Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fbVf0180
(429 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 1.8
At5g42370.1 68418.m05159 expressed protein 29 1.8
At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 1.8
At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 2.3
At5g60000.1 68418.m07524 hypothetical protein 27 4.1
At5g22390.1 68418.m02612 expressed protein 27 4.1
At3g56510.1 68416.m06284 TBP-binding protein, putative similar t... 27 5.4
At2g05210.1 68415.m00549 expressed protein 27 5.4
At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase fa... 27 5.4
At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase fa... 27 5.4
At5g56500.1 68418.m07051 chaperonin, putative similar to SWISS-P... 27 7.1
At3g44430.1 68416.m04774 expressed protein 27 7.1
At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 27 7.1
At3g04340.1 68416.m00459 FtsH protease family protein similar to... 26 9.4
At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containi... 26 9.4
At1g60230.1 68414.m06783 radical SAM domain-containing protein ... 26 9.4
>At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to
autophagy 7 [Arabidopsis thaliana] GI:19912147; contains
Pfam profile PF00899: ThiF family
Length = 697
Score = 28.7 bits (61), Expect = 1.8
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = -1
Query: 246 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 112
P ++G CG +V NH++LL +S+ L+ ++S +R +
Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86
>At5g42370.1 68418.m05159 expressed protein
Length = 447
Score = 28.7 bits (61), Expect = 1.8
Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -2
Query: 185 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 75
P Y + + Q+ +K +P PL + R L W TPS R
Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322
>At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical
to low affinity calcium antiporter CAX2 (GI:1488267)
[Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA)
Family member PMID:11500563
Length = 441
Score = 28.7 bits (61), Expect = 1.8
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Frame = +3
Query: 48 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 179
S++ SL TS + PK P+++ S K +FC L++ +P+ P+
Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86
>At1g72520.1 68414.m08386 lipoxygenase, putative similar to
lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140
[Lycopersicon esculentum], GB:CAB56692 [Arabidopsis
thaliana]
Length = 926
Score = 28.3 bits (60), Expect = 2.3
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -3
Query: 241 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 143
+S + P R ++PYLPS G RTLR+K
Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247
>At5g60000.1 68418.m07524 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 4.1
Identities = 19/70 (27%), Positives = 29/70 (41%), Gaps = 1/70 (1%)
Frame = -2
Query: 335 RGSSPWRPAADMGTNRRDISTYIPHLNFKVRREYPD-TAANAVLFAFRTISPFYRIPWNS 159
+GS PW+P +GT R+ + + + D T + F FYR WN+
Sbjct: 78 QGSRPWQPRTCIGTFYREAHFGMHETPYVLYNSDKDATESKNSCFVVVKDDGFYR--WNN 135
Query: 158 NAQAEKKTLP 129
N + K P
Sbjct: 136 NKRIWDKVFP 145
>At5g22390.1 68418.m02612 expressed protein
Length = 202
Score = 27.5 bits (58), Expect = 4.1
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +3
Query: 120 ERSGKSFLFCLSVRVPWNPIEG 185
+ S KSFL LS PWNP +G
Sbjct: 17 DNSPKSFLDTLSSSSPWNPSKG 38
>At3g56510.1 68416.m06284 TBP-binding protein, putative similar to
TBP-binding protein ABT1 GI:6518527 from [Mus musculus]
Length = 257
Score = 27.1 bits (57), Expect = 5.4
Identities = 17/68 (25%), Positives = 28/68 (41%)
Frame = -2
Query: 212 VLFAFRTISPFYRIPWNSNAQAEKKTLPGPLGGVFRPLWVTPSNTRF*RRGNDY*NGSAA 33
+L + + Y P +S AQ +K G G F WV + +R D NG
Sbjct: 69 ILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQI 128
