BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0199 (436 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.7 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 6.7 SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 24 8.8 SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 24 8.8 SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 24 8.8 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 24.6 bits (51), Expect = 6.7 Identities = 15/36 (41%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Frame = -2 Query: 270 RAAF*TPRF*GASFRRHPSAARGL-PPSTGKRPRSR 166 R+AF R+ G+ R+ + R L PP+T PRSR Sbjct: 78 RSAF-RSRYRGSLLNRNSPSLRSLSPPATPATPRSR 112 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 24.6 bits (51), Expect = 6.7 Identities = 8/25 (32%), Positives = 12/25 (48%) Frame = -3 Query: 287 EFTTRLGLHSKHPDFKERPFGGTLP 213 EF+ LH+ HP + P+ P Sbjct: 175 EFSRAYDLHNAHPSIHDAPYSSPFP 199 >SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 400 Score = 24.2 bits (50), Expect = 8.8 Identities = 8/19 (42%), Positives = 14/19 (73%) Frame = -3 Query: 356 ISXQEPVMPMITNWSSVPI 300 +S +PV P+ N+SS+P+ Sbjct: 319 VSQTQPVNPITPNYSSIPL 337 >SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 24.2 bits (50), Expect = 8.8 Identities = 8/21 (38%), Positives = 12/21 (57%) Frame = -2 Query: 135 KRNLPNTTSPVID*NNGIQCW 73 +RN P+ +P+ D N Q W Sbjct: 638 RRNAPDINNPIADFQNSFQNW 658 >SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces pombe|chr 3|||Manual Length = 429 Score = 24.2 bits (50), Expect = 8.8 Identities = 8/24 (33%), Positives = 16/24 (66%) Frame = -3 Query: 377 EKCEANNISXQEPVMPMITNWSSV 306 +K E NN+ + P++ + TN+ S+ Sbjct: 64 QKFEMNNVQKESPIVSVETNFDSL 87 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,888,949 Number of Sequences: 5004 Number of extensions: 38800 Number of successful extensions: 77 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 76 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 77 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 156095170 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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