLCLEIHQQC 188
>At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 27.1 bits (57), Expect = 5.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 276 ALLSRAPNSYRRVPRPLNPVPTMAVMLSNTRPKSLWTRSVN 398
+L R P S R+ RP + MAV +P ++WT N
Sbjct: 423 SLCGRGPRSSLRILRPGLAITEMAVSQLPGQPSAVWTVKKN 463
>At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A
subunit region (PF03178); contains weak WD-40 repeat
(PF00400); similar to Splicing factor 3B subunit 3
(SF3b130)/spliceosomal protein/Splicing factor 3B
subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens,
EMBL:HSAJ1443_1
Length = 1214
Score = 27.1 bits (57), Expect = 5.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 276 ALLSRAPNSYRRVPRPLNPVPTMAVMLSNTRPKSLWTRSVN 398
+L R P S R+ RP + MAV +P ++WT N
Sbjct: 423 SLCGRGPRSSLRILRPGLAITEMAVSQLPGQPSAVWTVKKN 463
>At3g06620.1 68416.m00769 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 773
Score = 27.1 bits (57), Expect = 5.5
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +1
Query: 25 RRRHTAPSLLRCYTCNALNNCSPPVPTRMIRSTN 126
RR H A + R N L++CSPP+ R ++S+N
Sbjct: 591 RRIHMALDIAR--GMNYLHHCSPPIIHRDLKSSN 622
>At3g29320.1 68416.m03682 glucan phosphorylase, putative similar to
alpha-glucan phosphorylase, L isozyme 1 precursor
GB:P04045 from [Solanum tuberosum] (J. Biochem. 106 (4),
691-695 (1989))
Length = 962
Score = 26.6 bits (56), Expect = 7.2
Identities = 22/72 (30%), Positives = 37/72 (51%), Gaps = 5/72 (6%)
Frame = +3
Query: 27 PTP-YRTLSSALLYMQRTK---QLFSSRSYQNDQINKQTEQMYHLLAICLVLHPQCVDES 194
P P Y+T ++ L + TK + F SY + + + E +++ IC VL+P DES
Sbjct: 299 PIPGYKTKTTINLRLWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEKICFVLYPG--DES 356
Query: 195 IQ-QVLREKNYH 227
+ + LR K +
Sbjct: 357 TEGKALRLKQQY 368
>At5g27790.1 68418.m03332 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 327
Score = 26.2 bits (55), Expect = 9.5
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +3
Query: 177 QCVDESIQQVLREKNYHEKMFKMQYGT 257
QC ++Q +K Y E M M+Y T
Sbjct: 79 QCYGNGVKQRFNDKGYVEYMIPMEYAT 105
>At5g02070.1 68418.m00128 protein kinase-related contains eukaryotic
protein kinase domain, INTERPRO:IPR000719
Length = 657
Score = 26.2 bits (55), Expect = 9.5
Identities = 22/63 (34%), Positives = 29/63 (46%), Gaps = 5/63 (7%)
Frame = -1
Query: 197 DGFINALRMQDQTNGQ*MVHLLGLFVD----LIILVGTGGEQLFSALH-V*QRRREGAVW 33
D +N +R+ Q N + +V LLG VD L+I LF LH R + W
Sbjct: 402 DQILNEVRILCQVNHRSLVRLLGCCVDLELPLLIYEFIPNGTLFEHLHGSSDRTWKPLTW 461
Query: 32 RRR 24
RRR
Sbjct: 462 RRR 464
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,078,416
Number of Sequences: 28952
Number of extensions: 243586
Number of successful extensions: 675
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 671
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 683042040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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