BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fbVf0142 (599 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putat... 28 4.1 At1g67970.1 68414.m07764 heat shock factor protein, putative (HS... 28 5.4 At1g06460.1 68414.m00684 31.2 kDa small heat shock family protei... 27 7.2 At3g29600.1 68416.m03720 hypothetical protein 27 9.5 At1g35510.1 68414.m04407 expressed protein contains Pfam PF03138... 27 9.5 >At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putative / CPA-FA synthase, putative similar to cyclopropane synthase [Sterculia foetida] GI:21069167; contains Pfam profiles PF02353: Cyclopropane-fatty-acyl-phospholipid synthase, PF01593: amine oxidase, flavin-containing Length = 867 Score = 28.3 bits (60), Expect = 4.1 Identities = 11/36 (30%), Positives = 20/36 (55%) Frame = +3 Query: 462 IQNARRDVEAHLDRGDDAIGFFLNTCITAPKSGYNS 569 + ARR++ H D ++ GFFL+ +T + + S Sbjct: 569 LTQARRNISRHYDLSNELFGFFLDDTMTYSSAVFKS 604 >At1g67970.1 68414.m07764 heat shock factor protein, putative (HSF5) / heat shock transcription factor, putative (HSTF5) identical to heat shock transcription factor 5 (HSF5) SP:Q9S7U5 from [Arabidopsis thaliana]; contains Pfam profile: PF00447 HSF-type DNA-binding domain Length = 374 Score = 27.9 bits (59), Expect = 5.4 Identities = 12/44 (27%), Positives = 22/44 (50%) Frame = +2 Query: 311 GRWXEVPSAGLCLNASKAEASLAESGKDMLTVEPRESGGSKQCD 442 G W ++ +K + ++ + GKD LT+E E G+ + D Sbjct: 286 GAWEKLLLLSPSRKKTKKQENIVKKGKDDLTLEEEEEDGTMELD 329 >At1g06460.1 68414.m00684 31.2 kDa small heat shock family protein / hsp20 family protein contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 285 Score = 27.5 bits (58), Expect = 7.2 Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%) Frame = +3 Query: 360 RPKPA*PNPARICSLWSPESREALNNVTLLVAFRIQNAR-RDVEAHLD 500 RPK P A + ++WSP S A + + +VA + A D+ +D Sbjct: 169 RPKLDLPKLANLGTVWSPRSNVAESTHSYVVAIELPGASINDIRVEVD 216 >At3g29600.1 68416.m03720 hypothetical protein Length = 164 Score = 27.1 bits (57), Expect = 9.5 Identities = 15/42 (35%), Positives = 25/42 (59%), Gaps = 2/42 (4%) Frame = -3 Query: 312 PLGRVHEPNVRNCGSSRTERIT--IATTSHQ*GKTNLSHDGL 193 PL P+ + GS RT++ + I +SH+ G+T+ S DG+ Sbjct: 9 PLVGSDRPDRSSDGSDRTDQSSNGIGRSSHETGRTDRSSDGM 50 >At1g35510.1 68414.m04407 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'growth regulator protein -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 568 Score = 27.1 bits (57), Expect = 9.5 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = -1 Query: 119 ADIEGSKSNVAMNAWLPQ 66 AD+ GS NV M AW P+ Sbjct: 122 ADVNGSSHNVLMEAWKPR 139 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,817,559 Number of Sequences: 28952 Number of extensions: 255431 Number of successful extensions: 548 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 536 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 548 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1187288784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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