BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0162 (554 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|ch... 32 0.065 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 5.7 SPAC4F10.12 |fta1|sma1|Sim4 and Mal2 associated |Schizosaccharom... 25 9.9 SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 25 9.9 SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr 2... 25 9.9 SPBC13G1.08c |ash2||Ash2-trithorax family protein|Schizosaccharo... 25 9.9 SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 25 9.9 SPAC4G9.13c |vps26|pep8|retromer complex subunit Vps26|Schizosac... 25 9.9 >SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 31.9 bits (69), Expect = 0.065 Identities = 12/44 (27%), Positives = 24/44 (54%) Frame = +2 Query: 293 QMPRHLISDAHEWINEIPTVPIYYLAKPQPRERAWENHRGKKTL 424 + +H I+ + W+N IP Y L + R+++ EN+ +T+ Sbjct: 309 EFTKHWINRSLSWVNSIPVFGKYLLLSVEERQKSLENYISSETI 352 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 5.7 Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 2/55 (3%) Frame = -3 Query: 270 YSRRLPRLLEFLHVDIQSTGQKSHCVNT--REGHRNALF*LDSRIPLVRASSELT 112 Y RL R+L L + ST H ++ R HR A D+R+ +VR SELT Sbjct: 497 YYVRLARILSQLSSEF-STSLVDHARHSFKRMIHRKAKHEYDTRLLIVRYISELT 550 >SPAC4F10.12 |fta1|sma1|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 280 Score = 24.6 bits (51), Expect = 9.9 Identities = 8/19 (42%), Positives = 11/19 (57%) Frame = +2 Query: 389 RAWENHRGKKTLLSLTLVW 445 + W+NH +T L L L W Sbjct: 169 KIWQNHVNSQTALPLELTW 187 >SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 24.6 bits (51), Expect = 9.9 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +2 Query: 299 PRHLISDAHEWINEIPTVPIYYLAKPQP 382 P LI+D + I ++PT PIY +A P Sbjct: 303 PASLINDLLD-IKKVPTKPIYDMASEYP 329 >SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 479 Score = 24.6 bits (51), Expect = 9.9 Identities = 13/42 (30%), Positives = 18/42 (42%) Frame = -1 Query: 131 VPVLS*PLNGGRTESCRSRTKRNRHDLTARKIRGRPENAGPD 6 VP+ PL+ T+ T RH T + + N GPD Sbjct: 211 VPIFRIPLSDYETDDMDDGTPLQRHVATLNQYQSPIFNLGPD 252 >SPBC13G1.08c |ash2||Ash2-trithorax family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 652 Score = 24.6 bits (51), Expect = 9.9 Identities = 11/16 (68%), Positives = 12/16 (75%) Frame = +2 Query: 335 NEIPTVPIYYLAKPQP 382 NE+PTVPI Y KP P Sbjct: 274 NEVPTVPIRY--KPPP 287 >SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces pombe|chr 3|||Manual Length = 413 Score = 24.6 bits (51), Expect = 9.9 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = +1 Query: 484 DRFARSSLKTTTFICFIT 537 D+FA S L+T F CFI+ Sbjct: 355 DQFAHSFLRTPMFDCFIS 372 >SPAC4G9.13c |vps26|pep8|retromer complex subunit Vps26|Schizosaccharomyces pombe|chr 1|||Manual Length = 298 Score = 24.6 bits (51), Expect = 9.9 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = +1 Query: 85 HDSVRPPFNGQLRTGTDKGN 144 HD V+ F GQ+ DKGN Sbjct: 61 HDGVKIEFIGQIENTYDKGN 80 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,322,807 Number of Sequences: 5004 Number of extensions: 46783 Number of successful extensions: 119 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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