BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0233 (553 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g53640.1 68418.m06663 F-box family protein contains F-box dom... 31 0.39 At4g03200.1 68417.m00437 expressed protein contains Pfam PF03190... 29 1.6 At1g35680.1 68414.m04436 50S ribosomal protein L21, chloroplast ... 29 2.1 At5g63590.1 68418.m07983 flavonol synthase, putative similar to ... 28 3.6 At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 28 3.6 At2g37420.1 68415.m04589 kinesin motor protein-related 28 3.6 At1g71320.1 68414.m08232 S locus F-box-related / SLF-related con... 28 3.6 At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 tr... 28 4.8 At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 tr... 28 4.8 At2g34780.1 68415.m04270 expressed protein 27 8.3 >At5g53640.1 68418.m06663 F-box family protein contains F-box domain Pfam:PF00646 Length = 917 Score = 31.5 bits (68), Expect = 0.39 Identities = 17/44 (38%), Positives = 26/44 (59%), Gaps = 2/44 (4%) Frame = -1 Query: 475 NTNVRSSWVFKPLWESNKLYFKIWNADSNSYLEFGEK--DANGI 350 +T RS W++ P E N LYF D N+++ FG+K D+N + Sbjct: 520 STRWRSLWLWLPCLELNSLYF----PDFNAFVSFGDKFFDSNRV 559 >At4g03200.1 68417.m00437 expressed protein contains Pfam PF03190: Protein of unknown function, DUF255 Length = 818 Score = 29.5 bits (63), Expect = 1.6 Identities = 14/35 (40%), Positives = 19/35 (54%) Frame = -1 Query: 463 RSSWVFKPLWESNKLYFKIWNADSNSYLEFGEKDA 359 R+S + K ES K YF + N+ Y+E EK A Sbjct: 533 RASKILKAEPESTKYYFPVVNSQPEDYIEVAEKAA 567 >At1g35680.1 68414.m04436 50S ribosomal protein L21, chloroplast / CL21 (RPL21) identical to 50S ribosomal protein L21, chloroplast precursor (CL21) [Arabidopsis thaliana] SWISS-PROT:P51412 Length = 220 Score = 29.1 bits (62), Expect = 2.1 Identities = 10/34 (29%), Positives = 21/34 (61%) Frame = +2 Query: 374 EFKVGIGVRIPNFEVELVALPQWLEYPTAPHVSI 475 +++ IG R PN + + + + EYP +P+V++ Sbjct: 182 KYRRNIGHRQPNTRIRITGITGYEEYPASPNVAV 215 >At5g63590.1 68418.m07983 flavonol synthase, putative similar to SP|Q96330 Flavonol synthase 1 (EC 1.14.11.-) (FLS 1) {Arabidopsis thaliana}; contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family Length = 308 Score = 28.3 bits (60), Expect = 3.6 Identities = 13/44 (29%), Positives = 24/44 (54%), Gaps = 3/44 (6%) Frame = -1 Query: 463 RSSWV---FKPLWESNKLYFKIWNADSNSYLEFGEKDANGIQPL 341 R++WV F +W +++ K W + Y+E E+ A+ I+ L Sbjct: 97 RNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNEEYASHIKKL 140 >At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (ATHIM) identical to SP|P34881 DNA (cytosine-5)-methyltransferase AthI (EC 2.1.1.37) {Arabidopsis thaliana} Length = 1534 Score = 28.3 bits (60), Expect = 3.6 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +1 Query: 280 HFCRAPNTTGIFRCWLCPSLLVVEYHWHPFRRI 378 H C+A N + RC L P+ ++H P R++ Sbjct: 1381 HICKAMNELNLIRCKLIPTRPGADWHDLPKRKV 1413 >At2g37420.1 68415.m04589 kinesin motor protein-related Length = 1039 Score = 28.3 bits (60), Expect = 3.6 Identities = 15/23 (65%), Positives = 20/23 (86%) Frame = -1 Query: 232 ILSRLTSKRTAMMSNSFSEN*RE 164 ILS+LTSK+TAM+S++ S N RE Sbjct: 774 ILSKLTSKKTAMISDA-SSNIRE 795 >At1g71320.1 68414.m08232 S locus F-box-related / SLF-related contains F-box domain Pfam:PF00646; contains TIGRFAM TIGR01640: F-box protein interaction domain; similar to S locus F-box (SLF)-S2-like protein (GI:13161528) [Antirrhinum hispanicum] Length = 392 Score = 28.3 bits (60), Expect = 3.6 Identities = 8/23 (34%), Positives = 17/23 (73%) Frame = +2 Query: 56 KIHLLRVDYQQKHSIHAKFWLIG 124 KI+ +++D + + +H KFW++G Sbjct: 332 KIYSIKIDVIRTNQLHEKFWMLG 354 >At2g24420.2 68415.m02918 DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss-Prot:O33600) [Sulfolobus acidocaldarius] Length = 440 Score = 27.9 bits (59), Expect = 4.8 Identities = 12/40 (30%), Positives = 17/40 (42%) Frame = +1 Query: 289 RAPNTTGIFRCWLCPSLLVVEYHWHPFRRIQGRNWSPHSK 408 +A + WL P V HW F+ + G +W H K Sbjct: 197 KAKELMEVHGAWLPPWFAV---HWSSFQTVAGTHWDAHGK 233 >At2g24420.1 68415.m02917 DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss-Prot:O33600) [Sulfolobus acidocaldarius] Length = 440 Score = 27.9 bits (59), Expect = 4.8 Identities = 12/40 (30%), Positives = 17/40 (42%) Frame = +1 Query: 289 RAPNTTGIFRCWLCPSLLVVEYHWHPFRRIQGRNWSPHSK 408 +A + WL P V HW F+ + G +W H K Sbjct: 197 KAKELMEVHGAWLPPWFAV---HWSSFQTVAGTHWDAHGK 233 >At2g34780.1 68415.m04270 expressed protein Length = 1297 Score = 27.1 bits (57), Expect = 8.3 Identities = 21/95 (22%), Positives = 45/95 (47%), Gaps = 2/95 (2%) Frame = -1 Query: 403 NADSNSYLEFGEKDANGIQPLIGSDRASSERFQWYLVPDKNAATSEIFF*FTIASLTILS 224 + D L GEK ++G+ SE+ + + PD+ +S I +++ Sbjct: 614 SGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDI-----TVSKKR 668 Query: 223 RLTSKRTAMMSNS--FSEN*REPMSILNTTVGISS 125 R++ K+ ++ NS F+++ + P +I T +S+ Sbjct: 669 RVSCKKKIIVQNSLEFNQSGKTPGNIAGKTTCLST 703 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,192,433 Number of Sequences: 28952 Number of extensions: 225195 Number of successful extensions: 614 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 606 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 614 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1043173136 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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