BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0145 (796 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g16980.1 68418.m01989 NADP-dependent oxidoreductase, putative... 30 2.0 At1g76965.1 68414.m08961 glycine-rich protein 29 2.7 At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 4.7 At5g42370.1 68418.m05159 expressed protein 29 4.7 At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 4.7 At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative... 28 6.2 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 6.2 At1g04140.2 68414.m00404 transducin family protein / WD-40 repea... 28 6.2 At1g04140.1 68414.m00403 transducin family protein / WD-40 repea... 28 6.2 At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containi... 28 8.2 At2g02800.2 68415.m00225 protein kinase (APK2b) identical to pro... 28 8.2 At2g02800.1 68415.m00224 protein kinase (APK2b) identical to pro... 28 8.2 >At5g16980.1 68418.m01989 NADP-dependent oxidoreductase, putative strong similarity to probable NADP-dependent oxidoreductase (zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430], Arabidopsis thaliana Length = 239 Score = 29.9 bits (64), Expect = 2.0 Identities = 12/27 (44%), Positives = 18/27 (66%) Frame = -2 Query: 585 RVRIQSET*DDFRECHIKYIQFLRPHI 505 R+RIQ DF + + K+++FL PHI Sbjct: 172 RIRIQGFVVSDFYDEYSKFLEFLHPHI 198 >At1g76965.1 68414.m08961 glycine-rich protein Length = 158 Score = 29.5 bits (63), Expect = 2.7 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -1 Query: 454 PGTGRIRFPSKPDTPRSSEPILIPKLRIQFAD 359 PG + FP KP+ P P +P+L + F D Sbjct: 93 PGAAIVVFPKKPEEPVKVVPTPMPQLNLFFGD 124 >At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to autophagy 7 [Arabidopsis thaliana] GI:19912147; contains Pfam profile PF00899: ThiF family Length = 697 Score = 28.7 bits (61), Expect = 4.7 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -1 Query: 241 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 107 P ++G CG +V NH++LL +S+ L+ ++S +R + Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86 >At5g42370.1 68418.m05159 expressed protein Length = 447 Score = 28.7 bits (61), Expect = 4.7 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 180 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 70 P Y + + Q+ +K +P PL + R L W TPS R Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322 >At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical to low affinity calcium antiporter CAX2 (GI:1488267) [Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 441 Score = 28.7 bits (61), Expect = 4.7 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%) Frame = +1 Query: 43 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 174 S++ SL TS + PK P+++ S K +FC L++ +P+ P+ Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86 >At5g16970.1 68418.m01988 NADP-dependent oxidoreductase, putative (P1) identical to probable NADP-dependent oxidoreductase P1, zeta-crystallin homolog [SP|Q39172][gi:886428], Arabidopsis thaliana; similar to allyl alcohol dehydrogenase [Nicotiana tabacum] GI:6692816; contains Pfam profile PF00107: oxidoreductase, zinc-binding dehydrogenase family Length = 345 Score = 28.3 bits (60), Expect = 6.2 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = -2 Query: 585 RVRIQSET*DDFRECHIKYIQFLRPHI 505 R+RIQ DF + + K+++F+ PHI Sbjct: 278 RIRIQGFVVSDFYDKYSKFLEFVLPHI 304 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 28.3 bits (60), Expect = 6.2 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -3 Query: 236 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 138 +S + P R ++PYLPS G RTLR+K Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247 >At1g04140.2 68414.m00404 transducin family protein / WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to neural cell adhesion molecule 2, large isoform precursor gb|M76710 from Xenopus laevis, and beta transducin from S. cerevisiae gb|Q05946. ESTs gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101, gb|H36304, and gb|N65606 come from Length = 793 Score = 28.3 bits (60), Expect = 6.2 Identities = 17/50 (34%), Positives = 22/50 (44%) Frame = -3 Query: 377 TDPICRLPLPTLFYRLEALHLGDLLRIWVRTGATSPRTSLTEFSRSAESI 228 T LPL L Y L + D RI +G T PR + T F + S+ Sbjct: 289 TSLAAELPLVPLPYLLLPSYSADDPRILYSSGTTGPRNAQTRFQSNQSSV 338 >At1g04140.1 68414.m00403 transducin family protein / WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to neural cell adhesion molecule 2, large isoform precursor gb|M76710 from Xenopus laevis, and beta transducin from S. cerevisiae gb|Q05946. ESTs gb|N65081 gb|Z30910, gb|Z34190, gb|Z34611, gb|R30101, gb|H36304, and gb|N65606 come from Length = 790 Score = 28.3 bits (60), Expect = 6.2 Identities = 17/50 (34%), Positives = 22/50 (44%) Frame = -3 Query: 377 TDPICRLPLPTLFYRLEALHLGDLLRIWVRTGATSPRTSLTEFSRSAESI 228 T LPL L Y L + D RI +G T PR + T F + S+ Sbjct: 289 TSLAAELPLVPLPYLLLPSYSADDPRILYSSGTTGPRNAQTRFQSNQSSV 338 >At2g29140.1 68415.m03542 pumilio/Puf RNA-binding domain-containing protein Length = 964 Score = 27.9 bits (59), Expect = 8.2 Identities = 22/69 (31%), Positives = 26/69 (37%), Gaps = 3/69 (4%) Frame = -2 Query: 408 GPQSQSLFRSYGSNLPTSLTYIILSTRGSS---PWRPAADMGTNRRDISTYIPHRIFKVR 238 G Q Y S P S +Y T GS+ P P A G +S Y P R +V Sbjct: 519 GSQKSQYGLPYKSGSPNSHSYYGSPTFGSNMSYPGSPLAHHGMPNSLMSPYSPMRRGEVN 578 Query: 237 REYPDTAAN 211 YP N Sbjct: 579 MRYPAATRN 587 >At2g02800.2 68415.m00225 protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 Length = 426 Score = 27.9 bits (59), Expect = 8.2 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = -1 Query: 463 STRPGTGRIRFPSKPDTPRSSEPILIPKLRIQFA-DFPYLH 344 ST+PGTG ++ D+PR S ++ K +++ D P LH Sbjct: 368 STKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSYDRPLLH 408 >At2g02800.1 68415.m00224 protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 Length = 426 Score = 27.9 bits (59), Expect = 8.2 Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Frame = -1 Query: 463 STRPGTGRIRFPSKPDTPRSSEPILIPKLRIQFA-DFPYLH 344 ST+PGTG ++ D+PR S ++ K +++ D P LH Sbjct: 368 STKPGTGVGNRQAQIDSPRGSNGSIVQKSPRRYSYDRPLLH 408 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,534,351 Number of Sequences: 28952 Number of extensions: 409442 Number of successful extensions: 1238 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 1188 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1238 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1794809600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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