Query: 32 GFRNRNRI 9
G + ++ +
Sbjct: 129 GGKKKSSV 136
>At2g05210.1 68415.m00549 expressed protein
Length = 364
Score = 27.1 bits (57), Expect = 5.4
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = -3
Query: 241 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHFGLPR 86
+S T C+ +E Y +GF T TLR+KR L S +F PR
Sbjct: 282 DSTATLEAFLCNKDAE-YFWGLGFQDTETLRKKRNWL--LGIRESSNFVAPR 330
>At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 530
Score = 27.1 bits (57), Expect = 5.4
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -1
Query: 168 MELERSGRKENSSRTSRRRLQATLGYPVEHSFLKTRERLL 49
MEL+RS ++ T +RRLQ L +P E S L + +L
Sbjct: 60 MELDRS----DNVHTVKRRLQIALNFPTEESSLTYGDMVL 95
>At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase
family protein low similarity to phosphatidylinositol
4-kinase type-II beta [Homo sapiens] GI:20159767;
contains Pfam profile PF00454: Phosphatidylinositol 3-
and 4-kinase
Length = 650
Score = 27.1 bits (57), Expect = 5.4
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -1
Query: 168 MELERSGRKENSSRTSRRRLQATLGYPVEHSFLKTRERLL 49
MEL+RS ++ T +RRLQ L +P E S L + +L
Sbjct: 60 MELDRS----DNVHTVKRRLQIALNFPTEESSLTYGDMVL 95
>At5g56500.1 68418.m07051 chaperonin, putative similar to
SWISS-PROT:P08927- RuBisCO subunit binding-protein beta
subunit, chloroplast precursor (60 kDa chaperonin beta
subunit, CPN-60 beta) [Pisum sativum]; contains
Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
Length = 597
Score = 26.6 bits (56), Expect = 7.1
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = -3
Query: 247 SAESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHF 98
S S PP R SS + S F T+++ Q++ FP + AA HF
Sbjct: 10 SMGSSLAPPSNRLSSFVS--ISSSSFGRTQSIAQRKARFPKIYAAKQLHF 57
>At3g44430.1 68416.m04774 expressed protein
Length = 206
Score = 26.6 bits (56), Expect = 7.1
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -1
Query: 297 YEPARHLHVHPSPEFQGPQRVSGHRRKCG 211
++P+R + PSP F G Q+ S R+CG
Sbjct: 42 FQPSRI--ISPSPVFNGGQKTSYGGRRCG 68
>At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 676
Score = 26.6 bits (56), Expect = 7.1
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = -3
Query: 202 RSEPYLPSIGFHGTRTLRQKRK 137
R+ PY+P + F G RTL+ +R+
Sbjct: 288 RTFPYVPDMKFSGNRTLKLERQ 309
>At3g04340.1 68416.m00459 FtsH protease family protein similar to
chloroplast FtsH protease [Arabidopsis thaliana]
GI:1483215; contains Pfam profiles PF01434: Peptidase
family M41, PF00004: ATPase AAA family
Length = 960
Score = 26.2 bits (55), Expect = 9.4
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = +3
Query: 39 GTVSIIVPSSLKTSVRRGNPKWPEDA 116
G ++VP +++ +R G KW E+A
Sbjct: 81 GEKEVVVPKAIQLHLRHGWKKWQEEA 106
>At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containing
protein
Length = 964
Score = 26.2 bits (55), Expect = 9.4
Identities = 21/69 (30%), Positives = 25/69 (36%), Gaps = 3/69 (4%)
Frame = -2
Query: 413 GPQSQSLFRSYGSNLPTSLTYIILSTRGSS---PWRPAADMGTNRRDISTYIPHLNFKVR 243
G Q Y S P S +Y T GS+ P P A G +S Y P +V
Sbjct: 519 GSQKSQYGLPYKSGSPNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRGEVN 578
Query: 242 REYPDTAAN 216
YP N
Sbjct: 579 MRYPAATRN 587
>At1g60230.1 68414.m06783 radical SAM domain-containing protein
contains Pfam profile PF04055: radical SAM domain
protein
Length = 458
Score = 26.2 bits (55), Expect = 9.4
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = -3
Query: 133 FPDLSAASSGHFGLPRRTLVFKDEGTIIETVPLP 32
F D+ +AS G R+ L D+G +IETV +P
Sbjct: 161 FKDIRSASDG----TRKILFTLDDGLVIETVVIP 190
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,474,969
Number of Sequences: 28952
Number of extensions: 229178
Number of successful extensions: 678
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 678
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 675111616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -